Preparation method and application of hyaluronic acid modified ruthenium nano photo-thermal material

A hyaluronic acid modification, hyaluronic acid technology, applied in nano-optics, nanotechnology, nanotechnology and other directions, can solve the problem of few reports on photothermal effect research, achieve good photothermal conversion efficiency and photothermal stability, Good NIR absorption, good targeting effect

Inactive Publication Date: 2018-06-08
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the transition metal ruthenium, it has achieved remarkable anti-cancer effects. However, there are few reports on the photothermal effect of noble metal ruthenium (Ru) nanomaterials.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] A method for preparing a hyaluronic acid-modified ruthenium nano-photothermal material, comprising the steps of:

[0027] (1) Preparation of hyaluronic acid-cysteine: Dissolve hyaluronic acid (HA) monomer (2.274 g, 1.69 mmol) in 100 mL of double distilled water, and then add EDC (0.7668 g , 1.13 mmol) and NSH (0.4604g, 1.13 mmol), activated the carboxyl group at room temperature for 1~2 h, then added cysteine ​​hydrochloride (Lcys, 0.3968 g, 2.26 mmol), stirred at room temperature for 24 h, Then use a dialysis bag with a molecular weight cut-off of 8000-14000 Da to dialyze for 48 hours, change the water every 8 hours, and then freeze-dry;

[0028] (2) Preparation of ruthenium hyaluronic acid nano-photothermal material: the prepared HA-Lcys (0.1005 g) and ruthenium trichloride (0.1005 g) were dissolved in 50 mL of twice distilled water, and then mixed evenly and stirred at room temperature 12 h; then add sodium borohydride (0.1033 g) to it under ultrasonic conditions, a...

experiment example 1

[0030] Prepare the HA-Lcys-Ru prepared in the example into a 0.10-0.60 mg / mL aqueous solution, take 1 mL of the solution, irradiate it with an 808nm laser (1 W, spot diameter 5 mm), and record the solution with an infrared thermal imager temperature as a function of light duration.

[0031] From the results of photothermal heating, it can be seen that with the increase of light time, the temperature of HA-Lcys-Ru aqueous solution increased significantly, while that of pure water only increased by 1.2 ℃ under the same light conditions. It is proved that the photothermal material of the present invention has a good photothermal heating effect.

experiment example 2

[0033] The HA-Lcys-Ru prepared in the example was formulated into a 600 μg / mL aqueous solution, and 1.0 W / cm 2 When the free HA-Lcys-Ru solution was irradiated with the laser power cycle, after 5 cycles, the HA-Lcys-Ru solution could repeatedly reach the temperature similar to the first time (ΔT=36 ℃). The experimental results showed that the synthesized HA- Lcys-Ru nanoparticles show good light and thermal stability.

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PUM

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Abstract

The invention discloses a preparation method and application of a hyaluronic acid modified ruthenium nano photo-thermal material. The preparation method comprises the following steps: firstly, enabling hyaluronic acid and cysteine hydrochloride to carry out an amidation reaction so as to obtain cysteine modified hyaluronic acid; secondly, enabling the cysteine modified hyaluronic acid to react with ruthenium chloride under reaction of sodium borohydride, thereby obtaining a water-soluble hyaluronic acid modified ruthenium nano photo-thermal material. The preparation method is easy in preparation raw material obtaining, simple in process and small in environment pollution; meanwhile, the hyaluronic acid has the property of targeting cancer cells with over-expressed CD44, so that a high-efficiency photo-thermal material with a targeting function can be prepared, and the material has an application value in cancer treatment; due to targeting photo-thermal treatment of the hyaluronic acidmodified ruthenium nano photo-thermal material, the cancer cell killing rate is increased, and the material has wide application prospects in photo-thermal treatment, functional materials, tissue engineering, and the like.

Description

technical field [0001] The invention relates to a photothermal therapy reagent, which belongs to the technical field of biopolymer materials, and in particular to a preparation method and application of a hyaluronic acid-modified ruthenium nanometer photothermal material. Background technique [0002] Phototherapy, consisting of photothermal therapy (PTT) and photodynamic therapy (PDT), is expected to improve in situ cancer treatment compared with other conventional therapies due to its noninvasive and local therapeutic effect under special laser irradiation. PTT is a new strategy for cancer treatment. The main principle of photothermal therapy is to use photothermal reagents to enter or reach the cellular microenvironment through cellular endocytosis, absorb light of a specific wavelength, and convert the absorbed light energy into heat to cause local environment The temperature rises, which in turn kills the cancerous cells. Studies have shown that near-infrared light h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/61A61P35/00B82Y5/00B82Y20/00A61L27/20A61L27/50C08B37/08
CPCA61K41/0052A61L27/20A61L27/50B82Y5/00B82Y20/00C08B37/0072C08L5/08
Inventor 沈星灿王文龙蒋邦平
Owner GUANGXI NORMAL UNIV
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