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Peach family plant seed identification method

A technology for plants and peaches, which is applied in the field of identifying species of peaches, and can solve problems such as the classification of undetermined base sequences

Active Publication Date: 2013-07-17
TSUMURA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the nucleotide sequences of many species of the genus Peach have not been determined and classified, so there is no example of judging the species based on this.

Method used

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  • Peach family plant seed identification method
  • Peach family plant seed identification method
  • Peach family plant seed identification method

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Embodiment 1

Heilongjiang Province Duerbert Mongolian

Zhixian Jiaodu Xibo Township Wushu City 44092 Northeast apricot (Prunus mandshurica) Luoyueping, Helong City, Jilin Province 70706

Northeast apricot (Prunus mandshurica)

Attached to Tumen River Estuary, Hunchun City, Jilin Province

close 71987 Northeast apricot (Prunus mandshurica) Wuchang City, Heilongjiang Province

[0054] DNeasy (registered trademark) Plant Mini Kit (Qiagen) was used to extract DNA from about 200 mg of the sample, and each region was amplified by PCR using thermal cycler PE-2000 (Applied Bio Systems). The composition of the PCR reaction solution is as follows: 10×Ex-Buffer (TaKaRa) 5 μl, dNTP mixture (TaKaRa) 5 μl, forward primer (5′-GTT TCT TCT CATCCA GCT CC-3′) (10 pmol / μl) 1.0 μl, reverse Primer (5'-GAA AGA GTC AATATT CGC CC-3') (10 pmol / μl) 1.0 μl, template DNA 1.25 μl, Ex-Taq (TaKaRa) 0.25 μl, DMSO 5 μl, D.D.W. 31.5 μl (TaKaRa). Reaction cycle, (94°C, 1 minute; 48°C, 2 m...

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Abstract

The present invention relates to a method for determining the species of the peach genus vegetables in high accuracy. In particular, the invention relates to a method for discriminating the species of the peach genus contained in the sample to be detected. The method includes comparing the base sequence of the introne portion region of a ribosome gene rp116 in the chloroplast genome contained in the vegetable of the sample to be detected, with at least a base sequence selecte4d from the sequence numbers.

Description

[technical field] [0001] The present invention relates to a method for identifying species of plants of the genus Plulus by means of genetic engineering. [Background technique] [0002] Almond (キヨウニン) is a crude drug recorded in the first supplement of the 14th bureau of the Japanese Pharmacopoeia, with apricot (Prunus armeniaca Linne) and wild apricot (Prunus armeniaca Linnevar.ansu Maximowicz) as its base plants. But in China, besides this, there are crude drugs containing Prunus sibirica Linne or Prunus mandshurica in circulation. On the other hand, peach kernel (トウニン) is a crude drug based on peach (Prunus persica Batsch) and mountain peach (Prunus persica Batsch var. However, in China, besides this, there is a crude drug of Prunus mira in circulation. However, it is difficult to discriminate whether it is a true crude drug containing only the original plant or a crude drug also containing plants other than the original plant. Therefore, there are quality and safety p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 山路弘树
Owner TSUMURA