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Casein nanoparticle

A casein and particle technology, applied in the field of casein nanoparticles, can solve the problems of anionic compound binding, unable to maintain, unable to maintain the state and so on

Active Publication Date: 2010-06-23
FUJIFILM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, if the pH is lowered to a pH below the isoelectric point of casein, although it is an amphipathic protein, precipitation and aggregation occurs
Therefore, the state of this casein-poor micelle cannot be maintained, and positively charged nanoparticles cannot be prepared, and as a result, anionic compounds cannot be bound to the nanoparticles or cannot be maintained on the particle surface (Journal of the Agricultural Chemical Society of Japan chemical society of Japan), Vol. 16, No. 9, 1087-1092 (1987), Journal of the Agricultural Chemical Society of Japan, Vol. 49, No. 8, 417-424 (1975) , Journal of Dairy Research, 53, 547-555 (1986) and International Publication WO2007 / 114262 (Fuji Film)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] 100 mg of casein (derived from milk, manufactured by Wako Pure Chemical Industries, Ltd.; isoelectric point: 4.3) was mixed into 10 ml of a 50 mM citric acid solution (pH 1.9). NaOH was added to the mixed solution so that the pH could be stably maintained at pH 3. The average particle diameter of the above particles was measured using a light scattering photometer (Nano-ZS; manufactured by Malvem Instruments Ltd.). As a result, it was measured to be 22 nm, and the zeta potential was measured to be 16 mV (Table 1). In addition, it was confirmed that the particles were stably dispersed at 4°C for 10 days.

Embodiment 2 and 3

[0097] Casein nanoparticles were prepared in the same manner as in Example 1, except that the final pH after adding NaOH was set at pH 2.1 (Example 2) or pH 3.9 (Example 3). Then, particle size (nm) and zeta potential (mV) were measured. The results are shown in Table 1.

Embodiment 4

[0105] Casein nanoparticles were prepared using malic acid instead of citric acid in Example 1, and a dispersion solution with pH 2.4 was obtained. The average particle diameter of the above particles was measured using a light scattering photometer (Nano-ZS; manufactured by Malvem Instruments Ltd.). As a result, it was measured to be 38 nm, and the zeta potential was measured to be 24 mV. In addition, it was confirmed that the particles were stably dispersed at 4°C for 10 days.

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Abstract

The object of the present invention is to provide an electropositive nanoparticle and preparation method thereof. The electropositive nanoparticle can be produced without using surfactants or synthetic polymers, the size of which can be controlled, which is stable at acidic condition, and which contains an active substance therein. The present invention provides a casein particle, wherein zeta potential is positive.

Description

technical field [0001] The present invention relates to a nanoparticle for use in fields such as life sciences or medical diagnostics. More specifically, the present invention relates to a casein nanoparticle. Background technique [0002] Fine-grained materials have been expected to be widely used in biotechnology. In particular, research on the application of nanoparticle materials developed by the advancement of nanotechnology to biotechnology or medical treatment has been actively conducted in recent years. Many research results have been reported. [0003] Nanoparticles were strongly anticipated early in the field of drug delivery systems (DDS) and are extremely promising as carriers of drugs or genes. In particular, research using polymer micelles has been actively conducted. In most cases, AB- or ABA-type block copolymers are used due to their structural simplicity. Polymeric micelles are characterized by their large drug capacity, high water solubility, high str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K8/64A23L1/29A23L33/00A23L33/15
CPCA61K8/02A23V2002/00A61Q19/00A23J3/10A61K8/64B82Y5/00A61K38/00A61K2800/413A23L1/3056C07K14/4732A23L33/19Y10T428/2982A61P11/00A61P17/00A61P25/22A61P25/24A61P25/28A61P29/00A61P9/00A61P9/06A23V2200/25
Inventor 金泽克彦
Owner FUJIFILM CORP