Method for preparing biological preparation for efficiently poisoning plant nematodes

A technology of biological preparations and plants, applied in the direction of botany equipment and methods, biocides, nematicides, etc., to achieve significant poisoning effect, simple method, and environment-friendly effect

Inactive Publication Date: 2009-11-25
NANJING FORESTRY UNIV
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  • Description
  • Claims
  • Application Information

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  • Method for preparing biological preparation for efficiently poisoning plant nematodes
  • Method for preparing biological preparation for efficiently poisoning plant nematodes

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[0016] Example 1

[0017] 1. Preparation of sterilizing biologics

[0018] Bacillus amyloliquefaciens was inoculated on beef extract peptone slant medium and cultured at 25~37℃ for 24 hours to obtain test tube species, which were made into 10 9 cfu / mL seed liquid, inoculate into 250mL Erlenmeyer flask liquid medium, the liquid medium formula is: 2-10% beef extract, 1-10% peptone powder, 0.1-3% K 2 HPO 4 , 0.1~1% MgSO 4 , 1~5% NaCl, 100mL of fresh liquid culture medium per bottle, 1mL of inoculation solution, shake culture at pH 6.5~8, 25~37℃, shake culture for 2-15 days, rotate at 120rpm, ferment the culture Centrifuge at 4000rpm for 20min, and the supernatant is a biological preparation with high toxic nematicidal activity. The control is the supernatant of the sterile liquid medium centrifuged at 4000 rpm.

[0019] 2. Cultivation and preparation of test nematodes

[0020] First inoculate Botrytis cinerca on the PDA plate, culture it at 25°C and without light until the hyphae of ...

Example Embodiment

[0026] Example 2

[0027] 1. Preparation of biocidal preparation: same as part "1" in Example 1

[0028] 2. Preparation of test nematodes: Radopholus similis and Aphelenchoides besseyi were separated from the host plant by the funnel method, and the preparation of the remaining nematodes was the same as that of the "2" part in Example 1.

[0029] 3. Method for determining drug efficacy: same as part "3" in Example 1

[0030] 4. Test results

[0031] The results (Table 2) show that the biological preparation has nematicidal activity against various nematodes listed in Table 2, and against banana perforation nematodes, rice stem nematodes, pine wood nematodes, pine wood nematodes, and large nucleus smooth blade nematodes. The killing rate of nematodes in 24 hours reached 100%, and the killing rate of the control was 0%, indicating that it has high killing activity and broad killing spectrum, and has good application development prospects.

[0032] Table 2 Comparison of nematicidal a...

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Abstract

The invention relates to a method for preparing a biological preparation for efficiently poisoning plant nematodes, which belongs to the technical field of microbial pesticides. A strain that the invention relates to is Bacillus amyloliquefaciens. The method for preparing the biological preparation comprises the following steps that: a Bacillus amyloliquefaciens test tube seedling is inoculated to a liquid culture medium (the formula is 2 to 10 percent of beef extract, 1 to 10 percent of peptone powder, 0.1 to 3 percent of K2HPO4, 0.1 to 1 percent of MgSO4, and 1 to 5 percent of NaCl); and the volume ratio of loading capacity is 2/5, a shaking table performs shaking culture under the conditions that the pH value is between 6.5 and 8 and the temperature is between 25 and 37 DEG C, the rotating speed is 120 rpm, cultured zymotic fluid is centrifugated for 20 min (4,000 rpm) after 2 to 15 days, and a supernatant fluid is the biological preparation with high nematicidal activity. Through the determination of pesticide effectiveness, the product has 100 percent of poisoning rate on the plant nematodes such as Bursaphelenchusxylophilus, Radopholus similis, Aphelenchoides besseyi and the like, and has broad development and application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and specifically refers to a method for preparing a highly effective biological agent for poisoning and killing plant parasitic nematodes by using Bacillus amyloliquefaciens. Background technique [0002] Plant-parasitic nematodes refer to nematodes that parasitize plants and have a unique feeding organ—a stylet. There are more than 200 genera and more than 5,000 species that have been recorded so far. Almost all plants are parasitized and damaged by nematodes. According to the statistics of the Food and Agriculture Organization of the United Nations, the annual losses caused by nematodes in the world exceed 100 billion US dollars. In my country, it is reported that there are nearly a hundred kinds of nematode diseases that seriously threaten agricultural, forestry, economic and other crops. For example, pine wood nematode is a devastating forest disease that mainly harms Pinus pla...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P5/00
Inventor 吴小芹朱丽梅叶建仁徐旭凌陈江天
Owner NANJING FORESTRY UNIV
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