Method for producing Buthus martensii Karsch toxin AGAP

A production method, the SUMO-AGAP technology, is applied in the field of medicine and biology to achieve the effects of increasing yield, saving costs, and easy operation
CN101591668BInactive Publication Date: 2011-01-26JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
Publication Date
2011-01-26
Estimated Expiration
Not applicable Β· inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention belongs to the field of medical biology, and particularly relates to a production method for recombining scorpion toxin protein AGAP. The method comprises the following steps: firstly, obtaining SUMO-AGAP fusion genes with His6 labels by using overlapping PCR technology; secondly, constructing a recombinant expression vector containing the SUMO-AGAP fusion genes; thirdly, transferring the constructed expression vector to a host cell to express; and finally, cracking the cell by using a physical method or a chemical agent, extracting recombinant fusion protein and His purified fusion protein to obtain pure SUMO-AGAP fusion protein, carrying out enzyme cutting reaction, releasing AGAP, and further purifying the fusion protein to obtain the purified AGAP protein. The method greatly improves the yield, saves the cost, and is simple and convenient to operate; and the AGAP produced by the method has broad biologic activity, can restrain the proliferation of various tumor cells, and simultaneously has analgesia effect.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of medical biotechnology, in particular, relates to a method for preparing a large amount of recombinant scorpion venom protein AGAP with high biological activity in prokaryotic cells, especially E. Biological activity, can inhibit the proliferation of a variety of tumor cells, and has an analgesic effect. Background technique

[0002] In traditional Chinese medicine, scorpions are an important source of medicine. In the medical history of our country, scorpion was used as medicine very early. With the development of science and technology, studies have found that scorpion venom is the main active substance of the traditional medicinal material scorpion, which exists in the poison sac of the tail of the scorpion and is discharged from the needle when stabbed. Scorpion venom is a biological toxin second only to snake venom in toxicity. A complex mixture of ingredients. Its main active ingredients are toxic proteins...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More