Transfer factor solution for livestock and poultry, and preparation method thereof

Abstract

The invention provides a transfer factor solution for livestock and poultry. Frozen pig spleens are washed clean by use of distilled water, cut into small pieces, added with distilled water free from heat source, subjected to cell disruption by use of a tissue bruiser and prepared into homogenate; the homogenate passes through a high-pressure homogenizer under the pressure of 60 kPa and then is added with a flocculating agent for flocculation; the flocculated homogenate is centrifuged at a centrifugation ambient temperature of 4 DEG C and at a speed of 7,000 r / min; precipitate is removed; supernatant is left on standby; the supernatant is filtered by use of a microporous filtering membrane 0.45 mu m in pore diameter, and then is ultrafiltered by use of a ultrafiltration membrane 5,000 Dalton in molecular weight cutoff so as to obtain a stock solution; after viruses are removed, nicotinamide is added as a stabilizer; the stock solution is subjected to aseptic filtration by use of the microporous filtering membrane; and the obtained product is added with glycerol as a heat-resisting protective agent and then is directly and separately packed. The transfer factor solution can be applied to livestock and poultry. The preparation method is characterized by directly using the pig spleen to extract the transfer factor solution so as to save manpower and materials and improve extraction efficiency. The preparation method combines a heat-resisting protection technique with transfer factors, thereby realizing the normal-temperature preservation of the transfer factors. The transfer factor solution can improve the disease-resisting capability of organisms, relieve immunologic suppression and immunologic tolerance, can play a role in emergency adjuvant treatment, and makes up for immunization blank after vaccination before antibody production.

Description

technical field [0001] The invention belongs to a transfer factor used to improve the immunity of livestock and poultry in the technical field of bioengineering, and particularly relates to a transfer factor solution applied to livestock and poultry, a preparation method thereof and an application to livestock and poultry. Background technique [0002] With the continuous improvement of the scale and intensification of modern farming, viral diseases have become a major problem for farmers. The existing general solution is immunization. However, due to various reasons, sometimes it is inevitable that immunization failure will occur, and atypical infectious diseases will break out, which will bring certain difficulties to diagnosis and treatment. After immunization failure, they often rely on antibiotic treatment, resulting in drug resistance. Increase in strains and generation of cross-infection. As a cellular immune enhancer, transfer factor can play an important role in th...

AI Technical Summary

Problems solved by technology

At present, the existing transfer factor solution preparation technology is usually to extract the fat and large blood vessels in the spleen of animals, and then perform a series of procedures such as breaking the wall, freeze-thawing and dialysis. Although this method is simple, it often requires a lot of manpower and material resources, and the transfer factor solution prepared by this method can only be stored at -15°C, which brings great inconvenience to use and transportation