Preparation of carbamide detection test paper for dairy food and carbamide detection method

Abstract

The invention provides a preparation method of urea detection test paper in dairy and a detection method of urea; urease source powder is taken and mixed with pH indicator with the pKHIn more than 6.8; the mixture is diluted by deioned water and the filter solution or the upper suspension liquid after natural deposition is taken, uniformly fixed on a carrier with good water absorption by the forms of coating, printing or soaking, and dried subsequently, thus forming the urea detection test paper; the detection method of the dairy comprises the steps as follows: firstly, common pH test paper is used for detecting the dairy to be detected; whether the dairy to be detected is in normal pH range 6.3-6.8 or not is checked; if so, the urea detection is directly carried out; if not, the pH valueis adjusted to the normal pH range and the urea detection is carried out subsequently; one piece of urea detection test paper dips the dairy to be detected and the color change of the test paper is observed; if the color of the test paper is not changed, the dairy is qualified; if the color of the test paper changes, the dairy is mixed with the urea, thus carrying out qualitative judgment on the dairy; and the displayed color is compared with a standard colorimetric card, so as to carry out semi-quantitative detection of the urea in the dairy.

Description

Preparation of urea detection test paper in dairy products and urea detection method technical field The invention relates to rapid qualitative or semi-quantitative detection of urea in food safety monitoring, in particular to a qualitative or semi-quantitative determination method for urea in urea-adulterated milk and a preparation method for the urea detection test paper used therein. Background technique Urea detection is a common indicator of laboratory testing, used for food industry, feed industry safety supervision monitoring and anti-counterfeiting and environmental protection testing, such as detection of urea-adulterated fresh milk, adulteration of animal protein materials, urea content in swimming pool water detection and so on. At present, there are many laboratory methods for urea detection. Most of the rapid determination of urea in milk uses the colorimetric method. The chromogenic reagents used mainly include p-dimethylaminobenzaldehyde, nitrite-Gries reag...

AI Technical Summary

Problems solved by technology

It can be seen that there is no simple, fast and portable method suitable for urea detection at present

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