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Method for extracting phycocyanin from Microcoleus vaginatus

A technology of microcoleans and phycocyanin, which is applied in the field of extracting phycocyanin, can solve the problem of high cost, and achieve the effect of convenient operation, simple equipment, economical and quick

Inactive Publication Date: 2012-01-04
CHINA UNIV OF GEOSCIENCES (WUHAN)
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Problems solved by technology

At present, the blue pigments used in food and other industries are mainly chemically synthesized pigments, while natural blue pigments are rarely used. Most of the phycocyanins are extracted from spirulina and large seaweed, and their phycocyanin content is mostly higher than that of sheaths. Microcoleum is lower. In addition, the cost of cultivating macroalgae is much higher than the cost of cultivating Microcoleella

Method used

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Embodiment Construction

[0012] The specific implementation steps of extracting phycocyanin from Microcoletheca sheaths are as follows:

[0013] A. Preparation of culture medium

[0014] Add 1.5g of sodium nitrate, 0.04g of dipotassium hydrogen phosphate, 0.075g of magnesium sulfate, 0.036g of calcium chloride, 0.006g of citric acid, 0.006g of ferric ammonium citrate, 0.001g of disodium edetate, Sodium carbonate 0.02g, trace element solution 1ml, wherein trace element solution is added boric acid 286mg, manganese chloride tetrahydrate 181mg, zinc sulfate heptahydrate 22.2mg, sodium molybdate dihydrate 25.2mg, copper sulfate pentahydrate 7.9 mg per 100ml of distilled water. mg, completely dissolved and then stirred evenly to prepare the culture medium.

[0015] B. Cultivation of Microsheathing algae

[0016] (1) Primary culture: insert Microcoletheca species into a triangular flask (150-200ml) filled with culture medium, culture statically or with aeration, and adjust the airflow to 2.5L.min during a...

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Abstract

The invention discloses a method for extracting a phycocyanin from a microcoleus vaginatus, which comprises the following steps: preparing the microcoleus vaginatus culture medium with a sodium nitrate, a potassium phosphate dibasic, a magnesium sulfate, a calcium chloride, a citric acid, a ferric ammonium citrate, an EDTA, a sodium carbonate and a trace element solution according to a certain proportion, culturing the microcoleus vaginatus in the culture medium and making the microcoleus vaginatus powder, extracting a phycocyanin from the microcoleus vaginatus powder and purifying the phycocyanin. The method for extracting a phycocyanin from a microcoleus vaginatus has the advantages of simplicity, convenient operation, safety and reliability, high yield, good product quality and simple operated equipment, which is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting phycocyanin from Microcoletheca sheaths, belonging to the field of biotechnology. Background technique [0002] Microsheathing algae belongs to Cyanophyta, Cyanophyta, Seglophytes, Oscillatoriaceae, Microcoleum genus. It is the dominant population in biological crusts in arid and semi-arid regions. At present, the utilization of Microcoleans generally only stays in artificial algae crusts. The reason why Microcoleans is used to form artificial algal crusts for desert control is mainly because it is rich in phycocyanin. Phycobiliproteins are a class of pigment complex proteins present in the phycobilisomes of some algae (mainly red and blue algae). Phycobiliprotein has strong fluorescence and emits orange-red fluorescence, but itself is red, purple or blue, so it is a colored polypeptide. According to the spectral characteristics, phycobiliproteins can be divided into four categories: phycoerythrin, ph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/14
Inventor 谢作明刘永定王焰新沈银武胡春香
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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