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Preparation method of composite soft-tissue patch

A soft tissue and patch technology, which is applied in the field of tissue engineering of biomedical materials, can solve problems such as the inability to achieve sustained drug action, complex patch preparation process, and easy immune rejection, so as to promote cell proliferation and matrix secretion, Wide application prospects, excellent tissue reconstruction effect

Active Publication Date: 2013-06-05
SHAANXI RUISHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: the application range of the prepared dermal substitute is limited to skin tissue; the dermal substitute contains cells, which is prone to immune rejection in clinical application, so it is difficult to apply to the repair of in vivo tissues; in addition, the cell-containing The patch preparation process is complicated, the production cost is high, the quality is difficult to control, and the bottleneck of industrialization has not yet been broken through
[0005] Chinese patent (200610153403.5) discloses a surgical patch with bioactive agents that release anti-inflammatory agents, antiplatelet agents, anticoagulants, fibrinolytic agents, cell cycle inhibitors and / or antiproliferative agents function; but it is limited to vascular patches, and is only prepared by a simple drug coating method, which cannot achieve the sustained effect of the drug
[0006] After searching, so far there is no patch that can fully meet the requirements of clinical application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Step 1. Preparation of acellular dermal matrix: Take healthy and fresh pig skin, use a skin drum to remove subcutaneous fat tissue and epidermis, keep a 0.1mm thick dermis, wash it with deionized water, and then wash it with sterile PBS solution. Soak in deionized water at 4°C to fully swell, then freeze at -80°C for more than 30 minutes, take it out and thaw at 37°C until the tissue is completely frozen, repeat twice; use 0.1% (W / V) pancreas Digest the skin with protease solution for 1 day; then wash with 0.02% (W / V) Triton 100 / 200 solution for 1 day; digest with 40 U / ml DNase solution at 37°C for 2 hours; soak the dermis with 1M NaOH solution slices for 1 h; washed with PBS solution and pure water respectively, freeze-dried and sterilized, and stored at 4°C for later use; the porosity of the acellular dermal matrix prepared by this method is 91%;

[0019] Step 2, preparation of cell culture secretion: carry out fibroblast culture with reference to "Principles and Tech...

Embodiment 2

[0024] Step 1. Preparation of acellular dermal matrix: Take healthy fetal cowhide, wash it, manually remove subcutaneous fat tissue and epidermis, and keep a 1mm thick dermis layer, wash it with deionized water, and then wash it with sterile PBS solution, at 4 °C After soaking in deionized water to fully swell, freeze at -80°C for more than 30 minutes, take it out and thaw at 37°C until the tissue is completely frozen, repeat 3 times; digest with 0.25% (W / V) trypsin solution 2 hours, and then washed with 0.05% sodium lauryl sulfate solution for 8 hours; 80U / ml DNase solution was used to digest at 37°C for 2 hours; 1M NaOH solution was used to ablate the dermal slices for 1h; the treated dermis were washed with After being washed with PBS solution and pure water, after being freeze-dried and sterilized, it is stored at 4°C for later use; the porosity of the acellular dermal matrix prepared by this method is 93%;

[0025] Step 2, preparation of cell culture secretion: with refer...

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PUM

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Abstract

The invention discloses a preparation method of a composite soft-tissue patch. Sustained-release microspheres containing human cell culture secretion are mixed with gel solution, then the mixture is compounded with an acellular dermal matrix under vacuum, and after drying and sterilization, the composite soft-tissue patch is obtained. The prepared composite patch can improve the biocompatibility and the bioactivity of the dermal matrix effectively. The experiment shows that compared with the single acellular dermal matrix, the patch can promote cell proliferation and collagen formation effectively and has better biocompatibility and better tissue remolding. The patch can be widely applied to closure of various wound surfaces, repair of various soft-tissue defects and reinforcement of weakplaces and can be prepared into micro particles to be used for filling various fine and weak or dented places by injection, thus having wider application prospect in clinic.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biomedical materials, and in particular relates to a preparation method of a composite tissue patch for soft tissue defect repair. Background technique [0002] The defect and weakness of soft tissue are common problems in clinical practice. The causes include birth defects, pathological factors and aging. The clinical manifestations are: hernia, fistula, scar and wrinkles. This kind of soft tissue defect and weakness usually cannot be repaired by itself, which greatly affects the function and appearance of the corresponding organ tissue. At present, clinical practice mainly relies on tissue patch filling and repairing, so the research and development of tissue patch has become one of the hot spots of modern biomedical materials. [0003] There are many types of tissue patches, classified by material: nylon patch, polypropylene patch, expanded polytetrafluoroethylene patch, biologic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36
Inventor 王爱军
Owner SHAANXI RUISHENG BIOTECH
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