Special electrode for in-vivo voltammetry analysis and preparation method thereof

A micro-electrode and potential technology, which is used in the analysis of materials, material analysis by electromagnetic means, and electrochemical variables of materials, etc., to achieve good stability, low detection limit, and easy reproducibility.

Inactive Publication Date: 2013-01-23
INST OF CHEM CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the established methods can meet the real-time monitoring of some physiologically active substances in vivo, there are still great challenges in the research of microelectrode in vivo voltammetry analysis methods, mainly reflected in the preparation and methods of electrodes that can meet the purpose of in vivo voltammetry analysis. reproducibility and stability

Method used

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  • Special electrode for in-vivo voltammetry analysis and preparation method thereof
  • Special electrode for in-vivo voltammetry analysis and preparation method thereof
  • Special electrode for in-vivo voltammetry analysis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Preparation of carbon fibers covered with carbon nanomaterials

[0028] Carbon fiber (T650-35, 7 μm diameter) (Fabric Development Inc.). The carbon fiber is placed in a high temperature furnace of 1100 ° C, and the SiCl 4 The mixed gas flow of 300mL / min argon and 40mL / min hydrogen was injected into the high temperature furnace containing atmospheric oxygen for 20min. 4 Reaction with carbon fiber to generate SiO on the surface of carbon fiber 2 thin layer. The prepared carbon fibers and 0.1-0.3 g of iron phthalocyanine (FeC 32 N 8 H 16 ) were placed in the same quartz glass tube, in Ar / H 2 (The volume ratio is 1:1~1:2, and the flow rate is 20~40cm 3 / min), different heat treatments were performed for carbon fibers and iron phthalocyanine. For the heat treatment of carbon fibers, the temperature is 800-1100°C for 2-15 minutes, and for the heat treatment of iron phthalocyanine, the temperature is 500-600°C for 2-15 minutes. Then, the temperature of both...

Embodiment 2

[0029] Example 2. Preparation of carbon fiber microelectrodes covered with carbon nanomaterials

[0030] Micro-adhere the 1-2cm carbon fiber covered with carbon nanomaterials on the surface of Example 1 to 5-10cm copper wire with conductive glue, and insert it into the drawn glass capillary with both ends open (inner diameter is 1.5 cm). mm, the length is 10cm), the inner diameter of the thin end opening is 30-50μm, and the length of the carbon fiber exposed at this end is about 1mm. The thick end of the glass tube exposes the copper wire. After the openings at both ends of the glass tube were sealed with insulating glue, the prepared electrodes were dried at 100° C. for 1 h to obtain carbon fiber microelectrodes covered with carbon nanomaterials on the surface.

Embodiment 3

[0031] Embodiment 3. Preparation method one of special electrodes for in vivo voltammetry analysis of physiologically active substances

[0032] 1) The carbon fiber electrodes with carbon nanomaterials on the surface prepared in Example 2 were prepared in acetone, ethanol, and 1.0-3.0M HNO in sequence. 3 , 1.0~2.0M KOH solution and ultrasonic in secondary water. Ultrasonic intensity is 20W / cm 2 , the ultrasonic frequency is 50kHz, the ultrasonic action mode is 20s of ultrasonic work every 15s, and the ultrasonic time is 2 to 5min;

[0033] 2) Electrochemically activate the carbon fiber electrode in step 1) in a 0.5M sulfuric acid solution, that is, firstly apply a potential polarization of +2.0V to the electrode for 30-50s, and then apply a potential polarization of -1.0V for 10-30s. The obtained electrode is then subjected to 0.05-0.2Vs -1 The scanning speed is cyclically scanned in the potential range of 0.0-1.0V until a stable cyclic voltammogram is obtained. The schema...

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Abstract

The invention discloses a special electrode for in-vivo voltammetry analysis and a preparation method thereof. The preparation method of the special electrode comprises the following steps: 1) preparing carbon fiber coated with a carbon nanomaterial on surface; 2) preparing a carbon fiber microelectrode; 3) soaking the carbon fiber electrode prepared by the step 2) in solution for ultrasonic washing; and 4) subjecting the carbon fiber electrode prepared by the step 3) to electrochemical pretreatment to obtain the special electrode. Meanwhile, an electrochemical detection device for in vitro correction or in vivo real-time physiological activator monitoring is prepared by using the special electrode. The electrochemical detection device comprises the special electrode for physiological activator monitoring of the invention, a reference electrode and an auxiliary electrode. The electrochemical detection device prepared by using the special electrode of the invention can meet the requirements for the in-vivo voltammetry analysis of physiological activators and has a promising application prospect in researches in fields of neurophysiology, clinical medicine and the like.

Description

technical field [0001] The invention relates to a special electrode capable of satisfying the in vivo voltammetry analysis of physiologically active substances and a preparation method thereof. Background technique [0002] In recent years, the research on the change law of chemical substances in physiological and pathological processes has become a frontier issue of widespread concern, mainly because the real-time changes of chemical substances in vivo can directly reflect the chemical nature of physiological and pathological processes. For example, the changes of glucose and lactate in the brain can directly reflect the energy metabolism process in the brain; the existence level of the neurotransmitter dopamine in the brain and its change rule are directly related to important physiological phenomena such as physical movement and mental activity; the existence of ascorbic acid in the brain The level is closely related to the oxidative damage of free radicals and the synthe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/48
Inventor 毛兰群向玲戴黎明
Owner INST OF CHEM CHINESE ACAD OF SCI
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