Chloride ion passage gene fragment related to pig intramuscularly fat deposition and use thereof
A technology of intramuscular fat and fragments, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve problems such as difficulty in obtaining breakthroughs, failure to achieve expected goals, and reduced lean meat rate, and achieve huge economic value and social significance. The effect of improving the accuracy of selection and shortening the time of selection
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Embodiment 1
[0018] Example 1. Discovery of DNA fragments related to pig intramuscular fat deposition
[0019] Generally speaking, the intramuscular fat deposition of Rongchang pigs is about 4.0%-5.0%, which is about 2 times that of lean pigs. In Rongchang, Chongqing, the feeding test and slaughter test of the effect of the ratio of dietary lysine / digestible energy on the intramuscular fat deposition of Rongchang pigs found that some individual Rongchang pigs had super-deposited intramuscular fat, and the amount of intramuscular fat deposition could reach 8.8%-9.1 %, almost double that of ordinary Rongchang pigs.
[0020] Using pig Affymetrix (Affymetrix, Inc., USA) oligonucleotide chips, the gene transcription and expression profiles of the longissimus dorsi tissue of Rongchang pigs with super-deposited intramuscular fat were scanned, and several DNA fragments were obtained as candidate fragments.
[0021] 1. Amplification of DNA fragments
[0022] Design a primer pair based on one of t...
Embodiment 2
[0041] Embodiment 2, the application of the dna fragment relevant to pig intramuscular fat deposition
[0042] Using the cDNA of the muscle tissue of 36 90kg Rongchang pigs as a template, the following primers were used for PCR amplification:
[0043] Upstream primer (sequence 3 of the sequence listing): 5'-CAGAAAGAACATCCAGGACC-3';
[0044] Downstream primer (sequence 4 of the sequence listing): 5'-CTTAGCACAGGCATGACGAT-3'.
[0045] Then the amount of intramuscular fat deposition in the longissimus dorsi muscle tissue of the 36 Rongchang pigs was detected, and the results are shown in Table 1.
[0046] Table 190kg different genotype Rongchang pig intramuscular fat deposition (%)
[0047]
[0048] The same letter indicates no significant difference between genotypes, and different letters indicate significant difference between genotypes.
[0049] In the 90kg Rongchang pigs, the pigs with AA genotype had the highest amount of intramuscular fat deposition, followed by the pig...
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