Human Bcl-2 and human VEGF165 double-gene co-expression recombinant vector and building method thereof
A recombinant vector, bcl-2 technology, applied in botany equipment and methods, biochemical equipment and methods, genetic engineering, etc., can solve unsatisfactory problems, achieve faster recovery, avoid gene expression inhibition, and promote angiogenesis Effect
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[0042] Figure 1-11 Constitute the specific embodiment 1 of the present invention.
[0043] Human Bcl-2 and Human VEGF 165 Double-gene co-expression recombinant vector, including human Bcl-2 gene and human VEGF 165 Gene; wherein, the human Bcl-2 gene sequence is described in the sequence table SEQ ID No. 1; human VEGF 165 The gene sequence is as described in SEQ ID No. 2 in the sequence table; the vector is an adenovirus vector, and other vectors such as other adeno-associated viruses, lentiviruses and plasmids can also be used.
[0044] The human Bcl-2 and human VEGF 165 The construction process of the double gene co-expression recombinant adenoviral vector is as follows: figure 1 As shown, its construction method is as follows:
[0045] 1. Main experimental reagents and equipment:
[0046] Biochemical reagents: DNA Marker (Takara, Japan), tryptone, yeast extract, agar powder (Oxoid, Japan), agarose (Biowest, Spain), kanamycin, ampicillin (Amresco, USA) and Trizol reag...
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