Method for obtaining extract from several frequently seen plants and uses of the extract

A plant extract and extract technology are applied in the directions of plant raw materials, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., and can solve the problems of high cost of cetriol and difficulty in industrialized production, etc. To achieve the effect of preventing and treating diabetes, easy to obtain and simple preparation process

Inactive Publication Date: 2008-12-03
XIANGBEI WELMAN PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2002, Professor Liang Jingyu isolated the extract containing cetriol from yew, which has the effect of treating diabetes (Liang Jingyu, et al., CN14883...

Method used

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  • Method for obtaining extract from several frequently seen plants and uses of the extract
  • Method for obtaining extract from several frequently seen plants and uses of the extract
  • Method for obtaining extract from several frequently seen plants and uses of the extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Preparation 1 of low-purity plant extract

[0027] Plant coarse powder 100kg, reflux extraction with 3 times volume of 90% ethanol three times, combine extracts, concentrate under reduced pressure, use ethyl acetate / water two-phase extraction of 1: 2 (volume / volume), combine water layer, filter, Macroporous resin column (D101 type, domestic) chromatography, gradient elution with distilled water and hydrous ethanol, collect the part containing cetraylol, concentrate and dry under reduced pressure to obtain plant extract (light yellow powder), in which cetraylol The content is 10.1%.

Embodiment 2

[0028] Embodiment 2: the preparation 1 of plant extract of lower purity

[0029] Plant coarse powder 50kg, reflux extraction three times with 90% ethanol 60 ℃ of 3 times of volumes, combine extracts, concentrate to dryness under reduced pressure, use the dichloromethane / water two-phase extraction of 1:1 (volume / volume), combine water layer , filtered, macroporous resin column (D101 type, domestic) column chromatography, gradient elution with distilled water, hydrous ethanol (ethanol: 5%-30%), and each eluent is identified with differential test solution respectively, and the combined positive The eluate from the reaction was concentrated under reduced pressure and dried at 70° C. to obtain a plant extract (slightly yellow powder), in which the content of cetriol reached 27.6%.

Embodiment 3

[0030] Embodiment 3: the preparation 2 of plant extract of lower purity

[0031] Plant meal 60kg, 3 times volume 90% methanol 50 ℃ reflux extraction three times, combine extracts, concentrate to dryness under reduced pressure, use ethyl acetate / water two-phase extraction of 1:1 (volume / volume), combine water layer , filtered, macroporous resin column (MN-200 type, domestic) column chromatography, gradient elution with distilled water, hydrous ethanol (ethanol: 5%-20%), each eluent is identified with differential test solution respectively, merges The eluate showing a positive reaction was concentrated under reduced pressure and dried at 70° C. to obtain a plant extract (slightly yellow powder), wherein the content of cetriol reached 28.2%.

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PUM

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Abstract

The invention relates to a method for preparing a sequoyitol-containing extract extracted from a plurality kinds of plants and an application of the extract in preventing and treating diabetes. The method comprises the following steps that: plants of trifolium, plants of leguminosae and plants of ginkgoaceae are used as raw materials and are extracted by methanol, alcohol or acetone to give extracts. The extracts undergo two-phrase extraction of water and chloroform or ethyl acetate or methylene chloride or n-butylalcohol. The solvent is reclaimed and the water part is separated and purified by macro porousresin to obtain the extract which is white powder. The main ingredient of the extract is sequoyitol with a content of between 10 and 99.9 percent. The extract obtained by the method and drugs, functional food or health care food made from the extract can be used for the treatment of diabetes and complications of diabetes.

Description

field of invention [0001] The invention relates to a method for obtaining an extract mainly containing cetrayiol from plants, and the use of the extract; the extract can be used for treating diabetes. Common plants include Trifolium plants - red clover (T.pratense), white clover (T.repens), crimson clover (T.incarnatum), Egyptian clover (T.alexandrinum) Trifolium pratense (T.campestre), Trifolium obtuseleaf (T.dubium), Trifolium grandiflora (T.eximium), Trifolium fragrans (T.fragiferum), Trifolium fragrans (T.gordejevi), Hybrid clover Grass (T.hybridum), White-flowered Wildfireball (variety) (T.lupinaster var.albiflorum), Wildfireball (original variety) (T.lupinaster var.lupinaster), Middle Trifolium (T.medium), Yellow Trifolium ( T.strepens), wildfire ball (T.lupinaster) etc.; said soybean (Leguminosae) plants include soybean (Glycine max (Linn.) Merr.), broad leaf vine bean (original variety) (Glycinegracilis Skv.Var.gracilis), Penghu Soybean (Glycine clandestine Wendl.), ...

Claims

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Application Information

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IPC IPC(8): A61K36/16A61K36/48A61P3/10A61P3/06A61P13/12A61P9/00A61P9/14A61P25/00C07C43/196
CPCA61K36/48A61K36/16A61P3/06A61P3/10A61P9/00A61P9/10A61P9/14A61P13/00A61P13/12A61P25/00A61P27/02A61K2300/00
Inventor 孙天宇王艳丽邓桂兴王音音陆游王霆
Owner XIANGBEI WELMAN PHARMA CO LTD
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