Cascaded control algorithm of gene amplification equipment

Abstract

The invention provides a cascaded control algorithm of gene amplification equipment. The gene amplification equipment comprises a temperature conversion module, two controllers, an internal procedure processing module, two sensors, two filters, a DA digital / analog conversion module, a drive module and a plurality of variables. The temperature conversion module takes charge of converting a decimal centigrade counter format for a set value of temperature of a user into a data format the same as the format of output of an analog / digital conversion module. The cascaded control algorithm is a control algorithm consisting of two PID controllers, wherein, the output of the external controller is a setting point of an internal controller; the external controller helps compare difference between the set value and a measured value by the sensor and drives execution element to eliminate the difference according to the control algorithm; the internal environment temperature and the external environment temperature are major interference during gene amplification, the interference exists in a quick dropping phase of a temperature control flow especially, and the internal controller detects for the external interference and supplements according to the control algorithm before the external interference affects the system.

Description

technical field [0001] The invention belongs to the detection field of biology and medicine, and in particular relates to a cascade control algorithm of gene amplification equipment. Background technique [0002] In the control process of traditional gene amplification, temperature process control is a difficult point. The temperature sensing subsystem measures the temperature change of the control process, and the feedback mechanism of the system sensing signal and control process will cause a delay in the system response speed. This will cause overshoot or lack of system temperature indicators. In this case, the set point and actual temperature will have errors, which will take a long time to obtain a stable effect; a gene amplification device that requires a fast steady state It is difficult to control the temperature control system with a single controller, because the heat energy will quickly accumulate and oscillate in the small space of the equipment. If it is control...

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Problems solved by technology

[0002] In the control process of traditional gene amplification, temperature process control is a difficult point. The temperature sensing subsystem measures the temperature change of the control process, and the feedback mechanism of the system sensing signal and control process will cause a delay in the system response speed. This will cause overshoot or lack of system temperature indicators. In this case, the set point and actual temperature will have errors, which will take a long time to obtain a stable effect; a gene amplification device that requires a fast steady state It is difficult to control the temperature control system with a single controller, because the heat energy will quickly accumulate and oscillate in the small space of the equipment. If it is controlled with a single controller, it will take a lot of time to obtain a stable process, and gene amplification requires The temperature rises and falls quickly and maintains a stable state quickly. Therefore, the traditional single feedback control obviously cannot satisfy the amplification mechanism and growth law of the gene. The cascade cascade control strategy will be used in the control system of the gene amplification equipment

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