Unlock instant, AI-driven research and patent intelligence for your innovation.

Primer pair for identifying booklice in stored booklices and application thereof

A storage booklice and primer pair technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of difficult to distinguish similar species and non-adult identification, and achieve fast and accurate The effect of identification

Inactive Publication Date: 2009-12-23
CHINA AGRI UNIV
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] For a long time, the traditional insect species identification technology is mostly based on the morphological characteristics of adults, which cannot realize the identification of non-adult states such as eggs, larvae and pupae, and it is difficult to distinguish similar species with subtle differences.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer pair for identifying booklice in stored booklices and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1. Identification of book louse from book louse in storage by PCR using specific primers

[0015] (1) Extraction of book lice genomic DNA

[0016] The genomic DNA of single-headed book lice from different geographic populations was extracted by CTAB method, including book louse collected from the Czech Republic and the United States, book louse collected from China, the Czech Republic, and the United States, and the book louse collected from China and the Czech Republic. Lice, book lice collected from China, Czech Republic, and the United States, book louse, colorless book louse collected from China, Czech Republic, and the United States, book louse collected from Czech Republic, and the United States, and false book louse collected from China. The above species of book lice were confirmed by morphological characteristics.

[0017] After killing the single head of the book lice to be checked with absolute ethanol, wash it with double distilled water and dry it ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a primer pair for identifying booklice in stored booklices and an application thereof. The nucleotide sequences of the primer pair for identifying booklice in stored booklices are respectively a sequence 1 and a sequence 2 in a sequence table. The invention also discloses a method for using the primer pair to identify the booklice in stored booklices. The method takes genome group DNA of the stored booklices to be measured as a template, uses the primer pair to carry out PCR amplification, and detects the PCR amplification product, and if the size of the PCR amplification product is 250 to 500bp, the stored booklice to be measured is the booklice. By the method for identifying booklice in stored booklices, the invention realizes fast and accurate identification of the booklice, distinguishes the booklice from other common stored booklices in the world, and has the characteristics of time saving, high efficiency and intuition and the like. The method can be widely used in inspection quarantine departments and grain storage departments.

Description

technical field [0001] The present invention relates to a primer pair for identifying book louse from book louse in storage and its application. Background technique [0002] For a long time, traditional insect species identification techniques are mostly based on the morphological characteristics of adults, which cannot identify non-adult states such as eggs, larvae and pupae, and it is difficult to distinguish similar species with subtle differences. Since the 1990s, with the development of modern molecular biology technology, many scholars at home and abroad have carried out molecular identification of various insects. When using modern molecular biology for insect identification research, the information of gene sequence represents the essence of heredity, which is not affected by the individual development status and environmental conditions of the specimen, and can provide sufficient and quantitatively measurable genetic information. Therefore, it is an efficient and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
Inventor 李志红赵朔
Owner CHINA AGRI UNIV