Kit for detecting human BDNFmet gene variation

Abstract

The invention relates to a kit used for accurately detecting whether valine at the 66th position of BDNF in a specimen is variated into methionine by a fluorescence PCR detection technique. The invention provides effective guide for clinically and individually treating mental sickness, such as anxiety neurosis, melancholia, post-traumatic stress syndrome, and the like correlative to BDNF gene variation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and is a kit for accurately detecting whether valine at position 66 of brain-derived neurotrophic factor (BDNF) in a sample is mutated into methionine through fluorescent PCR detection technology. Background technique [0002] Traditional detection techniques for detecting whether the valine at position 66 of brain-derived neurotrophic factor (BDNF) in a specimen is mutated into methionine include: PCR-restriction enzyme digestion technology and PCR-sequencing technology. Both of these methods require subsequent testing after PCR testing. Restriction enzyme digestion methods often have incomplete digestion, which affects the accuracy of detection; and sequencing technology is expensive and not suitable for large-scale screening. The present invention does not need follow-up detection after PCR, is quick and easy; detects with a probe, and the result is accurate; the cost is low, and it is especially...

AI Technical Summary

Problems solved by technology

Restriction enzyme digestion methods often have incomplete digestion, which affects the accuracy of detection; and sequencing technology is expensive and not suitable for large sample screening

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