Mechanics biology experimental installation capable of providing mechanics and chemistry signals synchronously

Abstract

The invention provides a mechanical and biologic experimental apparatus to provide mechanical and chemical signals synchronously, and is characterized in that a two-layer fluid chamber, which is a core part, consists of an upper layer, a middle layer and a lower layer, which are combined. The two-layer fluid chamber comprises an upper-layer chamber and a lower-layer chamber. Each of the chambers is provided with an inlet and an outlet. The heads of the inlet and the outlet of the upper layer chamber are T-shaped, and are used to develop entry flow. A lower liquid storage tank is connected with an upper liquid storage tank through a peristaltic pump. An overflow pipe arranged inside the upper liquid storage tank is connected with the lower liquid storage tank so that superfluous liquid directly flows into the lower liquid storage tank through the overflow pipe orifice to keep the liquid level inside the upper liquid storage tank constant. The outlet of the upper liquid storage tank is connected with the inlet of the upper layer chamber. The outlet of the upper layer chamber is connected with the lower liquid storage tank. The inlet and the outlet of the lower layer chamber are connected with a replenishment liquid storage tank to keep the concentration of the liquid inside the lower-layer chamber constant, and to keep the liquid in the lower layer chamber still relative to the fluid inside the upper layer chamber. The two-layer fluid chamber and the upper liquid storage tank are arranged on the upper mounting plate of a metal holder; the replenishment liquid storage tank is arranged on the middle mounting plate of the metal holder; the lower liquid storage tank is arranged on the lower mounting plate of the metal frame. The two-layer fluid chamber, the upper liquid storage tank, the replenishment liquid storage tank and the lower liquid storage tank are connected through pipes and constitute a whole.

Description

1. Technical field [0001] The invention relates to a mechanobiology experimental device that simultaneously provides mechanochemical signals, belongs to the field of mechanobiology, and can simultaneously provide fluid shear stress stimulation and chemical stimulation for cells cultured in vitro, and is used for studying in vitro culture under mechanical-chemical coupling cell response. 2. Background technology [0002] With the development of cell and molecular biology, the study of biomechanics has gone from the level of organs and tissues to the level of cells, molecules and genes, and thus a new interdisciplinary subject, Mechanobiology, has emerged. The three main research contents of mechanobiology are mechanical stimulation signal, signal transduction pathway and cell response to stress. The experimental device of mechanobiology is the foundation of mechanobiology research. As a new interdisciplinary subject, it needs corresponding experimental techniques and devices...

AI Technical Summary

Problems solved by technology

At present, there is still a lack of an effective technical means that can be used to study the response of cells under the combined stimulation of shear stress and chemical factors, and the mechanical conduction pathway between shear stress and chemical factors

[0006] 2. In terms of ensuring the similarity of the flow field, the traditional or improved parallel plate flow chamber cannot meet the following parameters: L (length) ≥ H (height), W (width) ≥ H and L / H > 10 index

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