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Detectable Nucleic Acid Tags

A nucleic acid and nucleic acid sequence technology, applied in the directions of labels, measurement devices, and biological tests used in chemical analysis, which can solve the problems of difficulty in high-throughput applications, cumbersomeness, detection sensitivity and specificity limitations, etc.

Active Publication Date: 2016-08-03
迪斯卡沃雷克斯公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Each of these methods is cumbersome and difficult to adapt for high-throughput use
These traditional methods also have limitations in detection sensitivity and specificity

Method used

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  • Detectable Nucleic Acid Tags
  • Detectable Nucleic Acid Tags
  • Detectable Nucleic Acid Tags

Examples

Experimental program
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Embodiment

[0084] Unless otherwise indicated, the practice of the systems and methods provided herein employs molecular biology, microbiology, genetic analysis, recombinant DNA, organic chemistry, biochemistry, PCR, oligonucleotide synthesis and modification, nucleic acid hybridization, and techniques in the art Traditional techniques in the relevant field mastered by the skilled person. These techniques are described in the references cited herein and explained in detail in the literature. See eg: Maniatis et al., (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press; Sambrook et al., (2001), Molecular Cloning: A Laboratory Manual. Third Edition, ColdSpring Harbor Laboratory Press; Ausubel et al., Current Protocols in Molecular Biology. John Wiley & Sons (1987 and updated annually); Gait (editor) (1984) Oligonucleotide Synthesis: APractical Approach. IRL Press; Eckstein (ed.) (1991) Oligonucleotides and Analogues: A Practical Approach. IRLPres...

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PUM

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Abstract

The present invention provides a nucleic acid tag linked to, or capable of linking to, a protein of interest. Specifically, the nucleic acid label is an oligonucleotide comprising a reporter function and a protein labeling function. The invention also provides nucleic acid label composition, kit and using method thereof.

Description

field of invention [0001] The subject matter provided by the present invention relates to nucleic acid tags linked to, or capable of linking to, a protein of interest. In particular, the subject matter provided by the present invention relates to oligonucleotides comprising a reporter function and a protein labeling function. The invention also provides nucleic acid label composition, kit and using method thereof. Background of the invention [0002] Traditional techniques to quantify and detect the presence of proteins include gel electrophoresis, Western blotting, ELISA-based immunosorbent assays, and protein microarrays. Each of these methods is cumbersome and difficult to adapt for high-throughput use. These traditional methods also have limitations in detection sensitivity and specificity. The present invention provides a nucleic acid tag, and a novel, highly sensitive and selective protein detection method using the nucleic acid tag. Brief description of the inven...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/62G01N33/68
CPCC12Q1/68G01N33/585G01N33/532C12Q2563/179C12Q2522/101C12Q2563/113C12Q2563/101C12Q1/6806G01N33/573G01N2458/00
Inventor 皮艾特罗·奇切里杰里米·亨特吉恩-迈克尔·A·莱利亚斯迈克·莫里森丹尼尔·特莱柏利萨·M·沃迪科卡
Owner 迪斯卡沃雷克斯公司