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Fluorescence quantification detection method of hepatitis B virus cccDNA and kit

A fluorescent quantitative detection and hepatitis B virus technology, applied in the field of genetic engineering, can solve the problem of low sensitivity, and achieve the effects of broad market prospects, good specificity, and good clinical application value.

Inactive Publication Date: 2008-10-15
NANJING ZHONGXIN TRANSLATIONAL MEDICINE INSTCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The present inventor has been engaged in the research of above-mentioned method, once published the article of cccDNA quantitative detection method, but the sensitivity of this method is not very high, only has 10 3 -10 9 copy / ml, and the required amount of sequence sample DNA is 10ng / ml

Method used

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  • Fluorescence quantification detection method of hepatitis B virus cccDNA and kit
  • Fluorescence quantification detection method of hepatitis B virus cccDNA and kit
  • Fluorescence quantification detection method of hepatitis B virus cccDNA and kit

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Embodiment 1

[0031] Step 1. Collect samples

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Abstract

The invention relates to a fluorescent quantitative detection method and a reagent kit for a hepatitis B virus cccDNA, which belong to the genetic engineering field. The fluorescent quantitative detection method for the hepatitis B virus cccDNA comprises a PCR augmentation reaction system and is characterized in that: the PCR system comprises an upstream primer, a downstream primer and a probe, wherein, the upstream primer is positioned at a HBV genome nucleotide sequence 1520-1580, the downstream primer is positioned at a HBV genome nucleotide sequence 1920-2000; and the probe is positioned at a HBV genome nucleotide sequence 1850-1900, and both ends of the probe are respectively connected with a fluorescent group and a quenching group. The method has high sensitivity and a broad linear detection range, and can save the consumption of reagent.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a hepatitis B virus cccDNA fluorescence quantitative detection method and a kit. Background technique [0002] Hepatitis B virus (HBV) infection can cause chronic hepatitis, liver cirrhosis, and even liver cancer. There are about 120 million HBV-infected people in my country, and about 35 million patients with chronic hepatitis B (chronic hepatitis B), of which about 20% of patients will develop liver cirrhosis, and about 5-10% of patients will develop liver cancer. At present, there are no effective drugs and methods to cure chronic hepatitis B at home and abroad. Therefore, chronic hepatitis and liver cirrhosis caused by HBV infection not only seriously threaten the physical and mental health of our people, but also bring huge economic burdens. Studying HBV and finding a treatment method that can completely treat chronic hepatitis B has always been a major research topic in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
Inventor 陆海英
Owner NANJING ZHONGXIN TRANSLATIONAL MEDICINE INSTCO
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