Sugar cane stem specific expression promoter and plant expression vector thereof

Abstract

The invention relates to a special and high-effective expression promoter of a sugar cane stem and an expression vector of a plant, a PCR method is enlarged after the total DNA is extracted from sugar cane, and then the expression vector is connected with a pMD-18T vector, the cell of bacillus coli DH5Alpha is transformed, through selecting the masculine, the promoter of the sugar cane hexose transportation protein gene PST2(Alpha) is cloned and obtained, the length of the promoter is 1968bp, through replacing the composition CaMV35S promoter on the expression vector pCAMBIA1301 of the plant, a new expression vector of the plant is constructed, and the name is pCAMBIA1900. By obtaining the promoter, an effective tool is provided for the research of the sugar cane trans-gene and the sugar cane molecular in the biology, and the essential condition of the research and the development of the biological product with high additional archery is particularly created for adopting the sugar cane as the bioreactor production. Therefore, the obtaining of the promoter has an important theoretical and practice significance.

Description

technical field [0001] The present invention relates to the fields of plant molecular biology and plant genetic engineering, in particular, it is about cloning a gene promoter sequence from sugarcane genomic DNA, and constructing a sugarcane stem-specific gene containing GUS gene and other target genes downstream of the promoter. High-efficiency expression vector. Background technique [0002] Sugarcane is a plant of the Graminaceae genus Saccharum L., native to tropical and subtropical regions. Sugarcane is a C4 plant with high light efficiency, high light saturation point, low carbon dioxide compensation point, low photorespiration rate, and high photosynthetic intensity. Therefore, sugarcane has high biological yield and large profits. Sugarcane is an ideal material for bioreactors, and its advantages mainly include the following aspects: 1. Easy to plant: Sugarcane likes light and heat, and is the fastest growing plant in tropical and subtropical regions. Multiple harv...

AI Technical Summary

Problems solved by technology

Although these promoters can initiate the high-efficiency expression of exogenous genes in plants, the high-efficiency expression of exogenous genes, especially the all-round expression in the whole plant, will inevitably consume a large amount of basic materials in the plant, and the additional consumption of basic materials will inevitably Influence and inhibit other metabolic activities in plants, which not only causes waste of exogenous genes but also causes changes in plant morphology, thus directly affecting plant growth and development

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