MR gene order of diffuse large B cell lymphoma related antigen specific TCR V alpha 6 subfamily and uses thereof

A gene sequence, lymphoma technology, applied in the direction of receptor/cell surface antigen/cell surface determinant, application, gene therapy, etc., can solve the problem of understanding antigen

Inactive Publication Date: 2008-07-23
JINAN UNIVERSITY +1
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on lymphoma at home and abroad mainly focuses on T-c

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • MR gene order of diffuse large B cell lymphoma related antigen specific TCR V alpha 6 subfamily and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] Example

[0034] (1) Analysis of antigen-specific TCR gene sequences

[0035]The method for analyzing the length and base sequence of CDR3 of TCR Vα gene profile is to analyze the length of CDR3 of TCR Vα 29 subfamilies by RT-PCR-gene scanning method. First, the peripheral blood of DLBL patients was collected, and the peripheral blood mononuclear cells were separated by Ficoll stratification. 7 RNA was extracted from PBMCs (peripheral blood mononuclear cells), and cDNA was synthesized by RT-PCR. At the same time, the gene sequence of TCRα chain was found in the gene bank of NCBI, and the positions of V region and C region of each subfamily in the genome were marked. 29 Vα-specific upstream primers were designed according to the cDNAs of the 29 Vα subfamily genes, and a Cα downstream primer was set in the Cα region. PCR technology was used to amplify the V-N-JC region generated by the TCR rearrangement of the 29 Vα subfamily genes of the patient. . As shown in Figure...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a nucleotide sequence and the application of an antigen-specific T cell receptor of the diffusive large B cell lymphoma. According to the RT-PCR genescan analysis, the TCR V Alpha 6 subfamilies in the peripheral blood of patients shows an obviously monoclonal state, and the V-N-J region, namely, the complementary determining region 3, where the PCR products conduct the sequence analysis of the specific TCR sequence, is a part recognizably bound to the antigenic specificity; the genescan analysis also shows that the TCR of the clonal T cell is a specific TCR produced due to the stimulus of the DLBL associated antigens, and is the basis and key for performing DLBL molecular targeted adoptive immunotherapy by means of the TCR transgenic technique. The nucleotide sequence of the specific TCR provides a significant basis for further carrying out lymphoma-specific cellular immunotherapy.

Description

technical field [0001] The invention relates to a T cell receptor (TCR) nucleotide sequence, in particular to the MR gene sequence and application of diffuse large B cell lymphoma (DLBL) related antigen-specific TCR Vα6 subfamily. Background technique [0002] In recent years, the incidence of non-Hodgkin's lymphoma (NHL) has continued to rise. It is estimated that NHL will account for 4.4% of aggressive malignancies and 4.8% of all cancer deaths in the early 21st century. Among them, diffuse large B-cell lymphoma (DLBL) is the most common NHL, accounting for about 30% to 40%, and elderly patients over 60 years old account for 70%. For more than 20 years, CHOP regimen (cyclophosphamide, doxorubicin, vincristine, prednisone) was mainly used as the main chemotherapy. However, it has toxic effects on some elderly people, and the long-term survival rate is low. Although the recent emergence of targeted immunotherapy such as rituximab (CD20 monoclonal antibody) has achieved ne...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/12C12N15/10C07K14/725A61K31/7088A61K48/00A61P37/02
Inventor 李扬秋谭获尹青松杨力建陈少华叶静梅吴秀丽
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap