Method for preparing phosphatidyl tocopherol

A kind of technology of phosphatidyl tocopherol and tocopherol, applied in the field of preparation of phosphatidyl tocopherol

Inactive Publication Date: 2008-07-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through literature search, there is no report on the method of preparing phosphatidyl tocopherol without lysophospholipid and phosphatidic acid from soybean mixed phospholipids as raw material and using anhydrous system treated peanut phospholipase D enzyme method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Add 50 g of mixed phospholipids into 1000 ml of chloroform and dissolve at 20°C, add 100 g of tocopherol and stir at constant temperature for half an hour, add 5 g of processed peanut phospholipase D, stir and react at 50°C for 24 hours, and then use 3000 ml of deionized water Wash the organic phase three times, add 10 grams of anhydrous sodium sulfate to the organic phase for drying, and after vacuum precipitation of the dried organic phase, fully wash with 500 ml of acetone, separate the acetone insoluble matter, and dry to obtain phosphatidyl tocopherol 55.8% lysophospholipid and phosphatidic acid free product.

Embodiment 2

[0015] Add 50 grams of mixed phospholipids into 1000 ml of ether and dissolve at 20°C, add 100 g of tocopherol and stir at constant temperature for half an hour, add 5 g of treated peanut phospholipase D, stir and react at 20°C for 48 hours, and use 3000 ml of deionized water Wash the organic phase three times, add 10 grams of anhydrous sodium sulfate to the organic phase for drying, and after vacuum precipitation of the dried organic phase, fully wash with 500 ml of acetone, separate the acetone insoluble matter, and dry to obtain phosphatidyl tocopherol 56.3% lysophospholipid and phosphatidic acid free product.

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Abstract

The invention relates to a preparation method for phosphatidy tocopherol, belonging to enzymatic modification technique of phospholipids, which is characterized in that soybean mixed phospholipids is dissolved in waterless organic solvent, and added with tocopherol, and then processing base exchange reaction under the catalysis of treated peanut phospholipase, which converts the mixed phospholipids into phosphatidy tocopherol. The acetone insoluble matter is separated from the reactant via water-washing the organic part, washing by acetone after dewatering and exsolution, and the product is obtained after drying which contains more than 55 percent of phosphatidy tocopherol by HPLC determination. The preparation method excludes undesired components such as lysophosphatide and phosphatidic acid in phosphatidy tocopherol product since no water exists, and can prepare phosphatidy tocopherol conveniently which has a wide use in medical industry and liposome industry.

Description

technical field [0001] A preparation method of phosphatidyl tocopherol belongs to the technical field of enzymatic modification of phospholipids. Background technique [0002] Phospholipids mainly include phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylpropanol and a series of phospholipid derivatives. Recent studies have found that phosphatidyl tocopherol has important biological functions and has been widely used in the pharmaceutical industry, but phosphatidyl tocopherol hardly exists in nature. Therefore, it is of great significance to prepare phosphatidyl tocopherol by enzymatic method. Through literature search, there is no report on the method of preparing phosphatidyl tocopherol without lysophospholipids and phosphatidic acid from soybean mixed phospholipids as raw material and using peanut phospholipase D enzyme method treated in anhydrous system. Contents of the invention [0003] The object of the present in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06
Inventor 曹栋史苏佳张永刚
Owner JIANGNAN UNIV
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