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Method for breeding phalaenopsis aphrodite by bioreactor method

A technology of bioreactor and Phalaenopsis, which is applied in the field of large-scale propagation of Phalaenopsis, can solve the problem that it is difficult to form large-scale production of fine varieties, and achieve the effects of shortening the reproduction cycle, consistent genetic background, and small variation in clones

Inactive Publication Date: 2008-06-11
李杰
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Problems solved by technology

[0003] The invention provides a method for rapidly multiplying Phalaenopsis through a bioreactor, which can solve the problem that it is difficult for fine varieties to form large-scale production in a relatively short period of time

Method used

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Embodiment Construction

[0007] The present invention is a kind of method for mass-propagating Phalaenopsis by means of a bioreactor. It adopts the following steps: first select the Phalaenopsis species that will need to be propagated quickly, take pictures with a digital camera, archive and preserve the parental image data, and then perform the following operations: Step 1. Cultivate the Phalaenopsis that needs to be rapidly propagated until the pedicel is drawn out. If the pedicel has been drawn out, cut it directly with a scalpel; Step 2. After sterilizing the cut pedicel with cotton dipped in 75% alcohol, use a sharp scalpel along the pedicel Cut a circular wound on the periphery of the peduncle, then break it off with tweezers, put the folded pedicel segment into a sterilized glass bottle, pour 1.0% sodium hypochlorite solution for disinfection, peel off the bract leaves with a scalpel, and then pour 0.75% Disinfect in sodium hypochlorite solution for 5 minutes, then rinse with sterile water, cut ...

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Abstract

The invention discloses a method for breeding irises by the method of a biological reactor, which adopts the procedures that the first procedure comprises breeding the iris which needs to be quickly bred until a pedicel grows, if a pedicel grows, the pedicel is cut and fetched with a cutter, the second procedure comprises disinfecting the pedicle which is cut and fetched and stripping the bract coat outside of nodal bud, the third procedure comprises cutting young leaves which are cut into small pieces, inducting generation of somatic cell embryo masses of the small pieces which are cut under appropriate hormones combination and an appropriate culture medium, selecting and cutting somatic cell embryo masses in different growing stages into material blocks and gradually dehydrating through butanol, the forth procedure comprises inoculating somatic cell embryo masses after being processed by puncture wound on an appropriate liquid culture medium to quickly proliferate in large quantity, the fifth procedure comprises dropping the liquid culture medium which contains the somatic cell embryo masses on an appropriate differentiation culture medium to induce generation of small plants, the sixth procedure comprises breeding small plants with enough numbers till seedlings are transplanted outside of bottles for bleeding, and the seventh procedure comprises normally managing the small seedlings.

Description

technical field [0001] The invention relates to a method for multiplying moth orchids in large quantities by means of a bioreactor. Background technique [0002] Phalaenopsis (Phalaenopsis) is known as the "Queen of Orchids" and has high ornamental value and commercial value. The traditional propagation method of Phalaenopsis seedlings is branch propagation or rapid propagation by bud growth by tissue culture. The reproduction coefficient is generally only about 2.0. From a new variety to industrialized rapid propagation to a total output of about 100,000 plants, It takes at least four years. Contents of the invention [0003] The invention provides a method for quickly multiplying Phalaenopsis orchids in large quantities through a bioreactor, which can solve the problem that it is difficult for fine varieties to form large-scale production in a relatively short period of time. [0004] The present invention has adopted following technical scheme as follows: a kind of me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李杰
Owner 李杰
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