Sugar cane stem specific expression promoter and plant expression vector thereof

A plant expression vector and expression vector technology, applied in the field of specific and high-efficiency expression vectors for sugarcane stems, can solve the problems of plant morphological changes, waste of exogenous genes, mass consumption, etc.

Inactive Publication Date: 2008-10-08
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these promoters can initiate the high-efficiency expression of exogenous genes in plants, the high-efficiency expression of exogenous genes, especially the all-round expression in the whole plant, will inevitably consume a large amount of basic materials in the pla

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sugar cane stem specific expression promoter and plant expression vector thereof
  • Sugar cane stem specific expression promoter and plant expression vector thereof
  • Sugar cane stem specific expression promoter and plant expression vector thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] The present invention will be further described below.

[0032] 1. Extraction of Genomic DNA from Sugarcane Leaves

[0033] Take about 1 g of sugarcane leaves, grind them into powder with liquid nitrogen, add 2 ml of preheated 2% CTAB extraction buffer, incubate at 65°C for 1 hour; centrifuge at 4°C, 5000r / min for 2-3 minutes, transfer the supernatant To another centrifuge tube, add an equal volume of chloroform:isoamyl alcohol (24:1), and centrifuge at 10,000r / min for 10 minutes; take the supernatant and add an equal volume of isopropanol to precipitate, and place it at room temperature for 30min; centrifuge at 12,000r / min for 10min , washed twice with 70% alcohol, dried in the air, dissolved in TE buffer with RNAse, incubated at 37°C for 30 minutes; added an equal volume of phenol: chloroform: isoamyl alcohol (25:24:1), and gently inverted , mix well, and centrifuge at 12000r / min for 10min; take the supernatant and add phenol: chloroform: isoamyl alcohol to repeat th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a special and high-effective expression promoter of a sugar cane stem and an expression vector of a plant, a PCR method is enlarged after the total DNA is extracted from sugar cane, and then the expression vector is connected with a pMD-18T vector, the cell of bacillus coli DH5Alpha is transformed, through selecting the masculine, the promoter of the sugar cane hexose transportation protein gene PST2(Alpha) is cloned and obtained, the length of the promoter is 1968bp, through replacing the composition CaMV35S promoter on the expression vector pCAMBIA1301 of the plant, a new expression vector of the plant is constructed, and the name is pCAMBIA1900. By obtaining the promoter, an effective tool is provided for the research of the sugar cane trans-gene and the sugar cane molecular in the biology, and the essential condition of the research and the development of the biological product with high additional archery is particularly created for adopting the sugar cane as the bioreactor production. Therefore, the obtaining of the promoter has an important theoretical and practice significance.

Description

technical field [0001] The present invention relates to the fields of plant molecular biology and plant genetic engineering, in particular, it is about cloning a gene promoter sequence from sugarcane genomic DNA, and constructing a sugarcane stem-specific gene containing GUS gene and other target genes downstream of the promoter. High-efficiency expression vector. Background technique [0002] Sugarcane is a plant of the Graminaceae genus Saccharum L., native to tropical and subtropical regions. Sugarcane is a C4 plant with high light efficiency, high light saturation point, low carbon dioxide compensation point, low photorespiration rate, and high photosynthetic intensity. Therefore, sugarcane has high biological yield and large profits. Sugarcane is an ideal material for bioreactors, and its advantages mainly include the following aspects: 1. Easy to plant: Sugarcane likes light and heat, and is the fastest growing plant in tropical and subtropical regions. Multiple harv...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/29C12N15/82
Inventor 张树珍王正鹏杨本鹏蔡文伟冯翠莲顾丽红
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap