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Method for preparing self-body large intestine cancer vaccine

A colorectal cancer and autologous technology, applied in the field of tumor vaccines, can solve the problem of unsatisfactory killing effect and achieve the effect of increasing the killing rate

Inactive Publication Date: 2008-04-30
史增祥
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] The present invention provides a method for preparing an autologous colorectal cancer vaccine in order to solve the problem of unsatisfactory killing effect of using the patient's own DC and colorectal tumor antigens to prepare autologous anti-tumor vaccines in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Induction and culture of DC in vitro

[0040] Take 40ml of peripheral venous blood, add 40ml of PBS to dilute, pipette 20ml of diluted blood, place 1cm above the lymphocyte separation medium, slowly add to 20ml of lymphocyte separation medium along the wall of the centrifuge tube, a total of four tubes. Centrifuge at 1500rpm for 30min and carefully absorb the interfacial cells with a capillary pipette, add 1 / 3 of PBS and mix well. Centrifuge at 1500rpm for 15min, discard the supernatant, add 5ml of erythrocyte lysate, mix well and let stand for 10min. Centrifuge at 1000rpm for 5min and discard the supernatant. The precipitate was mixed with 2ml RPMI1640, and the number of cells and the proportion of living cells were recorded. Centrifuge at 800rpm for 5min, mix the precipitate with the lymphocyte culture medium evenly, and adjust the concentration to 2×10 6 / ml. placed in a culture flask, 5% CO 2 , incubate in an incubator at 37°C for 2 hours, shake the culture ...

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PUM

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Abstract

The invention relates to a tumor vaccine, in particular to a process for prreparing an autogenous colon cancer vaccine. The invention resolves the problem that in the prior art, autologous DC and colon tumor antigen of a patient are employed to prepare the autogenous colon cancer vaccine, which is unfavorable in killing effect. Steps include that the DC is externally induced and cultivated, obtaining the mature DC, the autogenous colon cancer vaccine is taken as the tumor antigen for preparing, the prepared tumor antigen and the prepared DC are mixed and cultivated by 24 hours, thereby obtaining the DC modified by the autogenous colon cancer vaccine, and the DC and lymphocyte are mixed and cultivated according to the quantitative proportion of 1 to 10-1000. The kill rate of the final obtained autogenous colon cancer vaccine to colon cancer cells is remarkedly increased.

Description

technical field [0001] The invention relates to a tumor vaccine, in particular to a preparation method of an autologous colorectal cancer vaccine. Background technique [0002] Dendritic cells (dendritic cells, DC) were first reported by American scholars Setinman and Cohn in the 1970s, and were named for their mature cells with many dendritic processes. DC is a professional antigen presenting cell (APC, antigen presenting cell) in vivo and plays an important role in T cell immune response. Cellular immunity mediated by T cells plays a dominant role in the body's anti-tumor immunity, and its sensitization, activation, and expansion require APCs to present antigen-specific antigens and provide co-stimulatory signals for T cell activation. Other APCs, such as macrophages and B cells, only stimulate activated or memory T cells and cannot induce initial immune responses, while DCs are the only ones that can stimulate the proliferation of initial T cells except for the uptake, p...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K35/38A61P35/00A61K35/15A61K35/17
Inventor 冯毅
Owner 史增祥
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