Alkali-fast high-activity alkalescent xylanase and encoding gene thereof

Abstract

The invention belongs to the genetic engineering technology field, and specifically relates to a new alkaline xylanase and a code gene thereof. The invention discloses a sequence of the gene and a coding area thereof. Escherichia coli DH5alpha / Mn22 / 6p / Kxyn which contains the gene plasmid is collected in China Center for Type Culture Collection (CCTCC) with the collection No. of M208201. The xylanase Kxyn is proved to have strong alkali resistance and thermal stability through the biological verification, thereby being capable of specially degrading xylan. The xylanase Kxyn can be used for paper making and other related fields.

Description

technical field The invention relates to the field of genetic engineering, in particular to an alkali-resistant and highly active alkaline xylanase and the cloning of its gene. technical background Xylan is a hybrid five-carbon sugar. Most of the main chain is D-type xylopyranose connected by β-1,4 glycosidic bonds. The main side chain substituents are arabinose and glucose. Aldonic acid, 4-0-methylglucuronic acid, acetyl, galactosyl, etc. Xylan is the main component of hemicellulose, accounting for 1 / 3 of plant carbohydrates, and is the second largest renewable resource after cellulose. Therefore, hydrolysis of xylan is an important step in the utilization of hemicellulose. Xylanase is widely used as an additive in the feed industry, an improver in the food industry, and a biological bleaching agent for pulp in the paper industry (Polizeli et al., 2005). Xylanase was first used in the feed industry. Xylanase can destroy plant cell walls, release nutrients and improve n...

AI Technical Summary

Problems solved by technology

However, due to problems such as low yield of xylanase or insufficient tolerance of the enzyme, the large-scale use of the enzyme in industry is limited.

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