Discovery method for protein post-translational modification

Abstract

The invention provides a detecting method of post-translational protein modification. The method comprises the following steps: calculating spectrogram difference vectors among all spectrograms by means of peptide chromatogram retaining time and peptide quality in experimental tandem mass spectrum data of protein samples; establishing candidate modification mass intervals probably containing modification mass; estimating mixed distribution of the spectrogram difference vectors on each candidate modification mass interval, and calculating the standard deviation of each distribution inside the mixed distribution so as to determine distributions inside the candidate modification mass intervals caused by post-translational protein modification according to the standard deviation; calculating the average value of the distributions caused by post-translational protein modification; obtaining an accurate mass experimental value of the post-translational protein modification according to the mass component of the average value; and obtaining the influence of post-translational protein modification on peptide chromatogram retaining time according to the retaining time component of the average value. The detecting method has the advantages of high efficiency, accuracy and robustness.

Description

technical field [0001] The invention relates to protein post-translational modification in biogenetics, in particular to a method for discovering protein post-translational modification. Background technique [0002] It is well known that the genetic information of most organisms is stored in DNA. DNA generates messenger RNA through the process of transcription, and messenger RNA generates protein through the process of translation, thus realizing the transmission of genetic information from DNA to RNA and then to protein. This process is also known as the central dogma of life. In the process of protein translation from RNA, the chain molecules formed by connecting 20 kinds of amino acids in sequence with peptide bonds are called peptides, and the peptides whose molecular weight reaches a certain level are called proteins. After most proteins are translated, some functional groups (such as adding acetyl to the N-terminal of the protein) will be added to some amino acids in...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the defects of high computational complexity and accuracy affected by the quality of experimental tandem spectra in the process of detecting protein post-translational modification using fragment ion spectrum information, thereby providing an efficient and accurate protein translation post-modification discovery method

Images