Method for extracting and separating ginkgo leaf polyprenol acetic ester from ginkgo leaf by supercritical carbon oxide

A technology of leaf polyprenyl acetate and carbon dioxide, which is applied in the field of double extraction and separation of ginkgo biloba polyprenyl acetate, can solve the problems of difficult industrial production, low product yield, and large consumption. Achieve the effects of shortening the production cycle, high content and purity, and improving production efficiency

Inactive Publication Date: 2012-10-10
江苏同源堂生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two kinds of processes have all used solvent extraction when preparing polyprenol acetate, and there are following disadvantages: (1) the residue of solvent may bring toxic and side effects; The ginkgolic acid will be extracted together with active substances effective to the human body, resulting in a decline in product quality; (3) the extraction process uses a large number of low-boiling point solvents such as petroleum ether and n-hexane, which consumes a lot at one time and is not easy to recycle, causing environmental pollution; (4) The product yield is low, the cost is high, and it is difficult to realize industrialized production

Method used

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  • Method for extracting and separating ginkgo leaf polyprenol acetic ester from ginkgo leaf by supercritical carbon oxide

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Effect test

Embodiment 1

[0017] Using supercritical carbon dioxide to extract and separate ginkgo biloba polypentenol acetate from ginkgo biloba, firstly, 10 kilograms of ginkgo biloba powder with a crushed particle size of 60 mesh is charged into the extraction kettle, and the carbon dioxide high-pressure pump is started to increase the pressure to 30 MPa, the supercritical carbon dioxide is sent into the extraction kettle, the temperature of the extraction kettle is 65℃, the carbon dioxide fluid after passing through the extraction kettle enters the first and second two separation kettles in series, the temperature of the first stage separation kettle is 40 ℃, the pressure is 5 MPa, the temperature of the second-stage separation kettle is 30 ℃, the pressure is 3 MPa, and the carbon dioxide fluid is refrigerated and compressed back to the gas storage tank. The carbon dioxide flow rate is 0.3-1.2 cubic meters per hour. After 6 hours of extraction, stop the pump and depressurize, release the ginkgo bilob...

Embodiment 2

[0020] First, put 20 kg of ginkgo leaf powder with a crushed particle size of 80 mesh into the extraction kettle, start the carbon dioxide high-pressure pump, increase the pressure to 25 MPa, and send the supercritical carbon dioxide into the extraction kettle. The temperature of the extraction kettle is 60 ℃. The carbon dioxide fluid after the kettle enters the first-stage and second-stage two separation kettles in series. The temperature of the first-stage separation kettle is 30°C and the pressure is 5 MPa, and the temperature of the second-stage separation kettle is 30°C and the pressure is 4 MPa. The carbon dioxide fluid is refrigerated and compressed back to the gas storage tank. The carbon dioxide flow rate is 0.3-1.2 cubic meters per hour. After 10 hours of circulating extraction, the pump is stopped and the pressure is reduced, and the ginkgo leaf extract residue is released from the extraction kettle. Discharge about 1700 grams of ginkgo biloba polypentenol acetate oin...

Embodiment 3

[0023] Firstly, 15 kg of Ginkgo biloba powder with a crushed particle size of 100 mesh is put into the extraction kettle, the high pressure carbon dioxide pump is started, and the pressure is increased to 30 MPa, and the supercritical carbon dioxide is fed into the extraction kettle. The temperature of the extraction kettle is 55℃. The carbon dioxide fluid after the kettle enters the first and second two separation kettles in series. The temperature of the first-stage separation kettle is 35°C and the pressure is 5 MPa, and the temperature of the second-stage separation kettle is 30°C and the pressure is 4 megapascals. The carbon dioxide fluid is refrigerated and compressed back to the gas storage tank. The carbon dioxide flow rate is 0.3-1.2 cubic meters per hour. After 8 hours of circulating extraction, the pump is stopped and the pressure is reduced, and the ginkgo leaf extract is released from the extraction kettle. Discharge about 1360 grams of ginkgo biloba polypentenol ac...

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Abstract

The invention discloses a method for extracting and separating ginkgo leaf polyprenol acetic ester from a ginkgo leaf by supercritical carbon oxide. The first extraction comprises the following steps of: putting dried and pulverized ginkgo leaf powder into an extraction kettle, introducing the supercritical carbon oxide with the pressure of 25-35 MPa into the extraction kettle; controlling the temperature of the extraction kettle to 40-70 DEG C; circularly extracting the ginkgo leaf for 5-10 hours; and releasing an ointment containing the ginkgo leaf polyprenol acetic ester from a separation kettle in two-stage series connection. The second extraction comprises the following steps of: filling the ointment which is obtained in the first extraction into the extraction kettle; introducing the supercritical carbon oxide with the pressure of 5-15MPa into the extraction kettle; controlling the temperature of the extraction kettle to 20-30 DEG C; circularly extracting the ginkgo leaf for 3-8hours; and releasing an oily matter of the ginkgo leaf polyprenol acetic ester from the extraction kettle. The method has low cost and high content without organic solvent extraction, and the extractwith high purity can be used as an important material applied to healthcare food, cosmetics, drugs and the like.

Description

Technical field [0001] The invention relates to a method for extracting and separating ginkgo biloba polypentenol acetate twice from ginkgo biloba using supercritical carbon dioxide. Background technique [0002] Ginkgo is called "living fossil" and is one of the ancient tree species unique to our country. Ginkgo biloba leaves are rich in active ingredients such as polypentenol esters, flavonoids, and terpene lactones. These substances have strong pharmacological effects on aplastic anemia, various liver diseases, diabetes, and central system diseases. [0003] The polypentenol acetate in Ginkgo biloba leaves belongs to the betula polypentenol acetate, which is mainly composed of 14-20 isopentenyl units, with 2 trans-isopentenyl and 11-17 The long-chain compound composed of cis-prenyl and α-acetoxy accounts for about 80% of the total content of polypentenols in Ginkgo biloba leaves. Polypentenol acetate can be hydrolyzed into polypentenol, and polypentenol can be converted into p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/145C07C67/48
CPCY02P20/54
Inventor 刘春卉俞建国杨秀伟马维新
Owner 江苏同源堂生物工程有限公司
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