Method for determining dopamine by utilizing charge recognition effect

Abstract

The invention discloses a method for determining dopamine by utilizing charge recognition effect, which comprises the steps of: polishing a gold electrode by using alumina powder, orderly soaking and washing the gold electrode in nitric acid, anhydrous ethanol and pure water, taking the gold electrode out, and then performing ultrasonic washing on the gold electrode for 5 minutes to obtain a clean bare gold electrode; placing the bare gold electrode into 10 to 15mL of 0.1mol / L HCl solution containing 0.01mol / L cysteine for 6h to obtain a cysteine modified electrode; and then soaking the electrode in 10 to 15mL of 0.05mol / L mixed solution of diethylenetriaminepentaacetic acid which contains 0.02mol / L 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and 0.04mol / L N-hydroxysuccinimide for 5 hours to obtain a diethylenetriaminepentaacetic acid / cysteine / gold modified electrode. The method is used for detecting the content of DA in human serum samples; and common interference substances such as ascorbic acid and uric acid, and other substances with oxidation potential close to that of the DA have no obvious interference. The method can be used for measuring the concentration of the dopamine in serum, and has the advantages of simple manufacturing and convenient use.

Description

technical field [0001] The invention relates to a method for determining dopamine by using charge recognition effect, which can be used for the determination of dopamine in human serum samples. Background technique [0002] Dopamine (3,4-dihydroxy-β-phenylethylamine, DA) is a very important neurotransmitter and is closely related to Parkinson's disease, Huntington's disease and ADHD. At present, there are many methods for measuring DA concentration. Electrochemical methods are widely used because of their high sensitivity and good selectivity. Especially, electrochemical biosensors can perform in vivo analysis, which is unmatched by other methods. Bare electrodes such as diamond electrodes, glassy carbon electrodes, platinum electrodes, and gold electrodes can be used to measure DA, but ascorbic acid (AA) and uric acid (UA) interfere with the final measurement results because the redox potential is similar to DA. In order to solve this problem, many efforts have been made, ...

AI Technical Summary

Problems solved by technology

The most typical membrane in the ion exchange membrane is the Nafion membrane, but the Nafion membrane has several common disadvantages: non-uniform membrane thickness, poor reproducibility, long response time, and high price, and self-assembly can overcome these shortcomings of the Nafion membrane

There are no reports in the literature on the use of electrostatic recognition of DTPA chelates for the determination of DA

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