Sargassum thunbergii seedling quick-propagation method using leader branch segment tillering method

A technology of sargassum and sargassum algae, which is applied in botany equipment and methods, seaweed cultivation, plant cells, etc., can solve the problems of high cost and long cultivation period, and achieve low cost, large seedling production, and seedling cultivation Inexpensive effect

Inactive Publication Date: 2009-05-06
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method also has its own disadvantages. The main reason is that its cultivation period is relatively long. It takes at least 6-8 months from the cultivation of young leaves to the length that can clamp seedlings.
In addition, the leaf tissue culture method also requires a higher cost

Method used

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  • Sargassum thunbergii seedling quick-propagation method using leader branch segment tillering method
  • Sargassum thunbergii seedling quick-propagation method using leader branch segment tillering method
  • Sargassum thunbergii seedling quick-propagation method using leader branch segment tillering method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The dioecious strains of Sargassum under natural conditions are difficult to distinguish from the morphological characteristics alone. However, sargassum can be divided into two different types in terms of external morphology. One type of strain has sparse leaves and a slender main stem ( figure 1 A); Another type of algae strain has thick leaves and strong main stem ( figure 1 B), statistics show that the biomass of two different types of main branches of the same length is 1.3 to 2.1 times that of the former.

[0027] The present invention selects main branches of two different types of algal strains to carry out culture experiments respectively. After seaweed samples are collected, use brushes and tweezers to remove debris and attached organisms on the surface. Select the longer main branch, and use KMnO with a mass concentration of 1‰ 4 Treat for 1.5 to 2 minutes, and then rinse with a large amount of sterile sea water for 3 to 4 times. If temporary cultivation...

Embodiment 2

[0040]The experiment was conducted from August 7, 2005 to November 28, 2005. The samples were collected from the intertidal zone of Qingdao Second Beach (35.35°N, 119.30°E, 10-35cm below the water surface).

[0041] The algae was collected from the intertidal zone of Qingdao No. 2 Bathing Beach on August 2, 2005. Different types of main branches ( figure 2 A), cultured after cutting the segments respectively, see the development process of different types of main branch segments within two weeks figure 2 B and figure 2 c.

[0042] The culture conditions are: temperature 17℃; light intensity 25μmol / m 2 per s; photoperiod 12:12h (light: dark);

[0043] The medium used is: ES medium + BG-11 seawater nutrient solution, that is, the ingredients of BG-11 formula are added to the general ES seawater culture medium;

[0044] BG-11 Formula: H 3 BO 3 2.86μg / l, MnCl.4H2O 1.81μg / l, ZnSO 4· 7H2O0.222μg / l, NaMoO 4· 2H2O 0.39 μg / l, CuSO 4· 5H2O 0.079μg / l, Co(NO3) 2· 6H2O 0.04...

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Abstract

The invention relates to the propagation of Sargassum thunbergii, in particular to a method for quickly propagating the Sargassum thunbergii by a method of main branch fragment tillering, which comprises the following steps: 1) alcohol with a volume concentration of 75 percent used by the main branch of the Sargassum thunbergii is adopted for dipping the main branch for 25 to 23 seconds; and then a plurality of disinfected seawater is adopted for quickly washing the main branch for 3 to 4 times; 2) the main branch of the Sargassum thunbergii strain is sheared into fragments with a length of 2 to 3cm; the fragments are arranged in a container to culture; universal ES seawater culture liquid is adopted as a medium for ventilation culturing; and when the tillers of the fragments can achieve 1.5 to 3.5cm, the fragments are taken out from the container and the tillers are cut off by a disinfected bistoury; and 3) the cut off tillers are continuously propagated and cultured by adopting the conditions in the step 2) or directly used for the large-scale gripping mariculture. The method is simple to be operated and low in cost; regenerated seedlings can be produced in a circularly; moreover, the method plays the remarkable role of protection on the natural environment.

Description

technical field [0001] The invention relates to the breeding of sargassum, in particular to a method for rapidly propagating sargassum seedlings by the main branch segment tillering method. Background technique [0002] Sargassum algae are dark brown, shaped like a rat tail, 3 to 50 cm high, up to 120 cm high. Grows on mid-tidal rocks or bogs. Visible throughout the year, the peak growth period is from March to July. Widely distributed in my country's coastal areas. Sage algae is rich in nutrients and low in alginate, so it is a high-quality bait for important seafood such as sea cucumbers. Sargassum was seldom used by people before, but with the rapid expansion of the scale of sea cucumber and other seafood farming, the natural growth of Sargassum has been collected in large quantities. At present, the resources of Sargassum in many breeding areas are almost exhausted. [0003] The lack of sargassum resources and the poor quality of feed have largely restricted the heal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00C12N5/04
CPCY02A40/80
Inventor 叶乃好毛玉泽方建光邹健于守团
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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