Method for preparing neutral polysaccharide from gynostemma pentaphylla

A technology of neutral polysaccharides and Gynostemma pentaphyllum, which is applied in the field of purification, extraction and separation of a neutral polysaccharide, and can solve the problems of lack of procedures such as decolorization

Inactive Publication Date: 2010-06-02
双惠橡胶南通有限公司 +1
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The technology used in Chinese Patent Publication No. CN 1490338A, although it has carried out the process of removing protein, lacks procedures such as decolorization, and the Gynostemma pentaphyllum polysaccharide obtained by this method is only a crude product, containing a large amount of other components such as pigments
Uniform Gynostemma pentaphyllum polysaccharide fraction has not been separated and purified yet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing neutral polysaccharide from gynostemma pentaphylla
  • Method for preparing neutral polysaccharide from gynostemma pentaphylla
  • Method for preparing neutral polysaccharide from gynostemma pentaphylla

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh 500g of dry Gynostemma pentaphylla, add 2500mL95% ethanol, stir at 50°C for 1 hour, then filter and collect the filter residue; then filter the filter residue with 95% ethanol at 50°C for 1 hour and then filter and collect the filter residue; then filter the filter residue with 95 % ethanol was refluxed and stirred at 50°C for 1 hour, and the filter residue was collected by filtration; after the filter residue was naturally air-dried, 3000mL of distilled water was added, stirred at 95°C for 10h, and the filtrate was collected by filtration, repeated 3 times; Concentrate to 1L under vacuum and reduced pressure at 50°C, dialyze in running water for 48 hours;

[0025] Add 4 times the volume of ethanol to the dialyzed solution, store at 4°C for 12h; then centrifuge at 5000rpm for 10min with a high-speed centrifuge, collect the precipitate, wash with ethanol, acetone and ether in turn, and dry in vacuum at 40°C 6h, to obtain the brown polysaccharide of Gynostemma penta...

Embodiment 2

[0032] Weigh 20 kg of dried Gynostemma pentaphyllum, add 120 L of distilled water, put it into an extraction tank, stir at 95 ° C for 10 h, filter and collect the filtrate, repeat 2 times; combine the filtrates collected each time above, and concentrate under vacuum at 50 ° C to 40L, dialyzed in running water for 48h;

[0033] Add 4 times the volume of industrial ethanol to the dialyzed solution, and store it at 4°C for 10 hours; then use a high-speed centrifuge to centrifuge at 5000rpm for 10 minutes, collect the precipitate, wash it with ethanol, acetone and ether in sequence, and vacuum at 45°C Dried for 6 hours to obtain brown polysaccharides from Gynostemma pentaphyllum;

[0034] Weigh 10g Gynostemma chinensis crude polysaccharide, add 200mL deionized water to make 5% crude polysaccharide solution, adjust the pH to 5.5 with 1mol / L HCl, add 0.5g papain, stir at 65°C for 3h, heat to 100°C, and keep for 6min , inactivate, terminate the enzyme reaction;

[0035] Add 1800mL ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates a method for preparing neutral polysaccharide from gynostemma pentaphylla, which is characterized by comprising three steps of: extracting a gynostemma pentaphylla concentrating solution by adding alcohol and drying in vacuum to obtain dark brown gynostemma pentaphylla rough polysaccharide; dissolving the gynostemma pentaphylla rough polysaccharide in water, adding papain, reacting and centrifugating and collecting supernate, dialyzing, adding alcohol to deposit polysaccharide, centrifugally collecting the polysaccharide, freezing, and drying to obtain white gynostemma pentaphylla fine polysaccharide; dissolving the gynostemma pentaphylla fine polysaccharide in water, applying an anion-exchange column, carrying out gradient elution by using distilled water and NaCl solution in sequence, applying a gel column, then carrying out gradient elution by using NaCl solution, collecting a main peak, freezing and drying after being dialyzed to obtain the gynostemma pentaphylla neutral polysaccharide GPMPA which is pyranose containing rhamnose, arabinose, seminose, glucose and galactose.

Description

technical field [0001] The invention relates to a method for preparing neutral polysaccharides from Gynostemma pentaphyllum, in particular to a method for extracting, separating and purifying a neutral polysaccharide from Gynostemma pentaphyllum, a natural resource in Qinba mountainous area. Background technique [0002] Gynostemma pentaphyllum Makino is a perennial herbaceous vine of Cucurbitaceae Gynostemma genus. It has the functions of enhancing immunity, anti-tumor, lowering blood pressure, lowering blood fat, and anti-oxidation, and is safe and non-toxic. At present, there are 13 species of Gynostemma pentaphyllum plants known in the world, and 11 species are in my country, of which 7 species are unique to my country, accounting for 53.8% of the total number of species. Gynostemma is mainly distributed in some Asian countries. In my country, Shaanxi Gynostemma is the most abundant resource. It is mainly distributed in 37 counties and cities centered on Qinba Mountains....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 尚晓娅钦传光徐春兰牛卫宁钞愈
Owner 双惠橡胶南通有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap