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Method for promoting biological detection sensitivity by photon crystal

A photonic crystal and biological detection technology, which is applied in biological testing, chemiluminescence/bioluminescence, measuring devices, etc., can solve the problems of restricting the pace of practical application, expensive and complicated instruments, and improve stability and sensitivity. Low cost, good selective effect

Active Publication Date: 2011-07-27
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the perspective of improving the instrument, in order to meet the high-sensitivity test requirements, it is necessary to strictly control the experimental conditions, minimize the background fluorescence, use a complex optical system, and have particularly high requirements for the quality of the detector, so the instrument is expensive. The experimental process has strict requirements, and it is difficult to popularize and apply this method, which seriously restricts the pace of its practical application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Prepare a solution of poly(styrene-methyl methacrylate-acrylic acid) latex particles with a monodisperse particle size of 0.2% and a poly(styrene-methyl methacrylate-acrylic acid) latex particle solution with a mass concentration of 0.2% in water, and sufficiently ultrasonicate to make the latex particles uniformly dispersed.

[0040] 2. With a clean carrier film as the substrate, under the constant conditions of temperature of 50°C and humidity of 50%, deposit the latex solution obtained in step 1 on the substrate by vertical deposition method, and prepare by self-assembly and arrangement on the substrate Obtain poly(styrene-methyl methacrylate-acrylic acid) photonic crystal, poly(styrene-methyl methacrylate-acrylic acid) photonic crystal is an opal structure with a periodic dielectric structure of photonic band gap, photonic crystal The photonic band gap is located at 518-527nm.

[0041] 3. Prepare the concentration of 10 with water -5 mol / L ethidium bromide (EB) ...

Embodiment 2

[0047] 1. Prepare a poly(styrene-methyl methacrylate-acrylic acid) latex particle solution with a monodisperse particle size of 0.2% and a poly(styrene-methyl methacrylate-acrylic acid) latex particle solution with a mass concentration of 0.2% in water, and sufficiently ultrasonicate to make the latex particles uniformly dispersed.

[0048] 2. With a clean carrier film as the substrate, under the constant conditions of temperature of 50°C and humidity of 50%, deposit the latex solution obtained in step 1 on the substrate by vertical deposition method, and prepare by self-assembly and arrangement on the substrate The poly(styrene-methyl methacrylate-acrylic acid) photonic crystal is obtained, and the poly(styrene-methyl methacrylate-acrylic acid) photonic crystal is an opal structure with a periodic dielectric structure of a photonic band gap.

[0049] 3. Select commercially available single-stranded DNA with amino as the end group as the probe molecule, add EB to the phosphate ...

Embodiment 3

[0054] 1. Prepare a solution of polymethyl methacrylate latex particles with a monodisperse particle size of 354nm with a mass concentration of 0.2% in water, and fully ultrasonicate to make the latex particles uniformly dispersed.

[0055] 2. With a clean carrier film as the substrate, under constant conditions of temperature 80°C and humidity 80%, deposit the latex solution obtained in step 1 on the substrate by vertical deposition method, and prepare by self-assembly arrangement on the substrate A polymethyl methacrylate photonic crystal was obtained. Polymethyl methacrylate photonic crystals are opal structures with periodic dielectric structures with photonic band gaps.

[0056] 3. Using ethanol as a solvent and concentrated hydrochloric acid as a catalyst to hydrolyze tetraethyl orthosilicate and improve it to prepare SiO 2 Sol. The specific method is as follows: 3.5ml of 28wt% tetraethyl orthosilicate and 10ml of absolute ethanol are mixed and then added with a cataly...

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PUM

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Abstract

The invention relates to a detection method of a variety of proteins and genes in a biological sample, in particular to a method which uses a photonic crystal to improve the sensitivity of biological detection. The method makes use of the characteristics of the photonic crystal to match the fluorescence labeled biological functional matter; the fluorescence labeled biological functional matter isused for detecting the specific response or specific affinity of the target biological matter; the target biological matter responds to the biological functional matter, to result in the luminous change of the biological functional matter which is labeled by fluorescence labeled molecules; a photon crystal is used for enhancing the reading and identification of fluorescence signals of the fluorescence labeled molecules; thus the method can effectively enhance the sensitivity of the detection method of the target biological matter, and further realize the high-sensitivity biological detection.

Description

technical field [0001] The invention belongs to a method for detecting multiple proteins and genes in biological samples, in particular to a method for improving biological detection sensitivity by using photonic crystals. Background technique [0002] The rapid development of life sciences has raised a large number of new topics for analytical chemistry, currently focusing on the analysis of biological macromolecules such as peptides, proteins, and nucleic acids, the analysis of biological drugs, the analysis of ultra-trace and ultra-trace biologically active substances, and even the analysis of microorganisms. Therefore, bioanalysis has become one of the most important frontier fields in the development of modern analytical chemistry. In order to meet the requirements of this situation, many analytical chemists are making continuous efforts to develop new analytical methods and techniques. Biosensor is a new type of analytical instrument developed under the background of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N21/01G01N33/50
Inventor 李明珠宋延林
Owner INST OF CHEM CHINESE ACAD OF SCI