Method for producing crystal D-ribose

A technology of crystallization and ribose, applied in the field of microbial fermentation to produce D-ribose, which can solve the problems of unresolved product quality, low yield, environmental pollution, etc.

Active Publication Date: 2009-07-08
SHANGHAI ACEBRIGHT PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has a very low yield and is not suitable for large-scale industrial production
[0004] In the 1960s, electrolysis of ribonucleic acid appeared to produce D-ribose, but due to the use of mercury electrodes, it caused serious environmental pollution
In addition, although there are many patents on th

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of seed solution:

[0038] 1) Take the frozen tube strain of Bacillus subtilis (Bacillus subtilis), and inoculate it on the blank slope of the primary seed (medium ratio: sorbitol 5.0g / L, peptone 10.0g / L, yeast extract 20.0g / L, K 2 HPO 4 2.0g / L, KH 2 PO 4 1.0g / L, NaCl 2.0g / L, agar 20.0g / L, pH natural), cultured at 37.0°C for 20-26hr. Get the first-class slant seeds and inoculate them on the production blank slant (medium ratio: glucose 0.5g / L, peptone 10.0g / L, yeast extract 20.0g / L, K 2 HPO 4 2.0g / L, KH 2 PO 4 1.0g / L, NaCl 2.0g / L, agar 20.0g / L, PH natural), cultivated at 37.0°C for 20-26hr, and prepared slant seeds for production;

[0039] 2) Take the above-mentioned production slant seeds and inoculate them in 200 / 500mL liquid medium (glucose 20.0g / L, yeast extract 2.0g / L, K 2 HPO 4 3.0g / L,KH 2 PO 4 1.0g / L, PH natural) in the seed bottle, cultivated at 37.0 ℃ for 14hr, and prepared the shake flask seed for subsequent use.

[0040] 3) Take t...

Embodiment 2

[0043] Mixed sugar source fermentation:

[0044] Insert 2.0mL of shaking flask seeds into a 250mL Erlenmeyer flask containing 20mL of sterilized culture medium, and the fermentation medium components are: glucose 120.0g / L, sucrose 100.0g / L, corn steep liquor 26.0g / L, (NH 4 ) 2 SO 4 9.0g / L, K 2 HPO 4 2.0g / L, KH 2 PO 41.0g / L, yeast powder 1.0g / L, MgSO 4 0.5g / L, MnSO 4 0.5g / L, calcium carbonate 20.0g / L, pH 7.1. Cultivate on a rotary shaker at 37.0°C and 260r / min for 72hrs. After the fermentation was completed, the D-ribose content was detected by the orcinol method to be 103.1 g / L, and the weight conversion rate of total sugar was 46.86%.

Embodiment 3

[0046] Mixed sugar source fermentation:

[0047] In a 7L fermenter equipped with 3.5L medium, insert 200ml of shake flask seeds to start fermentation. The components of the fermentation medium are: glucose 100.0g / L, sucrose 100.0g / L, corn steep liquor 26.0g / L, (NH 4 ) 2 SO 4 9.0g / L, K 2 HPO 4 2.0g / L, KH 2 PO 4 1.0g / L, yeast powder 1.0g / L, MgSO 4 0.5g / L, MnSO 4 0.5g / L, calcium carbonate 20.0g / L, pH 7.0-7.2, glucose and sucrose single disinfection, sterilized at 121.0°C for 20min, other materials at 121.0°C for 30min. Cultivate at 37.0°C, ventilation rate 0.5vvm, tank pressure 0.04Mpa, stirring speed 500r / min for 58hrs, the D-ribose content is 91.6g / L detected by the orcinol method after fermentation, and the total sugar weight conversion rate is 45.8%.

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PUM

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Abstract

The present invention provides a process for producing crystal D-ribose, including the following steps: (1) accessing air to perform aerobic fermentation to Bacillus subtilis in the fermentation medium containing a substrate composed of starch, starch hydrolysis sugar, monosaccharide and disaccharide mixing sugar, and then collecting the D-ribose supernatant from the fermentation products; (2) adopting cationic resin and anion resin desalinization processing to the D-ribose supernatant collected by the step (1), and then concentrating the desalinized solution to obtain a sugar; (3) adopting achemical method or chromatography method to separate and refine the obtained concentrated sugar to get a crystal D-ribose. The crystal D-ribose obtained by the method of the invention has a white content of more than 90% and HPLC purity of more than 99.5%, can be directly used as food additives, and can also be easily used for preparation of anti-virus and anti-cancer drugs.

Description

technical field [0001] The invention relates to a method for producing D-ribose by fermentation, in particular to a method for producing D-ribose by a microbial fermentation method. Background technique [0002] D-ribose is a component of the genetic material ribonucleic acid (RNA) and several coenzymes and vitamins in organisms. It is a very important substance in physiology and is currently widely used in food, medicine, cosmetics and feed industries. . In the pharmaceutical industry, D-ribose has always been an important raw material for the synthesis of vitamin B2, and it is also an important raw material for the synthesis of antiviral and antitumor nucleoside drugs, and its role has become increasingly prominent. In addition, in recent years, it has also been found that D-ribose can increase the ATP level in the body, and has the functions of anti-fatigue and hypoxia resistance. [0003] The production and preparation of D-ribose is initially isolated from natural sub...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12N1/20C12R1/125
Inventor 李金亮唐如星
Owner SHANGHAI ACEBRIGHT PHARMA CO LTD
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