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Antigen protein for treating Alzheimer's disease and protein gene

A technology of recombinant antigen protein and fusion gene, applied in the field of immunity, can solve the problems of poor immunogenicity and achieve the effect of enhancing immunogenicity and improving safety

Inactive Publication Date: 2012-05-30
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the use of full-length Aβ42 as an immunogen is potentially dangerous, because Aβ42 itself is a pathogenic factor, and as a small peptide, Aβ42 has a molecular weight of less than 10,000 and has poor immunogenicity

Method used

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  • Antigen protein for treating Alzheimer's disease and protein gene
  • Antigen protein for treating Alzheimer's disease and protein gene
  • Antigen protein for treating Alzheimer's disease and protein gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Preparation of recombinant antigenic protein GST-I-Aβ28, comprising the following steps:

[0043] (1) Design the recombinant antigenic protein of the present invention

[0044] The design of the recombinant antigen protein GST-I-Aβ28 of the present invention makes its complete amino acid sequence as shown in SEQ ID NO: 7, which includes a sequence of SEQ ID NO: 4 (GST), and a sequence of SEQ ID NO: 5 ( I) sequence and 1 sequence of SEQ ID NO: 2 (Aβ28), 2 immune enhancement sequences of SEQ ID NO: 4 and SEQ ID NO: 5 after internal recombination, and then with 1 human β-amyloid peptide sequence SEQ ID NO : 2-phase coupling to form a recombinant protein; the recombinant SEQ ID NO: 4 (GST) sequence is not only a part of the recombinant antigen protein, but also a purification tag for the expressed protein.

[0045] (2) Synthesize the gene encoding GST-I-Aβ28 and construct the recombinant expression vector

[0046] According to the amino acid sequence of the ...

Embodiment 2

[0059] Embodiment 2: Preparation of recombinant antigenic protein GST-Aβ35, comprising the following steps:

[0060] (1) Design the recombinant antigenic protein of the present invention

[0061] The design of the recombinant antigen GST-Aβ35 of the present invention makes its complete amino acid sequence as shown in SEQ ID NO: 8, which includes a sequence of SEQ ID NO: 4 (GST) and a sequence of SEQ ID NO: 3 (Aβ35) . The recombinant SEQID NO: 4 (GST) sequence is not only a part of the recombinant antigen protein, but also a purification tag for the expressed protein.

[0062] (2) Synthesize the gene encoding GST-Aβ35 and construct the recombinant expression vector

[0063] According to the amino acid sequence of the recombinant antigenic protein GST-Aβ35 designed in the present invention, a gene fragment encoding Aβ35 was designed and artificially synthesized using a DNA synthesizer, and its sequence is as follows:

[0064] cg ggatcc gatgcagaattccgtcatgactcaggatatgaagttca...

Embodiment 3

[0073] Embodiment 3: Preparation of recombinant antigenic protein GST-(Aβ9-K6)9-Aβ9, comprising the following steps:

[0074] (1) Design the recombinant antigenic protein of the present invention

[0075] The design of the recombinant antigen GST-(Aβ9-K6)9-Aβ9 of the present invention makes its complete amino acid sequence as shown in SEQ ID NO: 9, which includes 1 sequence of SEQ ID NO: 4 (GST) and 10 sequences of SEQ ID NO : 1 (Aβ9) sequence and 9 sequences of SEQ ID NO: 6 (K6). The recombinant SEQ ID NO: 4 (GST) sequence is both a part of the recombinant antigenic protein and a purification tag for the expressed protein.

[0076] (2) Synthesize the gene encoding GST-(Aβ9-K6)9-Aβ9 and construct the recombinant expression vector

[0077] According to the amino acid sequence of the recombinant antigen protein GST-(Aβ9-K6)9-Aβ9 designed in the present invention, a gene fragment encoding (Aβ9-K6)9-Aβ9 was designed and artificially synthesized using a DNA synthesizer, and its s...

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Abstract

The invention belongs to the immune technology field, in particular relates to a recombinant antigen protein which is used for preventing and treating the Alzheimer disease (senile dementia) and a gene for encoding the recombinant antigen protein. The recombinant antigen protein comprises 1-20 immune enhancement sequences and 1-20 human Beta-amyloid peptide sequences, wherein, the Beta-amyloid peptide sequences have the amino acid sequences which are shown by SEQ ID NO: 1.SEQ ID NO: 2. or SEQ ID NO: 3; and the immune enhancement sequences are selected from SEQ ID NO: 4.SEQ ID NO: 5. or SEQ IDNO: 6. sequences, or the amino acid sequences which have at least 90% of homology with the sequences. The recombinant antigen protein not only removes the toxic fragment at C-terminal of the full-length Amyloid Beta, improves the safety of the Amyloid Beta, but also recombines the immune enhancement sequences and enhances the immunogenicity of the Amyloid Beta.

Description

technical field [0001] The invention belongs to the technical field of immunization, and specifically relates to a recombinant antigenic protein for preventing and treating Alzheimer's disease (senile dementia) and a fusion gene encoding the recombinant antigenic protein. Background technique [0002] Alzheimer's disease (Alzheimer disease, AD), also known as senile dementia, is a neurodegenerative disease, which is clinically manifested as dementia with mental symptoms: such as memory loss or even loss, cognitive impairment and behavioral slowness Even incapacity etc. The main pathological feature of AD is the appearance of neurotoxic β-amyloid peptide (β-amyloid peptide, Aβ or Aβ42) composed of 42 amino acids outside the neurons in the patient's brain. For its amino acid sequence, see Strooper BD, et al .An inflammatory drug prospect.Nature.2001, 64(6860):159-160) aggregates and amyloid plaques further formed therefrom, causing neurofibrillary tangles and apoptosis of bra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/63A61P25/28
Inventor 张应玖黄雪媚崔理立
Owner JILIN UNIV