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Genetically modified plants which synthesize a starch havingincreased swelling power

A genetic modification and plant technology, applied in the field of genetically modified plant cells and plants, can solve the problem of not being able to obtain a significant increase

Inactive Publication Date: 2009-07-29
BAYER INTELLECTUAL PROPERTY GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be concluded that the swelling property of starch has not been significantly increased by the current conventional derivatization method, even if it is increased.

Method used

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  • Genetically modified plants which synthesize a starch havingincreased swelling power
  • Genetically modified plants which synthesize a starch havingincreased swelling power
  • Genetically modified plants which synthesize a starch havingincreased swelling power

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Experimental program
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Effect test

Embodiment Construction

[0317] 1. Preparation of the plant expression vector AH32-191 comprising the coding sequence of the protein with starch synthase II activity

[0318] Cut out the full-length coding sequence of the protein (T.a.-SSII) with starch synthase II activity from wheat from plasmid pCF31 (described in WO 9745545, called pTaSS1) by restriction endonuclease Ecl136ll and Xho I, then This was cloned into plasmid IR103-123 (described in WO 05 030941 ) cut with restriction endonucleases EcoRV and Xho I. The obtained expression vector was called AH32-191. The plant expression vector IR103-123 was used for endosperm-specific expression of the target gene under the control of a globin promoter from rice. The plant expression vector IR103-123 also contained the bar gene under the control of the CaMV35S promoter, which was used as a selectable marker for plant transformation.

[0319] 2. Production of rice plants in which the activity of a protein having starch synthase II activity is increased...

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Abstract

The present invention relates to genetically modified plant cells and plants, and to processes for the production of genetically modified plant cells and plants which have an increased activity of a protein having the activity of a starch synthase II and an increased activity of a protein having the activity of a glucan-water dikinase. The plants synthesize starches having increased hot water swelling capacity. The present invention likewise relates to starches having increased hot water swelling capacity, and to processes for their production.

Description

technical field [0001] The present invention relates to genetically modified plant cells and plants, to methods for producing genetically modified plant cells and plants in which the activity of a protein having starch synthase II activity is increased and has dextran-water Increased activity of proteins with dikinase (glucan-water dikinase) activity. Such plants synthesize starches with increased hot water swelling. The present invention also relates to starches with increased hot water swellability and methods for their preparation. Background technique [0002] Besides oils, fats and proteins, polysaccharides are the main renewable raw materials in plants. Besides cellulose, starch occupies a central position among polysaccharides as one of the most important storage substances in higher plants. [0003] Furthermore, starch is an essential component of human and animal nutrition in terms of nutritional physiology. The structural characteristics of starches contained i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10A23L1/0522C12N15/29C12N9/12A01H5/00C12N9/10C08B30/04
CPCC08B30/044C12N15/8245C08B31/00C08B30/042C08B30/04C12N9/1051C12N9/1294
Inventor C·弗罗贝格
Owner BAYER INTELLECTUAL PROPERTY GMBH