Nano liposome anti-HPV, gynecological inflammation pathogen specific compound IgY and combined preparation thereof
An anti-human papilloma, nano-liposome technology, applied in the medical and health field, can solve the problem of destroying the ecological balance of the flora
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experiment example 1
[0095] Detection of IgY content in two kinds of anti-HPV specific IgY and anti-gynecological inflammatory pathogen specific IgY
[0096] Using SDS-PAGE (sodium dodecyl sulfate-polypropylene gel) electrophoresis assay, the two kinds of anti-HPV specific IgY and anti-gynecological inflammatory pathogen specific IgY crude extracts prepared by the above process were detected respectively. , The result contained IgY45-52%. The three kinds of IgY crude extracts were purified by column twice to obtain pure IgY. After SDS-PAGE analysis, the purity reached PAGE pure, as shown in the table below:
[0097] Pure IgY
experiment example 2
[0099] Detection of antibody binding titers of two kinds of anti-human papillomavirus (HPV) and anti-gynecological inflammatory pathogen-specific composite IgY liposomes to six representative HPV viruses
[0100] Two kinds of anti-HPV-specific IgY and anti-HPV-specific IgY liposomes were prepared by using two kinds of immunization antigen schemes of human papilloma cells, viral protein composite antigen and HPVL1, L2 gene expression protein antigen respectively, with 6, 11 , 16, 18, 31 and 45 types of six types of HPV viruses are used as detection antigens, and the two kinds of anti-human papillomavirus (HPV) and anti-gynecological inflammatory pathogens specific composite IgY liposomes are detected by "ELISA" method antibody titer. The results are as follows:
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[0102] As can be seen from the above test results, no matter adopting papilloma cells, virus protein complex antigen or adopting HPV virus L1, L2 gene expression protein antigen to immunize egg-laying poul...
experiment example 3
[0104] The antibody binding titers of two kinds of anti-human papillomavirus (HPV) and anti-gynecological inflammatory pathogen specific complex IgY liposomes to other types of HPV viruses that were not immunized as antigens were detected.
[0105] Two kinds of anti-human papillomavirus (HPV) and anti-gynecological inflammatory pathogen specific composite IgY liposomes prepared by immunization with "human papilloma cells, virus protein composite antigen" and HPV virus L1, L2 gene expression protein antigen respectively, Using HPV types 33, 35, 39, 51, 52 and 58, 56 that are not used as antigens for immunization as detection antigens, use the "ELISA" method to detect the two anti-human papillomavirus (HPV) and anti-gynecological Antibody titers of inflammatory pathogen-specific complexed IgY liposomes. The results are as follows:
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[0107]
[0108] As can be seen from the above test results, no matter adopting human papilloma cells, virus protein composite antige...
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