Cyclopenthiazide induced novel epilepsy model

Abstract

The invention discloses an application of cyclopenthiazide in the preparation of an epilepsy animal model as a drug for inducing epilepsy, the model adopts the cyclopenthiazide for carrying out the local injection of brain of a mammal, the injection is carried out twice or more in 2d-20d with 0.01-0.5 mumol / time, and the total safe effective dose is 0.1-5mumol. A rat appears the II-V-class epilepsy attack of the Racine classification method after the injection, and an EEG simultaneously records the electroencephalogram discharge with the appearance of the epilepsy. The application takes the cyclopenthiazide as an inducer for establishing the new epilepsy animal model, thereby providing a beneficial tool for researching the pathogenesis of the epilepsy, seeking a target point for inhibiting the epilepsy attack and screening the drugs with higher effectiveness for the drug-resistant refractory epilepsy.

Description

technical field [0001] The present invention relates to the application of Cyclothiazide (CTZ) as an epileptogenic agent, in particular to the establishment of a novel animal model of epilepsy by inducing epileptic seizures in rats by intracerebral local injection. Background technique [0002] Epilepsy is one of the most common neurological diseases, with an annual incidence of about 35 / 100,000, and a prevalence of about 0.5%. There are 30 million people with epilepsy worldwide. There are 450,000 new epilepsy patients in my country every year, and the total number of patients is about 6.5 million. About one quarter of the patients cannot effectively control their seizures after drug treatment, which is called intractable epilepsy (intractable epilepsy), and about 70% of intractable epilepsy is temporal lobe epilepsy (temporal lobe epilepsy, TLE). The most prominent clinical feature of temporal lobe epilepsy is long-term recurring spontaneous epileptic seizures. Temporal ...

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Problems solved by technology

Qi Jinshun et al. found that cyclopentylazine can induce epileptiform activity in cultured neurons and neurons in the CA1 region of the hippocampus of anesthetized animals. , et al.J Physiol, 2006, 605-618); but the results obtained in the in vitro experiment cannot be directly used to speculate or explain its function or role in the intact body (Physiology, edited by Yao Tai, People's Health Publishing House, 2000, Beijing); and some scholars believe that giving cyclopentylthiazine alone cannot induce epileptic seizures in animals (Francesco Fornai, Carla L.Busceti, Alexei Kondratyev et al.European Journal of Neuroscience, 2005, 21: 455—463)

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