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Nano silver-cell growth factor sustained-release composite body as well as preparation method and use thereof

A technology of growth factor and peptide growth factor, which is applied in the field of preparation of the cell polypeptide growth factor sustained-release product, can solve the problem of little effect on activity, achieve simple operation, reduce the number of medications, and alleviate the pain of patients

Inactive Publication Date: 2009-02-25
周建大
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nano-silver as a sustained-release drug carrier is rarely reported, but many studies at home and abroad have confirmed that nano-silver and sol have good biocompatibility, and when they interact with biomolecules and proteins, they have little effect on the conformation of biomolecules and proteins, that is, Generally, it has little effect on the activity of biomolecules and proteins. Studies have shown that biomolecules and proteins are mainly bound to nano-silver particles by non-specific physical adsorption (reference: Applied Spectroscopy, 58:570, 2004.)

Method used

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  • Nano silver-cell growth factor sustained-release composite body as well as preparation method and use thereof
  • Nano silver-cell growth factor sustained-release composite body as well as preparation method and use thereof
  • Nano silver-cell growth factor sustained-release composite body as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Preparation of nanosilver-human recombinant epidermal growth factor slow-release complex (Ag-rhEGF)

[0033] According to the following steps, the Ag-rhEGF complex solution can be obtained

[0034] (1) Preparation of nano-silver solution: disperse 2g of nano-silver powder with a particle size of about 30nm in pure water, and disperse it ultrasonically for 10 hours. Phosphate buffer was adjusted to pH 7.0.

[0035] (2) Add 2 g of human recombinant epidermal growth factor (rhEGF) into the above nano-silver solution, continue ultrasonic dispersion for 3 hours, shake slightly at room temperature or stand for 8 hours for reaction.

[0036] (3) Dried in a low-temperature vacuum desiccator into freeze-dried powder, and stored at -20°C.

[0037] (4) When it is configured into a solution, an appropriate amount of protective agent, preservative, stabilizer and salt solution (NaCl) can be added for mixed reaction.

[0038] Concrete formula can be as follows:

[0039] AG+hEG...

Embodiment 2

[0048] Preparation of nanosilver-basic fibroblast growth factor slow-release complex (Ag-bFGF)

[0049] According to the following steps, the Ag-bFGF complex solution can be obtained

[0050] (1) Preparation of nano-silver solution: disperse 2g of nano-silver powder with a particle size of about 30nm in pure water, and disperse it ultrasonically for 10 hours. Phosphate buffer was adjusted to pH 7.0.

[0051] (2) Add 2 g of human fibroblast growth factor (bFGF) into the nano-silver solution. Continue to ultrasonically disperse for 3 hours, shake slightly at room temperature or stand still for 8 hours.

[0052] (3) Dried in a low-temperature vacuum desiccator into freeze-dried powder, and stored at -20°C.

[0053] (4) When it is configured into a solution, an appropriate amount of protective agent, preservative, stabilizer and salt solution (NaCl) can be added for mixed reaction.

[0054] Concrete formula can be as follows:

[0055] AG+bFGF complex 4mg

[0056] Sodium Benz...

Embodiment 3

[0064] Preparation of nano-silver-human fibroblast growth factor sustained-release complex containing nano-silica sol

[0065] (1) Preparation of nano-silver solution: disperse 2g of nano-silver powder with a particle size of about 30nm in pure water, and disperse it ultrasonically for 10 hours. Phosphate buffer was adjusted to pH 7.0.

[0066] (2) Add 2 g of human fibroblast growth factor (bFGF) into the above nano-silver solution, continue ultrasonic dispersion for 3 hours, shake slightly at room temperature or stand for 8 hours for reaction.

[0067] (3) Add 10 mg of nano-silica sol to the solution prepared in (2), and disperse by ultrasonic for 4 hours.

[0068] (4) Dried in a low-temperature vacuum desiccator into freeze-dried powder, and stored at -20°C.

[0069] (5) When it is configured into a solution, an appropriate amount of protective agent, preservative, stabilizer and salt solution (NaCl) can be added for mixed reaction.

[0070] Concrete formula can be as fol...

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Abstract

The invention relates to a sustained-release matter of a cell peptide growth factor. The invention further relates to a preparation method of the sustained-release matter of the cell peptide growth factor and a use thereof. The provided sustained-release matter of the cell peptide growth factor is a nano-silver-cell growth factor complex, the peptide growth factor bonds with Ag by the nano-absorption or / and -COO, -CN and -H, thereby being absorbed on the surface of the nano-silver to form the complex. The sustained-release matter utilizes the natural anti-inflection effect of the nano-silver to provide a clean micro-environment for the biological action of the growth factor, promotes faster repair and healing of wound / wound surface, reduces adverse reactions, reduces the times of drug administration and the waste of drugs, alleviates suffering of patients and simultaneously reduces medical costs.

Description

technical field [0001] The invention relates to a sustained release product of cell polypeptide growth factor. The invention also relates to the preparation method and application of the cell polypeptide growth factor sustained-release substance. Background technique [0002] Cell growth factors are a class of protein and polypeptide substances that regulate cell proliferation and differentiation. Their molecular weights range from several hundred to tens of thousands, and they are often called polypeptide growth factors. Compared with classic polypeptide and protein hormones, polypeptide growth factors do not have specific endocrine glands and endocrine cells, but are released and diffused to target cells through the paracrine and autocrine of some cells, thereby coordinating the unity of the body itself and the response to the outside world. Reaction. Due to the important theoretical and practical significance of polypeptide growth factors in the field of life sciences a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K38/18A61K33/38A61K9/16A61K9/00A61P17/02A61P27/02A61P15/00A61P1/02A61P3/10A61K47/52
Inventor 周建大王少华
Owner 周建大
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