Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method and kit for identifying early embryonic sex of pig by dual temperature multiplex PCR

A kit and multiple technologies, applied in the field of livestock sex control and identification, can solve the problems of unseen pig early embryo sex identification methods, and achieve the effects of saving breeding costs, improving efficiency, and saving samples

Inactive Publication Date: 2013-04-03
JILIN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the sex identification methods of livestock mainly include cytogenetic method, H-Y antigen method, sex-specific DNA probe method, PCR method, LAMP method, etc., which are mainly used for sex identification of cattle, sheep or other economic animals. There are literature reports on the method of sex identification of early pig embryos

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and kit for identifying early embryonic sex of pig by dual temperature multiplex PCR

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. Sample handling:

[0046] A: If the sample to be tested is pig embryo or cell:

[0047] Separate 4 or more cells from the early pig embryos to be tested, put them into a 0.2ml PCR tube, add 10ul of cell lysate, place in a PCR instrument at 56°C for 10min, 94°C for 5min; centrifuge at 12000rpm for 2min, and take the supernatant , stored at -20°C for later use;

[0048] B: If the sample to be tested is pig early fetus or pig tissue of unknown sex:

[0049] Take the 1mm×1mm size tissue block of the sample to be tested, put it into a 0.2ml PCR tube, add 50ul of tissue lysate, place it in a PCR instrument at 56°C for 50min, and at 94°C for 5min; centrifuge at 12000rpm for 2min, take the supernatant and store it at -20°C for later use ;

[0050] 2. Primer design:

[0051] A pair of dual-temperature PCR amplification primers were designed according to the porcine SRY gene sequence (Genbank accession number: U49860.2). In addition, a pair of dual-temperature PCR amplifi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method and a kit for identifying the early embryonic sex of a pig by dual temperature multiplex PCR. A dual temperature multiplex PCR reaction system with rapidness, sensitiveness and high accuracy, which is suitable for identifying the early embryonic sex of the pig, is used for identifying the early embryonic sex of the pig. The kit which is developed by the dual temperature multiplex PCR reaction system and used for identifying the early embryonic sex of the pig fully plays the characteristics of high efficiency, economy, simpleness, convenience and high reaction specificity of the dual temperature multiplex PCR and realizes the rapid identification of the early embryo of the pig, thereby realizing the sex control of the pig, improving the economic benefits ofpig breeding industry, saving the breeding cost and providing convenience.

Description

technical field [0001] The invention relates to a PCR reaction technology system suitable for sex identification of early pig embryos, and further discloses a double-temperature multiplex PCR method for sex identification of early pig embryos, and also provides corresponding kits, belonging to livestock sex control Identification of technical fields. Background technique [0002] The sex control of domestic animals refers to the technology of making female breeding animals reproduce the desired sex offspring according to people's wishes through human intervention, which is of great significance in animal husbandry production. [0003] At present, the sex identification methods of domestic animals mainly include cytogenetic method, H-Y antigen method, sex-specific DNA probe method, PCR method, LAMP method, etc., which are mainly used for sex identification of cattle, sheep or other economic animals. There are reports in the literature on methods for sex identification of ear...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 欧阳红生李小平逄大欣高飞闫森赖良学王铁东李莉于浩
Owner JILIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com