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38 results about "Peptide mass" patented technology
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Isotope-coded ionization-enhancing reagents (ICIER) for high-throughput protein identification and quantitation using matrix-assisted laser desorption ionization mass spectrometry
InactiveUS6905879B2Quantitative precisionReduce disulfide bondSamplingComponent separationLaser desorption ionization mass spectrometryArginine
Arginine-containing cysteine-modifying compounds useful for MALDI-MS analysis of proteins are provided. These compounds termed isotope-coded ionization enhancement reagents (ICIER) can provide ionization enhancement in MALDI-MS, relative quantitation, and additional database searching constraints at the same time without any extra sample manipulation. More specifically, ICIER increase the ionization efficiency of cysteine-containing peptides by attachment of a guanidino functional group. ICIER also increase the overall hydrophilicity of these peptides due to the hydrophilic nature of ICIER and thus increase the percentage of recovery of these peptides during sample handling and processing such as in-gel digestion or liquid chromatography. Finally, a combination of both light and heavy ICIER provides an accurate way to obtain relative quantitation of proteins by MALDI-MS and additional database searching constraints (number of cysteine residues in every single peptide peak) to increase the confidence of protein identification by peptide mass mapping.
Owner:GENETICS INST INC
Method For Simultaneous Calibration of Mass Spectra and Identification of Peptides in Proteomic Analysis
InactiveUS20080203284A1Calibration apparatusIsotope separationMass Spectrometry-Mass SpectrometryProteomic Profile
The invention relates to a mass spectrometry calibration system that may be performed in real-time using the information contained within a sample without the addition of specific calibrants. When applied to a sample, such as a proteomic sample, the calibration system may identify the exact masses of peptides in the sample. The system involves the use of mathematical algorithms that iteratively estimate the error in the measurement and update the calibration parameters accordingly; thereby resulting in peptide mass identification.
Owner:CEDARS SINAI MEDICAL CENT
Methods for Acquisition and Deductive Analysis of Mixed Fragment Peptide Mass Spectra
A method for acquiring and analyzing polypeptide mass spectra comprising: creating first and second sets of fragmented ions using first and second fragmentation techniques; obtaining mass spectra of the first and second sets of fragmented ions; searching a database of fragments of a first fragment pair type and of a second fragment pair type to respectively provide a set of M ranked and a set of N ranked best matching polypeptide sequences; subtracting synthetic spectra corresponding to each of the M and N ranked sequences from the mass spectra to provide first and second sets of modified mass spectra; searching the database of fragments of the second fragment pair type and of the first fragment pair type to provide third and fourth sets of polypeptide sequences providing best matches to the first and second sets of modified spectra, respectively; and creating a cumulative ranking of the third and fourth sets.
Owner:THERMO FINNIGAN
Method of screening action target of cardiac glycoside medicine using protein composition technology
InactiveCN1793933APreparing sample for investigationDispersed particle separationProtein compositionBiology
A method for selecting action target of cardiac glucocide medicine by utilizing protein multistudy technique setting up model of cadiocytes culture and action on cadiocytes by cardiac glucocide, preparing protein sample, carrying two ¿C way gel electrophoresis and collecting image for analysis to obtain difference expression protein, using mass spectrum to determine difference protein peptide mass finger atlas to obtain DNA series for selecting out difference protein, verifying biological property of difference expression protein and using protein multistudy technique to select target of cardiac glucocide medicine.
Owner:SHANDONG UNIV
Peptide mass spectrometry rich in daughter ions
InactiveUS20060022129A1Optimize allocationDistribute quicklyParticle separator tubesIsotope separationMass Spectrometry-Mass SpectrometryMass spectrometry imaging
Methods of analysing peptides by mass spectrometry are disclosed. In particular, methods of analysing peptides by fragmentation mass spectrometry (MSn, where n is at least 2) are disclosed. The methods involve derivatisation of a peptide at its N-terminus such that peaks corresponding to both a and b (and y) daughter ions are identifiable in fragmentation mass spectra of the derivatised peptide. The fragmentation mass spectra of the derivatised peptide contain additional information (relative to the fragmentation mass spectra of the underivatised peptide) useful for determination of the amino acid sequence of the peptide.
Owner:OXFORD GENE TECH IP
In-gel tagging and in-gel digestion for phosphoproteins analysis and phosphorylation site identification
The present invention relates to a method for phosphorylation site-specific labeling of phosphoproteome with a site-specific tagging reagent and analyzing of the resulting labeled one, more especially, a method for in-situ tagging of phosphorylation sites of phosphoproteins retained in polymeric gel with a nucleophilic tagging reagent. It also relates a method for generating new proteolytic cleavable sites at formerly phosphorylation sites by a proper choice of a nucleophilic tagging reagent. It also relates to a method for phosphopeptides analysis and phosphorylation site identification by in-gel digestion of the previously in-gel tagged proteins and subsequent mass analysis of the resulting peptides. The invention provides in-gel chemical tagging method for phosphoaminoacid residue of phosphoproteins retained in polymeric gel matrix. Phosphoprotein can be immobilized into gel matrix by a variety of methods such as gel electrophoresis. The immobilized phosphoproteins are retained in gel matrix during tagging reaction to phosphorylated aminoacid residue of phosphoproteins, and the resulting tagged proteins are also retained in gel matrix till following purification steps like washing of the tagging reagents are accomplished. The tagged proteins is digested by protease, and the resulting digested peptides is released from gel into solution and applied for peptide mass analysis.
Owner:KOREA BASIC SCI INST
Method for simultaneous calibration of mass spectra and identification of peptides in proteomic analysis
Owner:CEDARS SINAI MEDICAL CENT
Crosslinking dipeptide rapid identification method
ActiveCN106033501AFast searchImprove identification efficiencySpecial data processing applicationsDipeptidePeptide sequence
The present invention provides a method for rapid identification of cross-linked dipeptides, comprising: 1) extracting effective spectral peaks in the tandem spectrogram to be identified, and searching for the fragment index to obtain the corresponding peptide sequence as a candidate α according to the mass corresponding to each effective spectral peak Peptide sequence, wherein the fragment index records the mass of each fragment and its corresponding peptide sequence; 2) For each candidate α-peptide sequence, calculate the corresponding β-peptide mass according to the precursor ion mass of the tandem spectrum to be identified, Then obtain the corresponding candidate β-peptide sequence, combine the candidate α-peptide sequence and the corresponding candidate β-peptide sequence to obtain a candidate cross-linked dipeptide; 3) finely match the candidate cross-linked dipeptide obtained in step 2) with the tandem spectrum, Get the identification result. The invention does not need to use a special cross-linking agent; the search speed is fast, the identification efficiency is high; and the search sensitivity is high.
Owner:INST OF COMPUTING TECH CHINESE ACAD OF SCI +1
Stable isotope, site-specific mass tagging for protein identification
InactiveUS20050124014A1Improve accuracyImprove efficiencyBacteriaSamples introduction/extractionPresent methodProtein-protein complex
Proteolytic peptide mass mapping as measured by mass spectrometry provides an important method for the identification of proteins, which are usually identified by matching the measured and calculated m / z values of the proteolytic peptides. A unique identification is, however, heavily dependent upon the mass accuracy and sequence coverage of the fragment ions generated by peptide ionization. The present invention describes a method for increasing the specificity, accuracy and efficiency of the assignments of particular proteolytic peptides and consequent protein identification, by the incorporation of selected amino acid residue(s) enriched with stable isotope(s) into the protein sequence without the need for ultrahigh instrumental accuracy. Selected amino acid(s) are labeled with 13C / 15N / 2H and incorporated into proteins in a sequence-specific manner during cell culturing. Each of these labeled amino acids carries a defined mass change encoded in its monoisotopic distribution pattern. Through their characteristic patterns, the peptides with mass tag(s) can then be readily distinguished from other peptides in mass spectra. The present method of identifying unique proteins can also be extended to protein complexes and will significantly increase data search specificity, efficiency and accuracy for protein identifications.
Owner:LOS ALAMOS NATIONAL SECURITY
Anti-dry soft and moist cream and preparation method thereof
InactiveCN104398461AModerate viscosityGood moisturizing effectCosmetic preparationsToilet preparationsVitamin CIrritation
The invention relates to anti-dry soft and moist cream (body cream) and a preparation method thereof. The soft and moist cream is characterized by taking pure natural food oats as main raw materials and comprising the following raw materials by weight percent: 30-65% of oat extract, 1-2% of honey, 1-6% of glycerin, 1-5% of beewax, 5-15% of almond oil, 3-15% of emulsifying wax, 0.3-3% of essential oil, 2-8% of vitamin C, 0.1-0.3% of a preservative and the balance of de-ionized water. The soft and moist cream prepared by a formula is milky white cream, fine, uniform, natural and mild, has good nourishing and moisturizing effects, can be used for a long term, can be used for not only skin nourishing and moisturizing but also daily nursing, and has a relatively good effect on dry peptide mass and sensitive skin and the like; the skin allergy and irritation test shows that the anti-dry soft and moist cream (body cream) is non-irritating to rabbit skin and cannot cause allergic reaction to guinea pigs.
Owner:NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
Stable isotope, site-specific mass tagging for protein identification
InactiveUS7125685B2Improve accuracyImprove efficiencyBacteriaSamples introduction/extractionPresent methodProtein-protein complex
Proteolytic peptide mass mapping as measured by mass spectrometry provides an important method for the identification of proteins, which are usually identified by matching the measured and calculated m / z values of the proteolytic peptides. A unique identification is, however, heavily dependent upon the mass accuracy and sequence coverage of the fragment ions generated by peptide ionization. The present invention describes a method for increasing the specificity, accuracy and efficiency of the assignments of particular proteolytic peptides and consequent protein identification, by the incorporation of selected amino acid residue(s) enriched with stable isotope(s) into the protein sequence without the need for ultrahigh instrumental accuracy. Selected amino acid(s) are labeled with 13C / 15N / 2H and incorporated into proteins in a sequence-specific manner during cell culturing. Each of these labeled amino acids carries a defined mass change encoded in its monoisotopic distribution pattern. Through their characteristic patterns, the peptides with mass tag(s) can then be readily distinguished from other peptides in mass spectra. The present method of identifying unique proteins can also be extended to protein complexes and will significantly increase data search specificity, efficiency and accuracy for protein identifications.
Owner:LOS ALAMOS NATIONAL SECURITY
Methods for acquisition and deductive analysis of mixed fragment peptide mass spectra
A method for acquiring and analyzing polypeptide mass spectra comprising: creating first and second sets of fragmented ions using first and second fragmentation techniques; obtaining mass spectra of the first and second sets of fragmented ions; searching a database of fragments of a first fragment pair type and of a second fragment pair type to respectively provide a set of M ranked and a set of N ranked best matching polypeptide sequences; subtracting synthetic spectra corresponding to each of the M and N ranked sequences from the mass spectra to provide first and second sets of modified mass spectra; searching the database of fragments of the second fragment pair type and of the first fragment pair type to provide third and fourth sets of polypeptide sequences providing best matches to the first and second sets of modified spectra, respectively; and creating a cumulative ranking of the third and fourth sets.
Owner:THERMO FINNIGAN
Preparation method for mu-conotoxin
ActiveCN110698553ASolve the dilemma that cannot be synthesized by chemistryHigh purityPeptide preparation methodsAnimals/human peptidesConotoxinDisulfide bonding
The invention relates to the technical field of polypeptide product preparation, in particular to a preparation method for mu-conotoxin. The method disclosed by the invention adopts a solid phase andliquid phase combination method to synthesize a mu-conotoxin product. A solid phase synthesis method is adopted for coupling peptides to resin in sequence from a C terminal to an N terminal accordingto the main chain of the mu-conotoxin, then, the peptides are cut from the resin to obtain a linear peptide, a natural oxidation method is adopted to oxidize the linear peptide to obtain mu-conotoxincrude peptide cyclization liquid with three pairs of disulfide bonds, and the crude peptide cyclization liquid is purified to obtain a high-purity refined peptide. By use of the technology disclosed by the invention, the high levels of aspects, including a refined peptide quality and a total yield, can be simultaneously realized by simple technical steps. Compared with a biological extraction technology, the preparation method disclosed by the invention is simple in reaction operation, short in days for construction, low in cost and high in yields, can easily achieve industrialized requirements and has a wide market prospect.
Owner:应连心
Ginkgo protein GAPIIa and its preparing method and use
The present invention belongs to the field of plant protein separating and purifying technology, and is ginkgo protein GAP IIa separated from gingko and with functions of delaying senility and resisting oxidation and its preparation process and use. The present invention via the process of salt separation, salt dissolution, two-step ion exchanging chromatography and other steps, new protein GAP IIa of molecular weight 29248 Da is separated from gingko and purified. The primary structure and spatial structure detection shows that GAP IIa consists of two peptide chains, GAP IIa-1 and GAP IIa-2, with similar molecular weight and similar amino acid sequence and connected via disulfide bond and with a few glycosyl via O-glycosidic bond. Each of GAP IIa-1 and GAP IIa-2 contains peptide segments of peptide mass number of 911, 1-11 and 2470. The ginkgo protein may be used in producing health food and medicine for resisting oxidation and delaying senility.
Owner:HUAZHONG AGRI UNIV
Identification method of highly stoichiometric non-germline mutation in eucaryotic cell
ActiveCN102140496AHigh stoichiometric ratioMicrobiological testing/measurementMaterial analysis by electric/magnetic meansGermline mutationEucaryotic cell
The invention relates to an identification method of the high stoichiometric ratio non-germline mutation in the eucaryotic cell and provides an semi-quantitative identification method of the protein synthesis error caused by the single amino acid mutation of the non-germline mutation (NGM), wherein the method is based on the proteomics technology and genome DNA sequencing of the mass spectrometry. The method is particularly as follows: the large-scale proteome analysis, the unrestricted sequence alignment and the identification and gene sequencing of the high resolution second-order mass spectrum and third-order mass spectrum of the synthetic peptide segment are integrated to perform systematic synthesis to the NGM and identify that the four peptide segments of four proteins contain the NGMs. By adopting the method for estimation, the NGM occurrence in the peptide segment is about 0.05%, which is more than 0.3% on the protein level. According to the peptide mass spectrum count ratio of mutant peptide segments to normal peptide segments, the stoichiometric ratio of the NGM is 0.05 to 0.71. By adopting the identification method, the NGM is proved to have high occurrence and stoichiometric ratio while the NGM is predicted to be used as a possible mechanism to join the basic biological and physiological processes.
Owner:JINGJIE PTM BIOLAB HANGZHOU CO LTD
In-Gel Tagging And In-Gel Digestion For Phosphoproteins Analysis And Phosphorylation Site Identification
InactiveUS20070287169A1Component separationMicrobiological testing/measurementPhosphopeptideGel electrophoresis
The present invention relates to a method for phosphorylation site-specific labeling of phosphoproteome with a site-specific tagging reagent and analyzing of the resulting labeled one, more especially, a method for in-situ tagging of phosphorylation sites of phosphoproteins retained in polymeric gel with a nucleophilic tagging reagent. It also relates a method for generating new proteolytic cleavable sites at formerly phosphorylation sites by a proper choice of a nucleophilic tagging reagent. It also relates to a method for phosphopeptides analysis and phosphorylation site identification by in-gel digestion of the previously in-gel tagged proteins and subsequent mass analysis of the resulting peptides. The invention provides in-gel chemical tagging method for phosphoaminoacid residue of phosphoproteins retained in polymeric gel matrix. Phosphoprotein can be immobilized into gel matrix by a variety of methods such as gel electrophoresis. The immobilized phosphoproteins are retained in gel matrix during tagging reaction to phosphorylated aminoacid residue of phosphoproteins, and the resulting tagged proteins are also retained in gel matrix till following purification steps like washing of the tagging reagents are accomplished. The tagged proteins is digested by protease, and the resulting digested peptides is released from gel into solution and applied for peptide mass analysis.
Owner:KOREA BASIC SCI INST
Methods for peptide mass spectrometry fragmentation prediction
PendingUS20210041454A1Improve accuracyGood confidenceComponent separationBiological material analysisAdoptive cellular therapyWhite blood cell
The present disclosure relates to methods of improved identification of peptides, for example, antigenic peptides. In particular, the present disclosure relates to methods of more accurately identifying human leukocyte antigen (HLA) peptides by utilizing classification systems. The disclosure also provides for utilizing the described methods for the field of personalized cancer therapies, such as adoptive cellular therapy (ACT).
Owner:IMMATICS US INC +1
Peptide-containing composition and stabilizer, stabilizing method, and storage method for peptide
The present invention provides novel technology for improving the storage stability of peptides such as glutathione. The present invention relates to a peptide-containing composition that comprises apeptide and an alkaline mineral carrier and in which there are 8 parts by mass of more of the alkaline mineral carrier with respect to 100 parts by mass of the peptide. The present invention also relates to a plant cultivation method that includes applying the peptide-containing composition to a plant. The present invention also relates to a stabilizer for a peptide, said stabilizer containing thealkaline mineral carrier. The present invention also relates to a method for improving the stability of a peptide, said method including a mixing step in which the alkaline mineral carrier is mixed with the peptide. The present invention also relates to a method for storing a peptide, said method including a storage step in which the peptide is stored together with the alkaline mineral carrier.
Owner:KANEKA CORP
Collagen zymolyte with blood sugar reducing effect as well as preparation method and application of collagen zymolyte
ActiveCN114349849AImprove oral glucose toleranceRealize enzymatic hydrolysis and controlled releaseConnective tissue peptidesHydrolysed protein ingredientsOral glucoseCollagenan
The invention discloses a collagen zymolyte with a blood sugar reducing effect and a preparation method and application thereof, the zymolyte contains Gly-Pro-type peptide composed of 4-7 amino acid residues, and the mass ratio is 30-45%; the general formula of the sequence of the Gly-Pro-type peptide consisting of 4 to 7 amino acid residues is Gly-Pro-Xaa1-Gly-[Xaa2] r-[Xaa3] s-[Gly] t. According to the preparation method provided by the invention, the zymolyte with strong DPP-IV inhibition activity is prepared for the first time, and the DPP-IV inhibition rate of the zymolyte is as high as 68% when the final concentration is 1mg / mL; the zymolyte is rich in Gly-Pro-type peptide composed of 4-7 amino acid residues, and the peptide yield is as high as 45%; animal experiments prove that the zymolyte can regulate fasting blood-glucose of rats with type 2 diabetes mellitus and improve the oral glucose tolerance of the rats with type 2 diabetes mellitus.
Owner:SOUTH CHINA UNIV OF TECH +1
Methods for large scale protein matching
InactiveUS20030031350A1Time-of-flight spectrometersSamples introduction/extractionMass Spectrometry-Mass SpectrometryData mining
The present invention provides methods for matching a sample of an unknown query peptide to a database of known peptides. The methods described herein allow for the rapid, sensitive, and selective identification of an unknown query peptide, which enables the development of high throughput protein identification. The methods described herein also allow for mass spectrometry data for a query peptide to be categorized and weighted according to its quality. Furthermore, the methods described herein provide robust identification of modified query proteins by either anticipating modifications or adjusting for modified peptide masses.
Owner:APPL BIOSYSTEMS INC
Non-linear fitting mode-based peptide mass spectrum speak characteristic parameter extraction method
InactiveCN104316591AImprove extraction accuracyImproved identification accuracyMaterial analysis by electric/magnetic meansFeature parameterPeptide mass
The invention relates to a non-linear fitting mode-based peptide mass spectrum speak characteristic parameter extraction method. The prior art is difficult to guarantee a precision of the extracted mass spectrum speak characteristic parameter when distributing error of sample points formed in a peptide mass spectrum is large. The non-linear fitting mode-based peptide mass spectrum speak characteristic parameter extraction method is characterized in that data of multiple sample points is utilized, the difference minimum of actual data and a fitting result is used as a guide, and a characteristic parameter estimated value is continuously updated by an iteration method until convergence conditions are satisfies so that the final characteristic parameter estimated value is obtained. The method provided by the invention can effectively reduce the unfavorable influence produced by sample point distributing error on Gauss curve characteristic parameter solving, improve a characteristic parameter value precision and is conducive to peptide segment identification precision improvement.
Owner:HANGZHOU DIANZI UNIV
Method for detecting antipyrine related substances
ActiveCN111855876AEfficient detectionReduce testing costsComponent separationAgainst vector-borne diseasesP phosphateSilica gel
The invention belongs to the field of drug detection, and particularly relates to a method for detecting antipyrine related substances. The detection method comprises the following steps: taking an antipyrine peptide to-be-detected product to prepare a test solution, then taking a phosphate buffer solution as a mobile phase A and an acetonitrile aqueous solution as a mobile phase B to carry out HPLC detection, and taking diphenyl bonded silica gel as a filler for a chromatographic column of the HPLC detection. By establishing the detection method of the antipyrine related substances with highsensitivity, specificity, stability and durability, the quality of the antipyrine can be effectively controlled, and the quality of the antipyrine is stable, controllable and safe.
Owner:BEIJING SAISHENG PHARMA
Glycopeptide analyzer
ActiveUS20210208117A1High similarityImprove the level ofComponent separationMass spectrometric analysisStructure analysisGlycan
A glycopeptide analyzer that performs a structural analysis on glycoforms of a glycoprotein, including: a spectrum creator creating an MS / MS spectrum for each elution time based on data acquired by an LC / MS analysis of a sample containing glycopeptides originating from a target glycoprotein; a peptide mass calculator selecting a glycopeptide-related spectrum from a plurality of MS / MS spectra and calculating the mass of a peptide from the selected spectrum; a similarity determiner determining a similarity between the glycopeptide-related spectrum and each of the other MS / MS spectra; an elution-time range estimator estimating an elution-time range based on a distribution of the frequency of occurrence of an MS / MS spectrum for which a high level of similarity has been determined on a time axis; and a glycan composition estimator selecting an ion peak corresponding to a mass equal to or greater than a peptide mass and estimating a glycan composition based on the peak.
Owner:SHIMADZU CORP
Reagent for cutting sulfydryl-containing peptides from resins and cutting method
ActiveCN101967179BSuitable for industrial productionReduce manufacturing costPeptide preparation methodsEthanedithiolPropanoic acid
Owner:SHANGHAI SOHO YIMING PHARMA
Antimicrobial preparations
ActiveCN110114060BPeptide/protein ingredientsOintment deliveryHerpes simplex virus DNAPharmaceutical drug
The present invention provides a preparation, which relates to the field of medicine, that is, the new pharmaceutical composition has bactericidal, especially, antibacterial and antiviral effects. A preparation of the present invention, containing blowfly antiviral peptide similar drug peptide H 2 N‑His‑Gly‑Val‑Ser‑Gly‑His‑Gly‑Gln‑His‑Gly‑Val‑His‑Gly‑COOH– and peptide HN 2 -(CH 2 ) 10 ‑CO‑Ile‑Leu‑Pro‑D‑Phe‑Lys‑D‑Phe‑Pro‑D‑Phe‑D‑Phe‑Pro‑D‑Phe‑Arg‑Arg‑NH 2 The mass ratio is 1:2 to 2:1 and auxiliary materials. In summary, the preparation has comprehensive antibacterial and antiviral effects, and the preparation (injection, gel, ointment, spray, etc.) can be used to treat herpes virus infection, vaginal herpes infection, HPV virus infection, and bacterial infection.
Owner:长春艾迪尔医用科技发展有限公司
A method for preparing antioxidant peptides by fermenting rice dregs with Bacillus subtilis
ActiveCN104087638BImproves antioxidant activityOptimize composition ratioMicroorganism based processesFermentationAntioxidantRoom temperature
A method for preparing antioxidant peptides by using Bacillus subtilis to ferment rice dregs, the method is to first prepare Bacillus subtilis seed culture solution, then insert it into a fermentation medium, and carry out shaking table fermentation culture to obtain a fermentation solution; Enzyme, cooled to room temperature, centrifuged to remove bacteria and unfermented rice dregs, filtered the obtained fermentation supernatant, concentrated the filtrate, and freeze-dried. The invention uses rice dregs as raw materials to prepare antioxidant peptides, which has wide sources and low price, not only reduces the production cost of preparing antioxidant peptides, but also effectively comprehensively utilizes the by-product rice dregs, increases the added value of rice dregs, and expands its application range. Realize the large-scale industrial production of rice dregs antioxidant peptides. The product rice dregs protein antioxidant peptide prepared by the method of the invention has good quality, high antioxidant activity and strong reducing ability.
Owner:湖南智生源生物科技有限公司
A Method for Extracting Characteristic Parameters of Peptide Mass Spectrum Peaks Based on Nonlinear Fitting Method
InactiveCN104316591BImprove extraction accuracyImproved identification accuracyMaterial analysis by electric/magnetic meansIterative methodFeature parameter
The invention relates to a method for extracting characteristic parameters of peptide mass spectrum peaks. The existing method has the disadvantage that it is difficult to ensure the accuracy of the extracted mass spectrum peak characteristic parameters when there is a large deviation in the distribution of the various points that form the peak in the peptide mass spectrum. The present invention proposes a method for extracting characteristic parameters of peptide mass spectrum peaks based on a nonlinear fitting method, using multiple sample point data, guided by the minimum difference between the actual data and the fitting result, and adopting an iterative method to continuously update the estimated value of the characteristic parameters, Until the convergence condition is satisfied, the final characteristic parameter estimation is obtained. This method can effectively reduce the adverse effects of the sample point distribution deviation on the solution of the characteristic parameters of the Gaussian curve, improve the numerical accuracy of the characteristic parameters, and then help improve the accuracy of peptide identification.
Owner:HANGZHOU DIANZI UNIV
Test of amino acid sequence constituting peptide using isotopic ratio
InactiveUS20090012714A1Biological testingSequence analysisMedicineMass Spectrometry-Mass Spectrometry
It is an object of the present invention, when determining and identifying an amino acid sequence of a peptide using MS, to obtain additional information from the MS for evaluating validity of an amino acid sequence in a candidate list outputted from an identifying engine. The present invention provides a method of testing an amino acid sequence inferred by searching a peptide-related database based on peptide mass information and / or peptide modification information obtained through mass spectrometry on a peptide, the method comprising the steps: (1) calculating a theoretical value of an isotopic ratio for the peptide from the inferred amino acid sequence and / or the peptide modification information; (2) measuring a measured value of the isotopic ratio for the peptide from the peptide mass information; and (3) comparing the theoretical value and the measured value, and evaluating validity of the inferred amino acid sequence from differences between the theoretical value and the measured value.
Owner:EISIA R&D MANAGEMENT CO LTD
A rapid identification method for cross-linked dipeptides
ActiveCN106033501BFast searchImprove identification efficiencySpecial data processing applicationsCross-linkDipeptide
The invention provides a crosslinking dipeptide rapid identification method, comprising: 1) extracting effective spectrum peaks in a to-be-identified series-connected spectrogram, according to mass corresponding to each effective spectrum peak, searching a fragment index, to obtain a corresponding peptide fragment sequence as a candidate [alpha] peptide sequence, wherein the fragment index records mass of each fragment and the peptide fragment sequence corresponding to the mass of each fragment; 2) for each candidate [alpha] peptide sequence, according to parent ion mass of the to-be-identified series-connected spectrogram, calculating corresponding [beta] peptide mass, to obtain a corresponding candidate [beta] peptide sequence, combining the candidate [alpha] peptide sequence with the corresponding candidate [beta] peptide sequence, to obtain candidate crosslinking dipeptide; 3) matching the candidate crosslinking dipeptide obtained in the step 2) with the series-connected spectrogram in a fine manner, to obtain an identification result. The method does not need to use a special cross-linking agent, searching speed is fast, identification efficiency is high, and search sensitivity is high.
Owner:INST OF COMPUTING TECH CHINESE ACAD OF SCI +1
Ginkgo protein GAPIIa and its preparing method and use
InactiveCN1218960CExplore biological activityAnti agingAntinoxious agentsPeptide preparation methodsIon exchangeDissolution
The present invention belongs to the field of plant protein separating and purifying technology, and is ginkgo protein GAP IIa separated from gingko and with functions of delaying senility and resisting oxidation and its preparation process and use. The present invention via the process of salt separation, salt dissolution, two-step ion exchanging chromatography and other steps, new protein GAP IIa of molecular weight 29248 Da is separated from gingko and purified. The primary structure and spatial structure detection shows that GAP IIa consists of two peptide chains, GAP IIa-1 and GAP IIa-2, with similar molecular weight and similar amino acid sequence and connected via disulfide bond and with a few glycosyl via O-glycosidic bond. Each of GAP IIa-1 and GAP IIa-2 contains peptide segments of peptide mass number of 911, 1-11 and 2470. The ginkgo protein may be used in producing health food and medicine for resisting oxidation and delaying senility.
Owner:HUAZHONG AGRI UNIV
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