Immunochromatographic test strip for quickly detecting trichomonas vaginalis and its preparation method

A technology of Trichomonas vaginalis and immunochromatography, applied in the direction of measuring devices, analysis materials, instruments, etc., to achieve high sensitivity and strong specificity

Inactive Publication Date: 2008-03-19
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are currently no immunochromatographic test strips for the rapid detection of Trichomonas vaginalis

Method used

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  • Immunochromatographic test strip for quickly detecting trichomonas vaginalis and its preparation method
  • Immunochromatographic test strip for quickly detecting trichomonas vaginalis and its preparation method

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Embodiment Construction

[0015] The immunochromatographic test strip for rapid detection of Trichomonas vaginalis includes a liner, a sample cushion layer arranged on the liner plate and overlapped sequentially, a binding cushion layer sprayed with gold-labeled monoclonal antibody, a coated point-membrane monoclonal antibody sprayed and sheep The NC membrane and water-absorbing layer of the anti-mouse secondary antibody, the gold-labeled monoclonal antibody is one strain of the colloidal gold-labeled monoclonal antibody AP33, and the membrane-dotted monoclonal antibody is the other strain of the monoclonal antibody AP33. First select any strain as the spotting monoclonal antibody, and then combine it with the other 4 strains of monoclonal antibody (gold standard), and select the compatible pair with the strongest reaction. In this experiment, 4A2 spotting film was used, and 4F8 was used as the gold standard for the strongest reaction. The strongest gold-labeled antibody 4F8 is the gold label, and then ...

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Abstract

The present invention relates to a testing bar, in particular to an immunity chromatographic test bar and the preparation method thereof for testing the trichomonas vaginalis. The present invention adopts the colloids metal immunity chromatographic test technology which is developed in the recent years and provided with the properties of strong specificity, high sensitivity, convenience and speediness. The immunity chromatographic test bar for quickly testing the trichomonas vaginalis is made by using 5 stems of monoclonal antibodies of the anti-recombination AP33 albumen screened out by the hybridoma technology, thus optimizing the best reaction mode by the monovalent parting of the different stems.

Description

technical field [0001] The invention relates to a diagnostic test strip, in particular to an immunochromatographic test strip for rapidly detecting Trichomonas vaginalis and a preparation method thereof. Background technique [0002] Trichomonas vaginalis (T.v) is a flagellate that parasitizes the urinary and reproductive tracts of men and women, and can cause a variety of reproductive system diseases such as trichomonas vaginitis, prostatitis, and urethritis. Trichomoniasis is a common sexually transmitted disease (STD) with a global distribution and a general susceptibility to the population. The disease is also associated with male infertility. In addition to causing genitourinary tract infection, T.v is also one of the risk factors for HIV infection and cervical cancer. At present, routine clinical examination methods at home and abroad include saline smear method, culture detection method and immunological method (such as ELISA, direct fluorescent antibody test (DFA),...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/569
Inventor 潘长旺
Owner WENZHOU MEDICAL UNIV
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