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Preparation method of oryzenin antioxidant peptide

A technology of antioxidant peptide and rice protein, which is applied in the field of preparation of rice protein antioxidant peptide, can solve the problems of complex process route, high production cost, expensive raw materials, etc., and achieve good water solubility, high food safety, and yellowish color Effect

Inactive Publication Date: 2010-06-23
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented process involves preparation of anti- oxidative pepsin polypeptides made up mostly of rice proteins containing at least 6.6% dry weight based on their total amount of carbohydrates obtained during mill processing. These peaks have excellent properties such as strong hydrophilicity but no absorption ability towards polar molecules like calcium ion. They also show promise as potential drugs due to their unique structure and function.

Problems solved by technology

This patented technical solution describes how it was discovered during studying different types of oils used on fruits like apples or bananas. It suggests finding new ways to use them instead of traditional ones without causing damage due to excessive exposure time. Additionally, there were limitations when trying to make certain kinds of molecules better than others because they could cause harm if absorbed too much through their skin barrier.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0017] (1) Pass the raw rice dregs through a 60-mesh sieve, weigh 100g of the sieved rice dregs, add 1kg of drinking tap water, stir well, and soak for 12 minutes; (2) Pour the rice dregs liquid into a colloid mill and grind 2min; (3) Adjust the pH of the milled homogenized feed solution to 9.0, add 15ml of phosphate buffer, keep the pH value constant, add alkaline protease for hydrolysis, the amount of enzyme added is 300U / g, and the hydrolysis time is 2 hours. The temperature is 50°C, and the degree of hydrolysis is controlled to be 12wt%; (4) The hydrolyzate is deactivated at 85°C, and after the hydrolyzate is cooled to room temperature, it is centrifuged at a speed of 3000r / min, and the supernatant is taken; (5) The above The supernatant liquid is placed in a vacuum concentration device, and vacuum concentrated at a vacuum degree of 0.09Mpa and a temperature of 45°C. When the solid content concentration reaches 50wt%, it is pre-frozen; the freeze-drying temperature is -30°C...

Embodiment 2

[0027] (1) Pass the raw rice dregs through a 80-mesh sieve, weigh 100g of sieved rice dregs, add 800g of drinking tap water, stir well, and soak for 8 minutes; (2) Pour the rice dregs liquid into a colloid mill and grind 3min; (3) Adjust the pH of the milled homogenized feed solution to 8.5, add 10ml of phosphate buffer, keep the pH value constant, add alkaline protease for hydrolysis, the amount of enzyme added is 300U / g, and the hydrolysis time is 2 hours. The temperature is 50°C, and the degree of hydrolysis is controlled to be 15wt%; (4) The hydrolyzate is inactivated at 85°C, and the hydrolyzate is cooled to room temperature, and the supernatant is taken by centrifugation at a speed of 3000r / min; (5) The supernatant liquid is placed in a vacuum concentration device, and vacuum concentration is carried out at a vacuum degree of 0.09Mpa and a temperature of 45°C. When the solid content concentration is 50wt%, it is pre-frozen; then the vacuum degree is 0.15MPa, and the freez...

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PUM

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Abstract

The invention discloses a preparation method of oryzenin antioxidant peptide, comprising the following steps: (1) screening rice slag with a mesh of 50-200 meshes, and adding water according to the material and water weight ratio of 1:6-12 to be dipped for 10-15 minutes; (2) pouring rice slag feed liquid into a colloid mill to be ground for more than or equal to 2 minutes, and grinding rice slag particles in the feed liquid; (3) adjusting the pH of milled and homogenized feed liquid to 8.0-9.5, adding phosphate buffer, adding alkali protease 200U/g-600U/g rice slag, stirring and carrying out enzymolysis for 1-3 hours at the temperature of 40-65 DEG C, and controlling hydrolyzing degree to be 10-15wt%; (4) killing enzyme and carrying out centrifugal separation; (5) vacuum concentrating, freezing and drying. When adopted to prepare oryzenin antioxidant peptide, the invention has wide raw material resource and low manufacturing cost, the obtained oryzenin antioxidant peptide has high antioxidant activity and high edible safety.

Description

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Claims

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Application Information

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Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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