Nano liposome anti-HPV, gynecological inflammation pathogen specific compound IgY and combined preparation thereof
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An anti-human papilloma, nano-liposome technology, applied in the medical and health field, can solve the problem of destroying the ecological balance of the flora
Active Publication Date: 2008-12-24
SHENZHEN JASON INTELLIGENT BIOTECH CO LIMLTED PRC
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However, when people take antibiotics or clean the vagina with ordinary lotions, bactericidal effervescent tablets, and suppositories, the beneficial bacteria such as Lactobacillus in the vagina will also be killed; thus, the flora that acts as a natural barrier in the vagina will be destroyed Ecological balance causes various pathogens to take advantage of the opportunity to multiply in large numbers; as a result, many women suffer from various gynecological infections and HPV virus infection for life because there is no cure
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experiment example 1
[0095] Detection of IgY content in two kinds of anti-HPV specific IgY and anti-gynecological inflammatory pathogen specific IgY
[0096] Using SDS-PAGE (sodium dodecyl sulfate-polypropylene gel) electrophoresis assay, the two kinds of anti-HPV specific IgY and anti-gynecological inflammatory pathogen specific IgY crude extracts prepared by the above process were detected respectively. , The result contained IgY45-52%. The three kinds of IgY crude extracts were purified by column twice to obtain pure IgY. After SDS-PAGE analysis, the purity reached PAGE pure, as shown in the table below:
[0097] Pure IgY
experiment example 2
[0099] Detection of antibody binding titers of two kinds of anti-human papillomavirus (HPV) and anti-gynecological inflammatory pathogen-specific composite IgY liposomes to six representative HPV viruses
[0100] Two kinds of anti-HPV-specific IgY and anti-HPV-specific IgY liposomes were prepared by using two kinds of immunization antigen schemes of human papilloma cells, viral protein composite antigen and HPVL1, L2 gene expression protein antigen respectively, with 6, 11 , 16, 18, 31 and 45 types of six types of HPV viruses are used as detection antigens, and the two kinds of anti-human papillomavirus (HPV) and anti-gynecological inflammatory pathogens specific composite IgY liposomes are detected by "ELISA" method antibody titer. The results are as follows:
[0101]
[0102] As can be seen from the above test results, no matter adopting papilloma cells, virus protein complex antigen or adopting HPV virus L1, L2 gene expression protein antigen to immunize egg-laying poul...
experiment example 3
[0104] The antibody binding titers of two kinds of anti-human papillomavirus (HPV) and anti-gynecological inflammatory pathogen specific complex IgY liposomes to other types of HPV viruses that were not immunized as antigens were detected.
[0105] Two kinds of anti-human papillomavirus (HPV) and anti-gynecological inflammatory pathogen specific composite IgY liposomes prepared by immunization with "human papilloma cells, virus protein composite antigen" and HPV virus L1, L2 gene expression protein antigen respectively, Using HPV types 33, 35, 39, 51, 52 and 58, 56 that are not used as antigens for immunization as detection antigens, use the "ELISA" method to detect the two anti-human papillomavirus (HPV) and anti-gynecological Antibody titers of inflammatory pathogen-specific complexed IgY liposomes. The results are as follows:
[0106]
[0107]
[0108] As can be seen from the above test results, no matter adopting human papilloma cells, virus protein composite antige...
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Abstract
The invention relates to nanoliposome human papillomavirus (HPV) resistant and gynecological inflammation pathogen resistant specific complex IgY and a combined preparation for the same. Laying poultry is preferred to respectively prepare laying poultry for immunity of tumor somatic cells, viral protein complex antigens, gene recombination protein antigens and bacterial virus complex antigens; and human papillomavirus resistant special IgY and gynecological inflammation pathogen resistant specific IgY undergo affinity chromatography and purification and are converted into nanoliposomes, and then the nanoliposome special complex IgY is prepared. The nanoliposome human papillomavirus (HPV) resistant and gynecological inflammation pathogen resistant specific complex IgY and the combined preparation for the same have the advantages that: the nanoliposome technology is applied to increase the infiltration capacity and the prolongation effect and improve the curative effect. The nanoliposome human papillomavirus (HPV) resistant and gynecological inflammation pathogen resistant specific complex IgY and the combined preparation for the same can be prepared into various vaginal spraying agents, other spraying agents, lotion, gel, capsules, suppository, vaginal membranes, tablets, effervescent tablets, ointment, cream and so on. The nanoliposome complex IgY can infiltrate into a vagina mucosa to effectively remove HPV viruses and gynecological inflammation pathogens which are absorbed on the vaginal surface layer and in the vagina mucosa, is absorbed through the vagina mucosa and enters into the body to give play to the functions of prevention and treatment, and also can effectively prevent recrudescence.
Description
technical field [0001] The invention relates to the field of medical and health technology, in particular to a nanoliposome anti-human papillomavirus (HPV) and anti-gynecological inflammation pathogen specific composite IgY and a combined preparation containing the nanoliposome composite IgY. Background technique [0002] At present, cervical cancer is a disease that seriously endangers women's health and life. There are about 500,000 new cases and 250,000 deaths every year. It is the second leading cause of women's death, most of which are in developing countries, accounting for about 80%. On the basis of a large number of experimental studies, modern medicine has confirmed that cervical cancer is derived from a human papillomavirus (HPV), which is the only cancer with a clear etiology among all human cancers. [0003] A large number of testing data show that HPV infection is most common among young people between the ages of 18 and 28. It can be said that more than 50% of...
Claims
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