Method for promoting biological detection sensitivity by photon crystal

A technology of photonic crystals and biological detection, which is applied in the direction of biological testing, chemiluminescence/bioluminescence, measuring devices, etc., can solve the problems of restricting the pace of practical application, expensive instruments, and difficulties, so as to improve stability and sensitivity, and make The effect of low cost and improved stability

Active Publication Date: 2009-01-07
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the perspective of improving the instrument, in order to meet the high-sensitivity test requirements, it is necessary to strictly control the experimental conditions, minimize the background fluorescence, use a complex optical system, and have particularly high requirements for the quality of the detector, so the instrument is expensive. The experimental process has strict requirements, and it is difficult to popularize and apply this method, which seriously restricts the pace of its practical application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Prepare a solution of poly(styrene-methyl methacrylate-acrylic acid) latex particles with a mass concentration of 0.2% and a monodisperse particle size of 210 nm with water, and fully sonicate the solution to make the latex particles uniformly dispersed.

[0040] 2. Using a clean carrier as the substrate, under the constant conditions of a temperature of 50°C and a humidity of 50%, the latex solution obtained in step 1 is deposited on the substrate by the vertical deposition method, and the self-assembly arrangement is prepared on the substrate A poly(styrene-methyl methacrylate-acrylic acid) photonic crystal is obtained. The poly(styrene-methyl methacrylate-acrylic acid) photonic crystal is an opal structure with a periodic dielectric structure of the photonic band gap. The photonic band gap lies between 518~527nm.

[0041] 3. The concentration of water is 10 -5 mol / L ethidium bromide (EB) solution.

[0042] 4. Drop the fluorescein (F1)-labeled DNA probe molecule soluti...

Embodiment 2

[0047] 1. Prepare a solution of poly(styrene-methyl methacrylate-acrylic acid) latex particles with a mass concentration of 0.2% and a monodisperse particle size of 255nm with water, and fully ultrasonicate to make the latex particles uniformly dispersed.

[0048] 2. Using a clean carrier as the substrate, under the constant conditions of a temperature of 50°C and a humidity of 50%, the latex solution obtained in step 1 is deposited on the substrate by the vertical deposition method, and the self-assembly arrangement is prepared on the substrate A poly(styrene-methyl methacrylate-acrylic acid) photonic crystal is obtained. The poly(styrene-methyl methacrylate-acrylic acid) photonic crystal is an opal structure with a periodic dielectric structure with a photonic band gap.

[0049] 3. Use commercially available single-stranded DNA with amino end group as the probe molecule, and add EB to the phosphate buffer solution of pH=7.4 of the single-stranded DNA probe molecule with amino en...

Embodiment 3

[0054] 1. Prepare a solution of polymethyl methacrylate latex particles with a mass concentration of 0.2% and a monodisperse particle size of 354 nm with water, and fully sonicate it to uniformly disperse the latex particles.

[0055] 2. Using a clean carrier as the substrate, under constant conditions of temperature 80℃ and humidity 80%, deposit the latex obtained in step 1 on the substrate by vertical deposition method, and prepare by self-assembly on the substrate. The polymethyl methacrylate photonic crystal is obtained. The polymethyl methacrylate photonic crystal is an opal structure with a periodic dielectric structure with a photonic band gap.

[0056] 3. Using ethanol as solvent and concentrated hydrochloric acid as catalyst to hydrolyze tetraethylorthosilicate and improve to prepare SiO 2 Sol. The specific method is as follows: 3.5ml of 28wt% tetraethylorthosilicate, 10ml of absolute ethanol are mixed, and then a catalytic amount of 37wt% of concentrated hydrochloric aci...

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Abstract

The invention relates to a detection method of a variety of proteins and genes in a biological sample, in particular to a method which uses a photonic crystal to improve the sensitivity of biological detection. The method makes use of the characteristics of the photonic crystal to match the fluorescence labeled biological functional matter; the fluorescence labeled biological functional matter is used for detecting the specific response or specific affinity of the target biological matter; the target biological matter responds to the biological functional matter, to result in the luminous change of the biological functional matter which is labeled by fluorescence labeled molecules; a photon crystal is used for enhancing the reading and identification of fluorescence signals of the fluorescence labeled molecules; thus the method can effectively enhance the sensitivity of the detection method of the target biological matter, and further realize the high-sensitivity biological detection.

Description

Technical field [0001] The invention belongs to a method for detecting multiple proteins and genes in biological samples, and particularly relates to a method for improving the sensitivity of biological detection by using photonic crystals. Background technique [0002] The rapid development of life sciences has put forward a large number of new topics in analytical chemistry, currently focusing on the analysis of peptides, proteins, nucleic acids and other biological macromolecules, biopharmaceutical analysis, ultra-trace, ultra-trace biological active substance analysis, and even microbial analysis. Therefore, biological analysis has become one of the most important frontiers in the development of modern analytical chemistry. In order to meet the requirements of this situation, many analytical chemists are constantly striving to develop new analytical methods and techniques. Biosensor is a new type of analytical instrument developed in the context of multidisciplinary cross-dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N21/01G01N33/50
Inventor 李明珠宋延林
Owner INST OF CHEM CHINESE ACAD OF SCI
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