Achromobacter xylosoxidans strain for biological denitrificaion and application thereof
An achromobacter, biological denitrification technology, applied in the field of biological denitrification, can solve problems such as long process, achieve the effect of broad application prospect, simplified process flow and good social benefit
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Embodiment 1
[0037] Example 1. Obtainment of strain GAD3 with heterotrophic nitrification-aerobic denitrification performance and biological denitrification experiment
[0038] 1. Separation and purification method of strain GAD3 with heterotrophic nitrification-aerobic denitrification performance
[0039] The method has the following steps:
[0040] 1) Take out sludge samples from the combined treatment system of landfill leachate in Shenzhen Buji landfill;
[0041] 2) Inoculate into a 500mL Erlenmeyer flask containing 100mL enriched culture solution: each L contains 0.95g NaNO 3 , 0.7g peptone, 0.5g beef extract, 0.15g urea, 0.04g NaCl, 0.15g KH 2 PO 4 , 0.02g KCl, 0.03g MgSO 4 ·7H 2 O, 0.20g CaCl 2 ·2H 2 O, pH 7.0-7.5;
[0042] 3) Wrap the mouth of the bottle with gauze, and culture for 4 days at 30°C and 140 rpm shaker;
[0043] 4) Take the bacterial solution in the shake flask as the source of bacterial seed, take out 10mL and inoculate it into a fresh conical flask containing 150mL enriched cul...
Embodiment 2
[0062] Example 2. Biological Denitrification Experiment
[0063] The strain GAD3 and its bacterial suspension prepared by the method provided in Example 1 were used for biological denitrification experiments.
[0064] 1. Take ammonia nitrogen (NH 4 + -N) Biological nitrogen removal experiment with nitrogen source
[0065] Take 5mL of the prepared GAD3 bacterial suspension and add it to three culture flasks containing 95ml test medium (each L medium contains 1.52g NH 4 Cl, 1.0g KH 2 PO 4 , 0.06g FeSO 4 ·7H 2 O, 0.2gCaCl 2 ·2H 2 O, 1.0g MgSO 4 ·7H 2 O, 8.1g sodium citrate, pH 8.0), sealed with 9 layers of gauze, 15°C, 160rpm (rotation radius 15mm) shake culture in a shaker. The medium without bacterial suspension was subjected to the experiment under the same conditions as a blank control. The reaction solution was taken the next day and centrifuged at 4000 rpm for 10 min. The supernatant was taken to determine the concentration of various nitrogen-containing compounds.
[0066] The re...
Embodiment 3
[0071] Example 3. Experiment of biological denitrification conditions
[0072] The strain GAD3 and its bacterial suspension prepared by the method provided in Example 1 were subjected to biological denitrification condition experiments.
[0073] 1. Take ammonia nitrogen (NH 4 + -N) Biological denitrification condition experiment with nitrogen source
[0074] Take 5mL of the prepared GAD3 bacterial suspension and add it to 9 culture flasks containing 95ml test medium (each L medium contains 0.57g NH 4 Cl, 1.0g KH 2 PO 4 , 0.06g FeSO 4 ·7H 2 O, 0.2gCaCl 2 ·2H 2 O, 1.0g MgSO 4 ·7H 2 O, 7.72g anhydrous sodium acetate), the initial pH is 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, 11.0, respectively. Seal with 9 layers of gauze, shake culture in a shaker at 40°C and 160 rpm (rotation radius of 15 mm). The medium without bacterial suspension was used as a blank control under the same conditions. After culturing for 24 h, take the reaction solution in the shake flask, centrifuge at 4000 rpm for 10 min...
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