Method for extracting and separating ginkgo leaf polyprenol acetic ester from ginkgo leaf by supercritical carbon oxide

Abstract

The invention discloses a method for extracting and separating ginkgo leaf polyprenol acetic ester from a ginkgo leaf by supercritical carbon oxide. The first extraction comprises the following steps of: putting dried and pulverized ginkgo leaf powder into an extraction kettle, introducing the supercritical carbon oxide with the pressure of 25-35 MPa into the extraction kettle; controlling the temperature of the extraction kettle to 40-70 DEG C; circularly extracting the ginkgo leaf for 5-10 hours; and releasing an ointment containing the ginkgo leaf polyprenol acetic ester from a separation kettle in two-stage series connection. The second extraction comprises the following steps of: filling the ointment which is obtained in the first extraction into the extraction kettle; introducing the supercritical carbon oxide with the pressure of 5-15MPa into the extraction kettle; controlling the temperature of the extraction kettle to 20-30 DEG C; circularly extracting the ginkgo leaf for 3-8hours; and releasing an oily matter of the ginkgo leaf polyprenol acetic ester from the extraction kettle. The method has low cost and high content without organic solvent extraction, and the extractwith high purity can be used as an important material applied to healthcare food, cosmetics, drugs and the like.

Description

Technical field

[0001] The invention relates to a method for extracting and separating ginkgo biloba polypentenol acetate twice from ginkgo biloba using supercritical carbon dioxide. Background technique

[0002] Ginkgo is called "living fossil" and is one of the ancient tree species unique to our country. Ginkgo biloba leaves are rich in active ingredients such as polypentenol esters, flavonoids, and terpene lactones. These substances have strong pharmacological effects on aplastic anemia, various liver diseases, diabetes, and central system diseases.

[0003] The polypentenol acetate in Ginkgo biloba leaves belongs to the betula polypentenol acetate, which is mainly composed of 14-20 isopentenyl units, with 2 trans-isopentenyl and 11-17 The long-chain compound composed of cis-prenyl and α-acetoxy accounts for about 80% of the total content of polypentenols in Ginkgo biloba leaves. Polypentenol acetate can be hydrolyzed into polypentenol, and polypentenol can be converted into p...

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