Method for producing vindoline

Abstract

The invention discloses a method for producing vindoline. The method comprises: inoculating a periwinkle polyploid cell strain in a synthetic medium, and obtaining a cell rich in vindoline by cultivating the strain for 3 to 7 days at the temperature between 20 and 28DEG C; and the synthetic medium is a liquid culture medium obtained by adding 0 to 4mg / L of plant cell auxin, 0 to 4mg / L of plant cytokinin, 30 to 60g / L of sucrose, 10 to 40mu g / L of acetyl coenzyme A, 0.5 to 1.5mu mol / L of Benzotriazole methl, 5 to 30mu mol / L of tranylcypromine, 10 to 40mu l / L of acetic anhydride and 10 to 40mg / Lof dithiothreitol in a minimal medium. The method using periwinkle cells to cultivate and produce vindoline can stably realize the industrialized production of vindoline, and the production cost is rather low, the culture period is short, and the production is insusceptible to the natural environment and weather, so the year-round production can be realized.

Description

technical field

[0001] The invention relates to a method for producing ventolin, in particular to a method for producing ventolin by using large-scale cultivation of plant cells and metabolic regulation technology. Background technique

[0002] Periwinkle (Catharanthus roseus (L) G. Don) is a genus with rich alkaloid content in Apocynaceae. Vinca alkaloids mainly include: Aspicospermatan type such as Vindoline (Vindoline) etc., Ibogan type such as Vinblastine (Catharanthine) etc., Corynanthean type such as Ajmalicine (Ajmalicine) and Serpentine (Serpentine) etc. and Bisindoles type such as Vinblastine and Vincristine etc. In the mid-1950s, some scholars found that the extract of periwinkle plants also had the effect of lowering white blood cells when studying the hypoglycemic effect of periwinkle plants. In 1958, Canadian Noble and Beer jointly isolated this active ingredient and named it Vinblastine. (vinblastine) and prepared its sulfate. Subsequently, Lilly Company of ...