Shaanxi dulcite A extracted from Shaanxi japonicas and extracting process thereof

An extraction process, dulcitol technology, applied in Shaanxi dulcitol A extracted from Shaanxi Euonymus and its extraction process field, can solve the problems such as no public reports of active ingredients, and achieve convenient operation, economical and social The effect of huge benefits and advanced preparation methods

Inactive Publication Date: 2010-03-10
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Why does Euonymus shaanxi have insecticidal activity? Th...

Method used

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  • Shaanxi dulcite A extracted from Shaanxi japonicas and extracting process thereof
  • Shaanxi dulcite A extracted from Shaanxi japonicas and extracting process thereof
  • Shaanxi dulcite A extracted from Shaanxi japonicas and extracting process thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Take 10kg of fresh Euonymus shaanxi stems and leaves, and use a flash extractor to extract twice in 70% acetone aqueous solution at room temperature at 18°C. The first time is 20 minutes, the second time is 15 minutes, and the extracts are combined at 45°C. Recover the solvent under reduced pressure to obtain 410 g of dry powder, add water to disperse and dissolve the dry powder, centrifuge, and use the supernatant macroporous adsorption resin column Diaion HP-20, rinse with water until the eluent is colorless, and then rinse with 10% methanol until the eluent is colorless , the obtained 10% methanol eluent, the solvent was recovered under reduced pressure at 45° C. to obtain 15 g of dry powder, which was dissolved in water and applied to a gel Toyopearl HW-40 column, and eluted with water to obtain an eluent, which was dripped on a thin film Expand on the laminate, spray with anisaldehyde-concentrated sulfuric acid color developer after development, at 105 ° C, the frac...

Embodiment 2

[0023] Take 15 kg of fresh stems and leaves of Euonymus shaanxi, and use a flash extractor to extract twice in 70% acetone aqueous solution at room temperature at 20°C, each time for 18 minutes. After the extracts are combined, the solvent is recovered under reduced pressure at 45°C to obtain Dry powder 605g, add water to disperse and dissolve the dry powder, centrifuge, put the supernatant on a macroporous adsorption resin column Diaion HP-20, wash with water until the eluent is colorless, then wash with 10% methanol until the eluent is colorless, the obtained 10% The methanol eluent was recovered under reduced pressure at 45°C to obtain 23 g of dry powder. The dry powder was dissolved in water and applied to a gel Toyopearl HW-40 column, and eluted with water to obtain an eluent. After spraying with anisaldehyde-concentrated sulfuric acid developer, at 105°C, the fraction with an RF value of 0.4 will first appear blue and then turn red. Collect this fraction to obtain compone...

Embodiment 3

[0025] Take 20kg of fresh Euonymus shaanxi stems and leaves, and use a flash extractor to extract twice in 70% acetone aqueous solution at room temperature at 22°C. The first time is 15 minutes, the second time is 20 minutes, and the extracts are combined at 45°C. Recover the solvent under reduced pressure to obtain 825 g of dry powder, add water to disperse and dissolve the dry powder, centrifuge, put Diaion HP-20 on the macroporous adsorption resin on the supernatant, wash with water until the eluent is colorless, and then rinse with 10% methanol until the eluent is free color, the obtained 10% methanol eluent was recovered under reduced pressure at 45°C to obtain 32g of dry powder, and the dry powder was dissolved in water and applied to a gel Toyopearl HW-40 column, and eluted with water to obtain an eluent, which was dripped on Developed on a thin-layer plate, sprayed with anisaldehyde-concentrated sulfuric acid color developer after development, at 105 ° C, the fraction w...

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Abstract

The invention relates to a Shaanxi dulcite A extracted from Shaanxi japonicas and an extracting process thereof. The process comprises the steps as follows: taking stems and leaves of fresh Shaanxi japonicas; smashing tissues of the stems and the leaves in aqueous acetone at room temperature, extracting, decompressing and recovering a solvent to obtain dry powder; adding water to dissolve the drypowder; centrifuging, absorbing resin columns in large holes on supernate, flushing with water till spent regenerant is colorless; then flushing with 10 % methyl alcohol till the spent regenerant is colorless so as to obtain 10 % methyl alcohol spent regenerant; decompressing and recovering a solvent at low temperature to obtain dry powder; dissolving the dry powder to coat a gel column, eluting with water to obtain spent regenerant; dropping the spent regenerant on a thin layer plate for unfolding, then atomizing with anisaldehyde-concentrated sulfuric acid color development reagent, developing the color of the fraction to be blue at the first and then the changing the color into red, and respectively enabling the fraction to be subjected to a gel Toyopearl HW-40 column, an MCI Gel, CHP-20 column, a Sephadex LH-20 column and a silicagel column to obtain the colorless oleosus Shaanxi dulcite A. The Shaanxi dulcite A is a new compound which has convenient operation of preparation method, strong reliability and huge economic and social benefits.

Description

1. Technical field [0001] The invention relates to Shaanxi dulcitol A extracted from Shaanxi Euonymus and its extraction process. 2. Background technology [0002] Shaanxi euonymus (Euonymus schensianus Maxim.) is a plant of the genus Euonymus in the family Celastraceae, also known as golden silk hanging butterfly and golden butterfly. According to the "Xinhua Compendium of Materia Medica", most of the plants in this family have insecticidal activity, mainly concentrated in the genus Tripterygium genus, Euonymus genus, and Snakevine genus. Since the 1980s, Chinese scholars have conducted extensive research on the insecticidal activity of many plants in the family Euonymus, and found that Euonymus shaanxi has antifeedant and anesthetic effects on insect larvae. Widely distributed in the southern part of North China and the provinces of central China, mainly in the Funiu Mountains of Henan Province. Why does Euonymus shaanxi have insecticidal activity? There is no public re...

Claims

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Application Information

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IPC IPC(8): C07D307/12
Inventor 郑晓珂冯卫生郭锦辉王小兰
Owner HENAN UNIV OF CHINESE MEDICINE
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