Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Antisense nucleic acid of resistant and tolerant dimethoxyphenecillin staphylococcus aureus drug resistant gene

A methicillin-resistant, antisense nucleic acid technology, applied in the antisense nucleic acid field of anti-methicillin-resistant Staphylococcus aureus drug resistance gene, can solve the problem of not being a good target site, etc., to achieve the suppression of MRSA drug resistance, The effect of broad application prospects

Inactive Publication Date: 2008-06-18
FOURTH MILITARY MEDICAL UNIVERSITY
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually the lower their negative value, the more stable, not a good target site

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antisense nucleic acid of resistant and tolerant dimethoxyphenecillin staphylococcus aureus drug resistant gene
  • Antisense nucleic acid of resistant and tolerant dimethoxyphenecillin staphylococcus aureus drug resistant gene
  • Antisense nucleic acid of resistant and tolerant dimethoxyphenecillin staphylococcus aureus drug resistant gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The design of antisense thio-oligodeoxynucleotides (PS-ODNs) and thio-deoxyribozyme (PS-DRz) of embodiment 1 anti-mecR1 or blaR1 mRNA:

[0029] The sequence of the drug-resistant gene mecR1 / mecA of MRSA (gene number gi:2791983) was retrieved in GENBANK. The sequence is 9047bP in full length and encodes 8 open reading frames, including 3 mec regions and 5 ORF regions. The sequence between 1615bp and 3372bp is the mecR1 gene sequence, and the sequence between 3472bp and 5478bp is the mecA gene sequence. The full length of mecR1 is 1758bp, which is the upstream gene of mecA, which can encode MecR1 protein and regulate the expression of mecA; the full length of mecA is 2007bp, which encodes the penicillin binding protein PBP2a. Using the same method, the genome sequence of blaR1 / blaZ (gene number gi: 33390917) was retrieved. The sequence is 3080 bp in full length and encodes three open reading frames, namely blaZ, blaR1 and blal. The sequence between 953bp and 2710bp is th...

Embodiment 2

[0034] Design and synthesis of embodiment 2PCR primers:

[0035] According to the gene sequences of mecR1, mecA, blaR1, blaZ and 16srRNA of MRSA reported in GeneBank, five pairs of specific amplification primers for mecR1, mecA, blaR1, blaZ and 16srRNA were designed with the software Primer Premier5.0. The primers were verified to be highly specific by BLAST software, and were synthesized by Shanghai Bioengineering Technology Service Co., Ltd. The sequences are shown in Table 2.

[0036] Table 2 designed and synthesized amplification primers

[0037]

Embodiment 3

[0038] Example 3 Preparation of electrocompetent WHO-2:

[0039] The methicillin-resistant Staphylococcus aureus strain WHO-2 was provided by the China Antimicrobial Resistance Surveillance Center. Transplant 1ml of freshly cultured overnight WHO-2 into 100ml of nutrient broth medium, culture at 37°C and 210rpm until mid-logarithmic growth, and measure its OD 600 =0.55~0.6. After 20 minutes in ice bath, subpackage (40ml / centrifuge tube), centrifuge at 6000rpm for 10min, discard the supernatant, mix and resuspend the bacterial pellet with 40ml ice-bathed deionized water, centrifuge at 6000rpm for 10min, discard the supernatant, repeat 2 times. Afterwards, the bacterial pellet was mixed and resuspended with 20 ml ice-bathed 10% glycerol, centrifuged at 6000 rpm for 10 min, and the supernatant was discarded. The bacterial pellet was mixed and resuspended with 1 ml of ice-bathed 10% glycerol, centrifuged at 6000 rpm for 10 min, the supernatant was discarded, and repeated twice....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to antisense oligonucleotides aiming to methicillin-resistant Staphylococcus aureus (MRSA) resistance gene mecR1 / mecA and blaR1 / blaZ and a preparation of the drugs with the antisense oligonucleotides and the application thereof. The antisense oligonucleotides comprise antisense phosphorothiate oligodeoxynucleotide (PS-ODNs) and thiodeoxyribozyme (PS-DRz). The invention is characterized in that the combination of the specificity and the various areas of MRSA resistance gene mecR1 / mecA and blaR1 / blaZ blocks the expression of the resistance gene, the base sequence of the antisense nucleic acid is PS-ODNs01-40, the antisense nucleic acid can combine with the specific site of the target gene, thereby inhibiting the expression of the resistance gene, causing MRSA to restore the sensitivity of the lactam antibiotics and achieving the purpose of effective fighting with the drug resistance of MRSA.

Description

technical field [0001] The invention relates to a class of antisense nucleic acid sequences for drug-resistant genes mecR1 / mecA and blaR1 / blaZ of methicillin-resistant Staphylococcus aureus (MRSA), preparation of medicines containing the antisense nucleic acid sequences and use thereof. Background technique [0002] At present, the problem of bacterial drug resistance has intensified around the world, and many bacterial infectious diseases that were once curable are becoming incurable diseases. MRSA is an important pathogenic bacterium for nosocomial infection and community infection. Since MRSA was first discovered in 1961, especially after the 1980s, the infection rate of MRSA has shown a clear upward trend in most areas. For example, in the United States, the infection rate of MRSA increased from 1974 to 2% in 2003 to nearly 60% in 2003, an increase of almost 30 times in 30 years; Italy rose from 6% in 1981 to 26% in 1996. With the continuous improvement of MRSA infectio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H21/04C12N15/11A61K48/00C12N15/113
Inventor 罗晓星孟静茹侯征扈本荃王慧贾敏
Owner FOURTH MILITARY MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com