Method for acquiring banana somatic cell hybrid by using protoplast asymmetric fusion technology

Abstract

The invention discloses a method for getting soma hybrid of banana by using a non-symmetric protoplast fusion technology, wherein, protoplast is got by enzymolyzing banana embryonic suspension cells in enzymolysis fluid, the receiving protoplast is treated by iodacetamide, the supplying protoplast is illuminated by an ultraviolet lamp, after the receiving and the supplying protoplast are mixed, they are fused by Polyethylene Glycol method. The product from the fusion is suspended in the liquid protoplast culture media, and cultured, and the cell group got is transferred onto the embryo induction culture media to culture till the embryo sprouts, then complete soma hybrid tree is got. For the invention, only fused product can regenerate tree, which reduces the labor during culturing and forsieving and verification after getting sprouts; meanwhile, the formula for the culture media used in the invention is simple and cheap, solves the problem of complexity in culturing fused product after fusing banana protoplast.

Description

technical field The invention belongs to the technical field of banana somatic cell hybridization, and in particular relates to a method for obtaining banana somatic cell hybrids by using a protoplast asymmetric fusion technology. Background technique At present, cultivated banana diseases are rampant, facing a wide variety of disease threats including fungi, bacteria, viruses and physiological diseases and nematodes, especially the No. 4 physiological race of banana fusarium wilt (Fusarium oxysporum f.sp.Cybense Snyder&Hansed, race4), has become a devastating disease of bananas in my country, coupled with the attack of cold damage and other abiotic adversities, banana production is facing severe challenges, and it is urgent to cultivate new varieties of bananas with good quality and disease resistance and stress resistance. Because cultivated bananas are highly sterile and have no seeds, it is difficult to obtain new varieties through traditional hybrid breeding methods, and...

AI Technical Summary

Problems solved by technology

These technical systems mainly have the deficiencies in the following aspects: ① The protoplast liquid medium used for fusion product culture has a complex composition, with as many as 37 components, and contains many expensive and uncommonly used reagents, such as vitamin A (VA) , vitamin D3 (V D3 ), zeatin, etc.; ②The medium formulations used in each culture stage of fusion products are different, which makes the protoplast fusion work cumbersome and time-consuming; ③After protoplast fusion, how to screen and identify somatic hybrids requires a lot of work

Due to the above-mentioned deficiencies in the prior art, the further application of cultivating banana new varieties by somatic cell hybridization technology is limited

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