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Method for detecting soil microbial biomass nitrogen

A technology of soil microorganisms and biomass, which is applied in the direction of biological testing, chemical analysis by titration, material inspection products, etc., can solve the problems of exacerbating the difficulty of transferring products, the inadvisability of chromium and potassium sulfate, and the existence of losses. The effect of continuous development, good accuracy and easy cleaning

Inactive Publication Date: 2013-01-30
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Using this method to measure soil microbial biomass nitrogen has the following disadvantages: ① It is not advisable to use chromium potassium sulfate as a reducing agent in this method
②The method itself takes a long time
After the digestion is finished and cooled, the solid product in the digestion tube needs to be scraped out bit by bit, so there will be losses during the transfer to the retort
In addition, in order to speed up the process of the test, the added zeolite will freeze in the middle of the bottom of the test tube, which intensifies the difficulty of transferring the product

Method used

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  • Method for detecting soil microbial biomass nitrogen
  • Method for detecting soil microbial biomass nitrogen
  • Method for detecting soil microbial biomass nitrogen

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The nitrogen content in the standard solution of acetanilide was determined by comparing the original method and the improved method.

[0033] With reference to Document 4, the nitrogen content in the imported acetanilide standard solution was determined using the improved method of the present invention. Add 5.0ml of extract solution once in the digestion tube, mix accelerator 3g (accelerator mass composition is K 2 SO 4 : CuSO 4 : Se=90:9:1), and 8ml of concentrated sulfuric acid, shake well; the solution is heated to 340°C by means of gradient temperature rise, constant temperature, after 3h, cool; the solution in the digestion tube is poured into the distillation flask , Wash the digestion tube 2-3 times with distilled water, add NaOH solution for distillation at the same time, receive boric acid, and titrate with standard hydrochloric acid solution.

[0034] The specific process is as follows:

[0035] 1) Preparation of acetanilide standard solution: Take 1.929...

Embodiment 2

[0062] Determination of microbial biomass nitrogen in soil using a modified method.

[0063] The specific implementation process:

[0064] The test steps are:

[0065] 1) Fumigation-extraction: Take 30g of fresh soil (equivalent to about 25g of dry soil) in a small beaker, put it into a vacuum desiccator with 30ml of chloroform (with zeolite), 30ml of NaOH and wet filter paper, and airtightly pump to Chloroform boiling 3min, close the valve. Cultivate in the dark at 25°C for 24 hours, open the desiccator to check for air leakage, if there is no air leakage, take out the chloroform and NaOH, and repeatedly pump air with a vacuum pump until the soil does not smell the smell of chloroform. In addition, no fumigation was used as a control. Shake the fumigated and non-fumigated soil samples with 100ml potassium sulfate solution for 30min, and filter;

[0066] 2) Determination: Add 5.0ml of extract solution successively in the digestion tube, mix accelerator (mass ratio is K 2 ...

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Abstract

The invention relates to a method for measuring biomass nitrogen of soil microorganisms, which uses a fumigation-leaching method for processing soil samples and comprises the following steps: using a non-fumigated soil sample as a contrast; oscillating and leaching the fumigated and the non-fumigated soil samples with 0.5 M potassium sulfate solution of which the weight volume is 4 times that of soil samples; filtering the mixture to obtain a leaching liquor; adding 5 milliliters of the leaching liquor, 3 grams of mixed accelerator, and 8 milliliters of concentrated sulfuric acid into a digestion tube; shaking the mixture evenly; heating the mixture slowly until the sulfuric acid is fuming; digesting the mixture at a high temperature for 3 hours until the solution is clarified; cooling the solution; transferring the digestion products into a distillation flask without loss; adding a NaOH solution into the digestion products to perform distillation at the same time; receiving the product with a boric acid; and titrating the product with a standard hydrochloric acid solution. The method has the advantages of good repeatability, high reproducibility, simplicity and practicability.

Description

technical field [0001] The invention relates to the determination of microbial biomass nitrogen in soil, in particular to an improved determination method of soil microbial biomass nitrogen. Background technique [0002] Soil microbial biomass refers to the volume of soil less than 5μm 3 -10μm 3 The total amount of living microorganisms, whose nutrients account for a small proportion of the corresponding elements in the soil, but it is of great significance in evaluating its effect on crop nutrition. Because it is very active and can quickly participate in the nutrient cycle, it is not only the driving force of the nutrient cycle process and the "converter" of organic matter entering the soil, but also the internal supply mechanism of soil energy and nutrients, especially C, N, P, S and other elements. "Source" and "Library" (Reference 1: Jenkinson DS, Ladd J N, Microbial biomass in soil: Measurement and turnover, Soil Biochemistry, 1981, (5): 415-471). [0003] Microbial...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50G01N31/16
Inventor 周桦宇万太张璐马强
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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