Special electrode for in-vivo voltammetry analysis and preparation method thereof

A micro-electrode and potential technology, which is applied in the analysis of materials, material analysis by electromagnetic means, and electrochemical variables of materials, etc., to achieve wide application prospects, good stability, and high selectivity.

Inactive Publication Date: 2009-11-18
INST OF CHEM CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the established methods can meet the real-time monitoring of some physiologically active substances in vivo, there are still great challenges in the research of microelectrode in vivo voltammetry analysis methods, mainly reflected in the preparation and methods of electrodes that can meet the purpose of in vivo voltammetry analysis. reproducibility and stability

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  • Special electrode for in-vivo voltammetry analysis and preparation method thereof
  • Special electrode for in-vivo voltammetry analysis and preparation method thereof
  • Special electrode for in-vivo voltammetry analysis and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1, the preparation of the carbon fiber of surface covering carbon nanomaterial

[0028] Carbon fiber (T650-35, 7 μm in diameter) (Fabric Development Inc.). Put the carbon fiber into a high-temperature furnace at 1100 ° C, and put SiCl 4 Inject a mixed flow of 300mL / min argon and 40mL / min hydrogen into a high-temperature furnace containing oxygen at atmospheric pressure for 20 minutes. 4 Reacts with carbon fibers to generate SiO on the surface of carbon fibers 2 TLC. The prepared carbon fiber and 0.1 ~ 0.3g iron phthalocyanine (FeC 32 N 8 h 16 ) respectively placed in the same quartz glass tube, under Ar / H 2 (The volume ratio is 1:1~1:2, the flow rate is 20~40cm 3 / min) atmosphere, different heat treatments were carried out for carbon fiber and iron phthalocyanine. For the heat treatment of carbon fiber, it is heated at a temperature of 800-1100°C for 2-15 minutes, and for the heat treatment of iron phthalocyanine, it is heated at a temperature of 500-...

Embodiment 2

[0029] Embodiment 2, the preparation of the carbon fiber microelectrode of surface covering carbon nanometer material

[0030] The carbon fiber of the surface covering carbon nanomaterial of 1~2cm of embodiment 1 is micro-adhered on the copper wire of 5~10cm with conductive glue, and it is penetrated in the glass capillary tube (internal diameter is 1.5 mm, the length is 10cm), the inner diameter of the thin end opening is 30-50μm, and the length of the exposed carbon fiber at this end is about 1mm. The thick end of the glass tube exposes the copper wire. After the openings at both ends of the glass tube were sealed with insulating glue, the prepared electrode was baked at 100° C. for 1 hour to obtain a carbon fiber microelectrode covered with carbon nanomaterials.

Embodiment 3

[0031] Example 3. Preparation Method 1 of Special Electrode for Vivo Voltammetric Analysis of Physiologically Active Substances

[0032] 1) The carbon fiber electrode whose surface is covered with carbon nanomaterials prepared in Example 2 is successively dissolved in acetone, ethanol, 1.0-3.0M HNO 3 , 1.0~2.0M KOH solution and ultrasonic in secondary water. The ultrasonic intensity is 20W / cm 2 , the ultrasonic frequency is 50kHz, the ultrasonic action mode is 20s at intervals of 15s, and the ultrasonic time is 2 to 5 minutes;

[0033] 2) Electrochemically activate the carbon fiber electrode in step 1) in 0.5M sulfuric acid solution, that is, firstly apply +2.0V potential polarization on the electrode for 30-50s, and then apply -1.0V potential polarization for 10-30s. The obtained electrodes are then subjected to a 0.05-0.2V s -1 The scan speed is cyclically scanned within the potential range of 0.0-1.0V until a stable cyclic voltammogram is obtained. The schematic diagram...

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Abstract

The invention discloses a special electrode for in-vivo voltammetry analysis and a preparation method thereof. The preparation method of the special electrode comprises the following steps: 1) preparing carbon fiber coated with a carbon nanomaterial on surface; 2) preparing a carbon fiber microelectrode; 3) soaking the carbon fiber electrode prepared by the step 2) in solution for ultrasonic washing; and 4) subjecting the carbon fiber electrode prepared by the step 3) to electrochemical pretreatment to obtain the special electrode. Meanwhile, an electrochemical detection device for in vitro correction or in vivo real-time physiological activator monitoring is prepared by using the special electrode. The electrochemical detection device comprises the special electrode for physiological activator monitoring of the invention, a reference electrode and an auxiliary electrode. The electrochemical detection device prepared by using the special electrode of the invention can meet the requirements for the in-vivo voltammetry analysis of physiological activators and has a promising application prospect in researches in fields of neurophysiology, clinical medicine and the like.

Description

technical field [0001] The invention relates to a special electrode capable of satisfying living body voltammetry analysis of physiologically active substances and a preparation method thereof. Background technique [0002] In recent years, the research on the change rules of chemical substances in physiological and pathological processes has become a frontier issue of widespread concern, mainly because the real-time changes of chemical substances in vivo can directly reflect the chemical nature of physiological and pathological processes. For example, the changes of glucose and lactic acid in the brain can directly reflect the energy metabolism process in the brain; the level of neurotransmitter dopamine in the brain and its changing rules are directly related to important physiological phenomena such as body movement and mental activity; the existence of ascorbic acid in the brain The level is closely related to the oxidative damage of free radicals in the body and the syn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
Inventor 毛兰群向玲戴黎明
Owner INST OF CHEM CHINESE ACAD OF SCI
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