Method for immunoassay by utilizing zinc oxide quantum dots

An immunoassay and quantum dot technology, which is applied to the electrochemical variables of materials, material analysis through electromagnetic means, and measurement devices, can solve the problems of delaying disease discovery and optimal treatment timing, long cycle, and poor accuracy. The effect of shortening the detection time, low detection limit, and lower detection limit

Inactive Publication Date: 2009-09-09
JIANGSU HONGSHUN SYNTHETIC FIBER TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The limitation of this method lies in its long period and poor accuracy, which may delay the discovery of the disease and the best time for treatment

Method used

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  • Method for immunoassay by utilizing zinc oxide quantum dots
  • Method for immunoassay by utilizing zinc oxide quantum dots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step 1: Burn one end of a gold wire or platinum wire to form a small ball, then vapor-deposit a layer of zinc with a thickness of about 200 nanometers on the surface of the ball, put it in a tube furnace for oxidation at about 300°C for 1 hour, and take it out Boil with 2M KOH for 2 hours, take it out, wash it with deionized water, and place it in a place containing 30% (volume ratio) H 2 o 2 H 2 SO 4 After 30 minutes, it was taken out, washed with deionized water, and dried at room temperature.

[0029] The second step: mix the zinc powder and deionized water for 20 minutes, let it stand for 30 minutes, pour off the water, transfer the zinc powder and water to the autoclave according to 1:100 (mass ratio), and let it stand until there is no suspension Granules, the gold or platinum treated in the first step is immersed in water without touching the zinc powder at the bottom of the autoclave. Sealed so that it can withstand a maximum pressure of not less than 1.5×10...

Embodiment 2

[0038] Step 1: Melt one end of a gold wire with a diameter of 0.5 mm into a small ball with a diameter of about 1.2 mm, evaporate a layer of zinc with a thickness of about 200 nanometers on the surface of the ball, put it in a tube furnace at 300 ° C to oxidize 1 hours, take it out and boil it with 2M KOH for 2 hours, take it out and wash it with deionized water, and then place it in a place containing 30% H 2 o 2 H 2 SO 4 (volume ratio) after 30 minutes, take it out, and then wash it with deionized water.

[0039] The second step: mix the zinc powder and deionized water for 20 minutes, let it stand for 30 minutes, pour off the water, transfer the zinc powder and water to the autoclave according to 1:100 (mass ratio), and let it stand until there is no suspension Granules, the gold wire treated in the first step is immersed in water without touching the zinc powder at the bottom of the autoclave. Sealed so that it can withstand a maximum pressure of not less than 1.5×10 5...

Embodiment 3

[0048] Step 1: Melt one end of a gold wire with a diameter of 0.5 mm into a small ball with a diameter of about 1.2 mm, evaporate a layer of zinc with a thickness of about 200 nanometers on the surface of the ball, put it in a tube furnace at 300 ° C to oxidize 1 hours, take it out and boil it with 2M KOH for 2 hours, take it out and wash it with deionized water, and then place it in a place containing 30% H 2 o 2 H 2 SO 4 (volume ratio) after 30 minutes, take it out, and then wash it with deionized water.

[0049] The second step: mix the zinc powder and deionized water for 20 minutes, let it stand for 30 minutes, pour off the water, transfer the zinc powder and water to the autoclave according to 1:100 (mass ratio), and let it stand until there is no suspension Granules, immerse the gold wire treated in the first step in water without touching the zinc powder at the bottom. Seal and heat to 90°C for 12 hours. Cool naturally to room temperature, take it out, and wash it ...

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Abstract

The invention relates to a method immunoassay by utilizing zinc oxide quantum dots. Horse radish peroxidase is assembled on an upper layer of quantum dots and then an antibody is assembled on an outer layer. Meanwhile, a zinc oxide nanometer rod grows on a golden electrode, andan antibody is also assembled on the zinc oxide nanometer rod. An antigen to be measured and the antibody on the quantum dots are combined with the antibody on the zinc oxide nanometer rod through specific combination of the antibody and the antigen, a very small amount of antigens is used for connecting the quantum dots assembled with a large amount of horse radish peroxidase to the golden electrode of the zinc oxide nanometer rod, electrochemical response of catalytic action of the horse radish peroxidase to peroxide is detected, and then the relationship of antigen concentration and electrochemical signals of catalytic action of the horse radish peroxidase to peroxide is established. The detection method makes antigen with very low concentration generate obvious electrochemical catalytic signals, and plays a role of signal amplification. The invention has important implications in the aspects of early detection of diseases in medical field.

Description

1. Technical field [0001] The invention belongs to the technical field of immunoassay, and in particular relates to an immunoassay method using zinc oxide quantum dots. 2. Background technology [0002] In addition to its unique semiconductor photoelectric properties, nanostructured zinc oxide is also biologically safe and non-toxic, has excellent biocompatibility, can chelate with proteins and other biomolecules by hydrogen bonds or other methods, and can well maintain its biological activity. , have excellent properties such as high isoelectric point, which makes it have important applications in biological / chemical sensors. [0003] At present, the detection of low-concentration antigens is an important issue in clinical medicine and diagnostics. Successful detection of low-concentration antigens can provide scientific basis for early diagnosis and treatment of corresponding diseases. The traditional method of antigen concentration detection is to first culture the viru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/551G01N27/26G01N27/327
Inventor 谷保祥徐春祥刘松琴陈丽媛朱光平
Owner JIANGSU HONGSHUN SYNTHETIC FIBER TECH
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